Development of Antifouling and Bactericidal Coatings for Platelet Storage Bags Using Dopamine Chemistry

Abstract

Platelets have a limited shelf life, due to the risk of bacterial contamination and platelet quality loss. Most platelet storage bags are made of a mixture of polyvinyl chloride with a plasticizer, denoted as pPVC. To improve biocompatibility of pPVC with platelets and to inhibit bacterial biofilm formation, an antifouling polymer coating is developed using mussel-inspired chemistry. A copolymer of N,N-dimethylacrylamide and N-(3-aminopropyl)methacrylamide hydrochloride is synthesized and coupled with catechol groups, named DA51-cat. Under mild aqueous conditions, pPVC is first equilibrated with an anchoring polydopamine layer, followed by a DA51-cat layer. Measurements show this coating decreases fibrinogen adsorption to 5% of the control surfaces. One-step coating with DA51-cat does not coat pPVC efficiently although it is sufficient for coating silicon wafers and gold substrates. The dual layer coating on platelet bags resists bacterial biofilm formation and considerably decreases platelet adhesion. A cationic antimicrobial peptide, E6, is conjugated to DA51-cat then coated on silicon wafers and introduces bactericidal activity to these surfaces. Time-of-flight second ion-mass spectroscopy is successfully applied to characterize these surfaces. pPVC is widely used in medical devices; this method provides an approach to controlling biofouling and bacterial growth on it without elaborate surface modification procedures.

Multifunctional and Transformable ‘Clickable’ Hydrogel Coatings on Titanium Surfaces: From Protein Immobilization to Cellular Attachment

“…Reactive polymeric coatings are at the heart of various biomedical applications, which range from sensing and delivery platforms to facilitate the adaptation of various implant materials. Widely used applications in biomedical sciences encompass coating of orthopedic materials, cardiovascular stents, antibacterial surfaces, drug delivery devices, tissue engineering scaffolds and biosensors [ 15 , 16 ]. Using hydrogels as a coating material is quite attractive since they provide a 3-dimensional (3D) soft material-based interface, whose properties can be tuned by the choice of the coating material and can be further augmented by appropriate functionalization [ 17 , 18 , 19 , 20 , 21 , 22 , 23 ].…”

Bioactive and Bioadhesive Catechol Conjugated Polymers for Tissue Regeneration

“…Subsequently, catechol molecule was conjugated to those copolymers by reaction between the chloride acid of HCA (previously prepared) and a portion of the hydroxyl groups of the H units in the copolymers (Figure 2). Several catechol-containing synthetic polymers have been recently developed in the family of polymethacrylates and polymethacrylamides [41,60,61,62,63]. Some of them are obtained from the synthesis of monomers containing the catechol moiety, their purification and finally their subsequent co/polymerization.…”

“…These terpolymers possess a hydrophilic character amiable with the environment of a skin lesion. Although different polymeric materials with catechol functionality have previously been reported [41,60,61,62,63], the novelty of the obtained terpolymers lies in the pathway via postpolymerization conjugation reaction to provide a flexible long-arm catechol conjugated polymer with enhanced availability of the catechol side groups. This pathway has the advantages of avoiding the drawbacks of the scavenger activity of catechol groups in polymerization reactions, protection of catechol groups is not required, while providing high yield.…”

Bioactive and Bioadhesive Catechol Conjugated Polymers for Tissue Regeneration

“…These terpolymers possess a hydrophilic character amiable with the environment of a skin lesion. Although different polymeric materials with catechol functionality have previously been reported [41,[60][61][62][63], the novelty of the obtained terpolymers lies in the pathway via postpolymerization conjugation reaction to provide a flexible long-arm catechol conjugated polymer with enhanced availability of the catechol side groups. This pathway has the advantages of avoiding the drawbacks coming to the scavenger bubbled for 1 min.…”

“…Subsequently, catechol molecule has been conjugated to those copolymers by reaction between the chloride acid of HCA (previously prepared) and a portion of the hydroxyl groups of the HEMA units in the copolymers (Figure 2). Several catechol containing synthetic polymers have been recently developed in the family of polymethacrylates and polymethacrylamides[41,[60][61][62][63]. Some of them are obtained from the synthesis of monomers containing the catechol moiety, their purification and finally their subsequent co/polymerization.…”