Development of Analytical Methods of Spinosad in Agricultural Commodities by HPLC with UV Detector and Monitoring

Kim, Hee-Yun; Choi, Sunhee; Chung, So-Young; Choi, Heeju; Kim, Yong‐Hoon; Cho, Min-Ja; Seo, Eun-Chae; Han, Kyoung-Jin; Choi, Jae‐Suk; Park, Hee-Ok; Ha, Sang-Chul; Shin, Il-Shik; Eom, Ji-Yoon

doi:10.9721/kjfst.2011.43.2.125

Cited by 4 publications

(2 citation statements)

References 9 publications

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“…따라 서 농약을 사용할 때는 그에 따른 사용 기준을 준수하고, 수확한 작물에 잔류하는 양을 미리 설정된 잔류허용기준과 비교하여 해 당 농산물의 유통을 고려하는 것이 필요하다. 최근 식품안전과 관련된 사건사고의 증가는 이러한 안전성을 확보하기 위한 노력 을 더욱 촉진하고 있으며 (3)(4)(5), 세계 각국은 농산물 및 식품에 대 한 잔류 농약의 안전성을 평가하기 위하여 잔류허용기준(maximun residue limit, MRL)을 설정하여 규제할 뿐만 아니라 자국 및 수 입 농산물 중 잔류농약을 분석하고 그 실태를 조사하고 있다 (6). Ametoctradin은 국내에서는 2012년에 신규 등록되어, 식품의약품 안전청에서 포도, 고추 작물에 대해 각각 5.0, 2.0 mg/kg로 국내 잔류허용기준을 신설고시하였으며(제2012-1호), 국제식품규격위원 회(Codex Alimentarius Commission, Codex) 기준은 아직 설정되 지 않았다.…”

Section: 서 론unclassified

Development and Validation of an Analytical Method for Ametoctradin Residue Determination in Domestic Agricultural Commodities by HPLC-PDA

Do¹,

Kwon²,

Lee³

et al. 2013

Korean Journal of Food Science and Technology

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This study was carried out to validate the safety of ametoctradin residues in agricultural commodities by developing an official analysis method. An analytical method was developed and validated using HPLC-PDA detectors. The samples were extracted with methanol, subsequently partitioned with dichloromethane and purified with florisil column chromatograph using acetone/hexane (30/70, v/v) as solvent. The method was validated by using grape, hulled rice, mandarin, and potato spiked with ametoctradin at 0.05 and 5.0 mg/kg, and pepper at 0.05 and 2.0 mg/kg. Average recoveries were 76-114.8% with relative standard deviation less than 10%, and the limit of detection and limit of quantification were 0.0125 and 0.05 mg/kg, respectively. The result of recoveries and overall coefficient of variation of the laboratory results from Gwangju regional Food and Drug Administration (FDA) and Daejeon regional FDA was accorded with Codex Alimentarius Commission Guideline (CAC/GL 40). Based on these results, this method was found to be appropriate for ametoctradin residue determination and can be used as the official method of analysis.

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Section: 서 론unclassified

Development and Validation of an Analytical Method for Ametoctradin Residue Determination in Domestic Agricultural Commodities by HPLC-PDA

Do¹,

Kwon²,

Lee³

et al. 2013

Korean Journal of Food Science and Technology

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“…Spinosad와 spinetoram은 토양 방선균 Saccharopolyspora spinosa의 배양액으로부터 추출 된 천연 살충성분 spinosyn을 화학적으로 변형시켜 만들어진 살충제로 Lepidoptera larvae, Leafminers, Thrips 등의 해충 방제에 널리 사용 (Kim et al, 2011;Malhat, 2013)되지만 대부분 익충에게는 영향력이 적은 것으로 알려져 있다 (Duffield et al, 2000;Sparks et al, 2007; Residue analysis of spinetoram and spinosad on paprika leaf using the modified QuEChERS pre-treatment methods (Park et al, 2012;Liu et al, 2011 (Kwon et al, 2011;Lee et al, 2011;Kim et al, 2012;Park et al, 2014). 최근에는 ECD, NPD, UVD에도 적용 가능한 QuEChERS법 개발도 이루어지고 있으나 (Lee et al, 2012;Yang et al, 2013;Park et al, 2015)…”

