Development of antibiotic resistance genes in microbial communities during long-term operation of anaerobic reactors in the treatment of pharmaceutical wastewater

Aydın, Sevcan; İnce, Bahar; İnce, Orhan

doi:10.1016/j.watres.2015.07.007

Cited by 162 publications

(57 citation statements)

References 37 publications

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“…Previous study has demonstrated that the outputs from the qPCR and metagenomic analyses support the same results for detecting ARGs no matter in their total quantity or individual ARG abundance (Aydin et al, 2015). In this study, both metagenomic and qPCR analyses showed that thermophilic sludge held a lower overall ARG abundance than that of the mesophilic one (Figs.…”

Section: Discussionsupporting

confidence: 75%

“…The specificities of the used primer sets (Table S1) have been validated in recently published papers (Ma et al, 2011;Liu et al, 2012Liu et al, , 2014Aydin et al, 2015) and verified in this study. Standard plasmids carrying target genes were obtained by TA clones and extracted using a TIANpure Mini Plasmid kit (Tiangen, China).…”

Section: Quantitative Pcr Assaysupporting

confidence: 56%

“…Integrons, usually located in transposons or plasmids (Guerin et al, 2009), are important genetic elements for ARG recombination, and can acquire and express exogenous ARGs embedded within gene cassettes to accelerate their transfer (Stokes and Hall, 1989). As the most abundant integron in environmental bacteria (Stalder et al, 2014), intI1 has been suggested as a proxy for antibiotic pollution (Gillings et al, 2015;Aydin et al, 2015). In this study, all integron related sequences in sludge metagenomes belonged to intI1 and their abundances moderately decreased after temperature increase.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, metagenomic analysis was applied and revealed that thermophilic sludge had a slightly lower resistome than that of mesophilic sludge . Recent studies also showed that the combination of metagenomics and qPCR might provide a more comprehensive and quantitative insight into the dynamics of microbial population and ARGs during wastewater and sludge treatment (Aydin et al, 2015;Zhang et al, 2015;Ju et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Changes of resistome, mobilome and potential hosts of antibiotic resistance genes during the transformation of anaerobic digestion from mesophilic to thermophilic

et al. 2016

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a b s t r a c tThis study aimed to reveal how antibiotic resistance genes (ARGs) and their horizontal and vertical transfer-related items (mobilome and bacterial hosts) respond to the transformation of anaerobic digestion (AD) from mesophilic to thermophilic using one-step temperature increase. The resistomes and mobilomes of mesophilic and thermophilic sludge were investigated using metagenome sequencing, and the changes in 24 representative ARGs belonging to three categories, class 1 integron and bacterial genera during the transition period were further followed using quantitative PCR and 454-pyrosequencing. After the temperature increase, resistome abundance in the digested sludge decreased from 125.97 ppm (day 0, mesophilic) to 50.65 ppm (day 57, thermophilic) with the reduction of most ARG types except for the aminoglycoside resistance genes. Thermophilic sludge also had a smaller mobilome, including plasmids, insertion sequences and integrons, than that of mesophilic sludge, suggesting the lower horizontal transfer potential of ARGs under thermophilic conditions. On the other hand, the total abundance of 18 bacterial genera, which were suggested as the possible hosts for 13 ARGs through network analysis, decreased from 23.27% in mesophilic sludge to 11.92% in thermophilic sludge, indicating fewer hosts for the vertical expansion of ARGs after the increase in temperature. These results indicate that the better reduction of resistome abundance by thermophilic AD might be associated with the decrease of both the horizontal and vertical transferability of ARGs.

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Section: Discussionsupporting

confidence: 75%

Section: Quantitative Pcr Assaysupporting

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Changes of resistome, mobilome and potential hosts of antibiotic resistance genes during the transformation of anaerobic digestion from mesophilic to thermophilic

et al. 2016

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“…Anaerobic biodegradation uses this potential to produce methane through several biochemical processes where many different species of anaerobic bacteria and archaea involved (Arikan 2008;Álvarez et al 2010). Therefore, the system itself is delicate and susceptible to inhibitors, which may hinder performance of the process (Lallai et al 2002;Aydin et al 2015a;Lins et al 2015). Antibiotics in manure cause a problem because of the loss of energy recovery not only in digesters but also in soil when they are applied as fertilizers.…”

Section: Introductionmentioning

confidence: 99%

Changes in microbial community structures due to varying operational conditions in the anaerobic digestion of oxytetracycline-medicated cow manure

Türker

Aydın

Akyol

et al. 2016

Appl Microbiol Biotechnol

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Management of manure containing veterinary antibiotics is a major concern in anaerobic treatment systems because of their possible adverse effects on microbial communities. Therefore, the aim of study was to investigate how oxytetracycline (OTC) influences bacteria and acetoclastic and hydrogenotrophic methanogens under varying operational conditions in OTC-medicated and non-medicated anaerobic cow manure digesters. Concentrations of OTC and its metabolites throughout the anaerobic digestion were determined using ultraviolet-high-performance liquid chromatography (UV-HPLC) and tandem liquid chromatography-mass spectrometry (LC/MS/MS), respectively. Fluorescent in situ hybridization, denaturing gradient gel electrophoresis, cloning, and sequencing analyses were used to monitor changes in microbial community structures. According to the results of analytical and molecular approaches, operating conditions highly influence active microbial community dynamics and associate with biogas production and elimination of OTC and its metabolites during anaerobic digestion of cow manure in the presence of an average initial concentration of 2.2 mg OTC/L. The impact of operating conditions has a drastic effect on acetoclastic methanogens than hydrogenotrophic methanogens and bacteria.

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The Mobile Resistome in Wastewater Treatment Facilities and Downstream Environments

Marano

2017

Antimicrobial Resistance in Wastewater Treatment Processes

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Development of antibiotic resistance genes in microbial communities during long-term operation of anaerobic reactors in the treatment of pharmaceutical wastewater

Cited by 162 publications

References 37 publications

Changes of resistome, mobilome and potential hosts of antibiotic resistance genes during the transformation of anaerobic digestion from mesophilic to thermophilic

Changes of resistome, mobilome and potential hosts of antibiotic resistance genes during the transformation of anaerobic digestion from mesophilic to thermophilic

Changes in microbial community structures due to varying operational conditions in the anaerobic digestion of oxytetracycline-medicated cow manure

The Mobile Resistome in Wastewater Treatment Facilities and Downstream Environments

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