Development of antibody ELISA specific of Leptospira interrogans serovar Grippotyphosa, Canicola, and Icterohaemorrhagiae to monitor vaccine immunogenicity

Cariou, C.; Herbet, Gaëtan; Ripart, Prescillia; Martin-Cagnon, Nathalie; Bouvet, J.; Schneider, Michèle; Guiot, Anne-Laure; Cupillard, Lionel

doi:10.1016/j.vetimm.2019.109960

Cited by 8 publications

(8 citation statements)

References 21 publications

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“…The leptospiral serum antibody content of animals reflects exposure to immunodominant Leptospira antigens during infection and vaccination. Leptospiral surface proteins are often cross-reactive, while the surface lipopolysaccharide (LPS) components are mostly serovar-specific and generate antibodies specific for those serovars [ 62 , 63 , 64 , 65 , 66 ]. Previous studies on antibody detection in dogs have used sera from animals that have either been infected or have received serovar-specific vaccines [ 42 , 53 , 57 , 65 , 66 , 67 ].…”

Section: Discussionmentioning

confidence: 99%

“…Leptospiral surface proteins are often cross-reactive, while the surface lipopolysaccharide (LPS) components are mostly serovar-specific and generate antibodies specific for those serovars [ 62 , 63 , 64 , 65 , 66 ]. Previous studies on antibody detection in dogs have used sera from animals that have either been infected or have received serovar-specific vaccines [ 42 , 53 , 57 , 65 , 66 , 67 ]. In the current study, the canine sera used were chosen from various types of dogs from areas where there were different circulating serovars.…”

Section: Discussionmentioning

confidence: 99%

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Improved Antibody Detection for Canine Leptospirosis: ELISAs Modified Using Local Leptospiral Serovar Isolates from Asymptomatic Dogs

Boonciew,

Saisongkorh,

Brameld

et al. 2024

Animals

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Leptospirosis is a zoonotic disease of significant concern for human and animal health, with domestic animals, including dogs, acting as reservoirs for human infection. Serology is widely used for leptospirosis diagnosis, even though the standard microscopic agglutination test (MAT) using a panel of serovars lacks specificity and can lead to detection limitations in certain regions. In this study, we aimed to develop an antibody detection tool for dogs using an indirect enzyme-linked immunosorbent assay (ELISA) with a set of local serovar isolates, including Paidjan, Dadas, and Mini, to enhance the accuracy of leptospirosis surveillance in our region. The specificity and sensitivity of various antigen preparations, namely leptospiral whole-cell protein (WCP), total membrane protein (TMP), and outer membrane protein (OMP), were assessed using sera from infected and non-infected dogs, as well as negative puppy sera. Leptospirosis diagnosis was supported using a genus-specific nested polymerase chain reaction test on all collected sera. Protein preparations were validated using SDS-PAGE and Western blotting analysis. In the results, the standard MAT failed to detect antibodies in any of the dogs confirmed as being infected using PCR and isolation, highlighting its limitations. In contrast, the OMP-based ELISAs using local isolates of Leptospira serovars gave positive results with sera from all infected dogs, and negative results with sera from all dogs from non-endemic areas. IgG titres of infected and unvaccinated dogs from endemically affected areas were significantly higher than those in non-endemic regions. Using the OMP-based IgG/ELISAs with the local serovar Dadas resulted in higher specificity and lower sensitivity than when using the WCP- and TMP-based IgG/ELISAs. Agreement analysis revealed fair and moderate concordance between OMP-based IgG/ELISAs and PCR results, whereas slight and fair agreement was observed between OMP-based ELISAs and the MAT. Overall, the modified OMP-based IgG/ELISAs, utilising relevant local serovar isolates from dogs, demonstrated improved accuracy in detecting leptospirosis in the study area, overcoming the limitations of the MAT. This study highlights the importance of identifying and incorporating these local circulating serovar isolates into serological techniques for leptospirosis diagnosis and surveillance.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Improved Antibody Detection for Canine Leptospirosis: ELISAs Modified Using Local Leptospiral Serovar Isolates from Asymptomatic Dogs