Section: Introductionunclassified

Residue analysis of spinetoram and spinosad on paprika leaf using the modified QuEChERS pre-treatment methods

2017

Korean Journal of Agricultural Science

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Spinosad and spinetoram are widely used insecticides for the control of lepidopteran larvae, leaf miners, and thrips; however, they might also have low toxicity toward beneficial insects like bees. Because these pesticides are easily photolyzed by ultraviolet radiation, the QuEChERS method, with its simple pretreatment procedure, is often used for analyzing residues of spinosad and spinetoram. The present study performed a residue analysis using a modified QuEChERS method by pretreating with ammonium salt. The limit of detection (LOD) of the modified method was 0.05 mg/kg and the limit of quantification (LOQ) was 0.25 mg/kg. The coefficient of determination (R 2 ) for the calibration curve was 0.999. Also, we examined any change in the adhesion of spinosad and spinetoram on the plants depending on a spray volume. The adhesion was approximately 70% when the spray volume was increased from 60 L to 120 L per 10 a whereas the adhesion was approximately 37% when the spray volume was increased from 125 L to 250 L. This showed that the amount of adhesion decreased with the higher spray volume. The efficacy result of spinetoram was that over 90% of Frankliniella occidentalis was controlled with the application volume of 125 L per 10 a. Therefore, the result of this study indicates that control of insects is effective and sufficient with a spray volume of 125 L per 10 a in paprika cultivation facilities.

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Storage Properties and Biogenic Amines Production of Makgeolli Brewed with Different Proportions of Rice and Wheat Flour

Kim¹,

Han²

2012

Korean Journal of Food Science and Technology

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Four kinds of makgeolli brewed with different proportions of rice and wheat flour were prepared to investigate the effects of the carbohydrates source on the storage properties, and to examine the chemical characteristics and BA (biogenic amine) contents in makgeolli stored over 30 days at 4, 10 and 20 o C, respectively. The chemical properties and BA contents were sustained at a consistent level for those makgeolli stored at 4 o C over 30 days. The alcohol content was significantly higher for makgeolli brewed with higher proportions of rice (R) over wheat flour (WF) (R 100: WF 0, R 70: WF 30) when compared with those brewed with a high proportion of wheat flour (R 30: WF 70, R 0: WF 100), and increased with increasing storage temperature and duration. The free amino acid contents of makgeolli brewed with high proportion of rice were significantly lower under the same conditions. Among the 8 BAs examined, tyramine, putrescine, cadaverine and phenylethylamine were detected, while tyramine and putrescine were mainly detected. Histamine was not detected throughout storage. Makgeolli brewed with rice as the only carbohydrate source exhibited very low levels of total BA during 30 days of storing at 20 o C. Total BA contents of R100, R70, R30, R0 were 178.6, 682.9, 1186.7 and 1150.4 mg/L, respectively. These results suggest that makgeolli brewed with relatively higher proportions of rice have better storage properties and are safer for consumption.

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Development of Analytical Methods of Spinosad in Agricultural Commodities by HPLC with UV Detector and Monitoring

Cited by 4 publications

References 9 publications

Development and Validation of an Analytical Method for Ametoctradin Residue Determination in Domestic Agricultural Commodities by HPLC-PDA

Development and Validation of an Analytical Method for Ametoctradin Residue Determination in Domestic Agricultural Commodities by HPLC-PDA

Residue analysis of spinetoram and spinosad on paprika leaf using the modified QuEChERS pre-treatment methods

Storage Properties and Biogenic Amines Production of Makgeolli Brewed with Different Proportions of Rice and Wheat Flour

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