Development of an ultrafast fluorescent probe for specific recognition of hypochlorous acid and its application in live cells

Abstract

Hypochlorous acid (HOCl), a highly potent oxidant of reactive oxygen species, plays critical roles in many physiological and pathological processes. In this work, we designed a simple coumarin-based fluorescent probe, Cou–HOCl, for detecting HOCl in inflammatory cells.

An ICT-FRET-based fluorescent probe for the ratiometric sensing of hypochlorous acid based on a coumarin–naphthalimide derivative

“…[20][21][22] Up to now, numerous fluorescent probes have been developed and applied to the detection of hypochlorous acid. [23][24][25][26][27][28][29][30] Most of these fluorescent probes respond only at a single wavelength, and the fluorescence intensity changes with the concentration of the target analyte, which is easily affected by many factors, such as instrument, environment, probe concentration, photobleaching and excitation intensity. [31][32][33] The ratiometric probes can overcome these shortcomings.…”