Boonciew,

Saisongkorh,

Brameld

et al. 2024

Animals

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“…Despite, MAT still being suited for diagnosing recent leptospirosis, findings suggests that careful assessment of antibody titers in association with the vaccination history of a patient that is suspected of having clinical leptospirosis is needed as higher titers during the early stages of vaccinations may mask true infection (Klaasen and Adler 2015). The sensitivity of MAT is very much dependent on the test method and the subjective readout by a technician (Cariou et al 2020) which is improved by the built in positive and negative controls to mitigate error (Barr et al 2005).…”

Section: Discussionmentioning

confidence: 99%

Post-Vaccination Leptospiral Antibody Titers among Pet Dogs in Malaysia

2022

Int J Vet Sci

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Vaccine immunity among Malaysian dogs with its diverse circulating serovars, is still debatable. The recommended vaccination protocol was implemented on a local level, but there was little local documentation of vaccine antibody titer production. This study evaluated the duration and magnitude of antibody titers before, during and after vaccination in both overtly healthy puppies and adult dogs. Puppies (n=20; 6-8 weeks) and adult dogs (n=20; 1-5 years old) were recruited. The dogs were vaccinated with a commercial quadrivalent vaccine (Icterohaemorrhagiae, Canicola, Grippotyphosa and Pomona) according to guidelines [World Small Animal Veterinary Association (WSAVA)]. Blood was sampled at pre-and post-vaccination up to one-year duration at intervals of 2, 4, 6, 8, 10, 12 weeks followed by 6, 9, 12 months. Serum was tested against the vaccine serovars using microscopic agglutination test (MAT) while whole blood for polymerase chain reaction (PCR). The antibody titer in puppies ranged between 1:50 to 1:200 was present for 6 months whereas the adults had higher titer of between 1:50 to 1:800 for a duration of one year. The antibody titers detected was the highest and persisted the longest for serovar Icterohaemorrhagiae followed by serovars Canicola, Grippotyphosa and Pomona. All dogs were PCR negative throughout the study period. Consideration should be taken when interpreting MAT results of clinical cases with presence of vaccine antibodies, requiring paired serum for better serodiagnosis. MAT remains a gold standard serological method for diagnosis and surveillance, current findings may become a basis of comparison allowing better interpretation and improved usability.

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“…Other analytical techniques such as serogroup specific ELISA ( SumanthKumar et al, 2013 ) are interesting to better characterize antibody response against Leptospira. Internally developed ELISA tests were therefore used for serogroups Canicola, Icterohaemorrhagiae, Grippotyphosa ( Cariou et al, 2020 ). Due to their higher sensitivity, these tests enable a better follow-up of post-vaccinal antibodies.…”

Section: Discussionmentioning

confidence: 99%

“…Anti-leptospiral antibodies for serovars Canicola, Icterohaemorrhagiae and Grippotyphosa, were also titrated by a proprietary blocking ELISA as described in a recent publication ( Cariou et al, 2020 ). Antibodies against Leptospira Pomona were titrated by microagglutination technique (MAT) at the Institut Pasteur, National Reference Center for Leptospirosis, Paris (France)…”

Section: Methodsmentioning

confidence: 99%

Compatibility between a rabies vaccine and two canine combined vaccines against canine distemper, adenovirosis, parvovirosis, parainfluenza virus disease and leptospirosis, with or without canine coronavirus

Thibault¹,

Bouvet

Cupillard

et al. 2022

Comparative Immunology, Microbiology and Infectious Diseases

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Development of antibody ELISA specific of Leptospira interrogans serovar Grippotyphosa, Canicola, and Icterohaemorrhagiae to monitor vaccine immunogenicity

Cited by 8 publications

References 21 publications

Improved Antibody Detection for Canine Leptospirosis: ELISAs Modified Using Local Leptospiral Serovar Isolates from Asymptomatic Dogs

Improved Antibody Detection for Canine Leptospirosis: ELISAs Modified Using Local Leptospiral Serovar Isolates from Asymptomatic Dogs

Post-Vaccination Leptospiral Antibody Titers among Pet Dogs in Malaysia

Compatibility between a rabies vaccine and two canine combined vaccines against canine distemper, adenovirosis, parvovirosis, parainfluenza virus disease and leptospirosis, with or without canine coronavirus

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