Development of antimicrobial resistance in the normal anaerobic microbiota during one year after administration of clindamycin or ciprofloxacin

Rashid, Mamun Ur; Weintraub, Andrej; Nord, Carl Erik

doi:10.1016/j.anaerobe.2014.10.004

Cited by 28 publications

(22 citation statements)

References 24 publications

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“…Despite the marked increase in the recovery of clindamycin-resistant anaerobic strains in Costa Rica in the last decade, 35 , 36 this antibiotic is still the first-choice antibiotic for infections by anaerobic bacteria in Costa Rica and other geographic areas. 37 The alarming clindamycin resistance level of C. difficile observed in this study (88%) is slightly lower than the level recorded during a 2009 outbreak at another major hospital (97%) 18 but is still much higher than the values reported from other latitudes. 38 , 39 Almost half of the strains, all of which belonged to genotypes NAP CR1 and NAP9, had MICs >256 μg/mL, whereas the remaining strains had MICs=8 μg/mL.…”

Section: Discussioncontrasting

confidence: 62%

Predominance and high antibiotic resistance of the emerging Clostridium difficile genotypes NAP _CR1 and NAP9 in a Costa Rican hospital over a 2-year period without outbreaks

López-Ureña

Quesada-Gómez

Montoya-Ramírez

et al. 2016

Emerging Microbes & Infections

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Clostridium difficile is the major causative agent of nosocomial antibiotic-associated diarrhea. In a 2009 outbreak of C. difficile-associated diarrhea that was recorded in a major Costa Rican hospital, the hypervirulent NAP1 strain (45%) predominated together with a local genotype variant (NAPCR1, 31%). Both strains were fluoroquinolone-resistant and the NAPCR1 genotype, in addition, was resistant to clindamycin and rifampicin. We now report on the genotypes and antibiotic susceptibilities of 68 C. difficile isolates from a major Costa Rican hospital over a 2-year period without outbreaks. In contrast to our previous findings, no NAP1 strains were detected, and for the first time in a Costa Rican hospital, a significant fraction of the isolates were NAP9 strains (n=14, 21%). The local NAPCR1 genotype remained prevalent (n=18, 26%) and coexisted with 14 strains (21%) of classic hospital NAP types (NAP2, NAP4, and NAP6), eight new genotypes (12%), four environmental strains classified as NAP10 or NAP11 (6%), three strains without NAP designation (4%) and seven non-toxigenic strains (10%). All 68 strains were resistant to ciprofloxacin, 88% were resistant to clindamycin and 50% were resistant to moxifloxacin and rifampicin. Metronidazole and vancomycin susceptibilities were universal. The NAPCR1 and NAP9 strains, which have been associated with more severe clinical infections, were more resistant to antibiotics than the other strains. Altogether, our results confirm that the epidemiology of C. difficile infection is dynamic and that A−B+ strains from the NAP9 type are on the rise not only in the developed world. Moreover, our results reveal that the local NAPCR1 strains still circulate in the country without causing outbreaks but with equally high antibiotic-resistance rates and levels.

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Section: Discussioncontrasting

confidence: 62%

Predominance and high antibiotic resistance of the emerging Clostridium difficile genotypes NAP _CR1 and NAP9 in a Costa Rican hospital over a 2-year period without outbreaks

López-Ureña

Quesada-Gómez

Montoya-Ramírez

et al. 2016

Emerging Microbes & Infections

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“…might exist in pilosebaceous follicles before the apparition of acne and might explain the failure of first-line antibiotic treatments. Considering the increasing levels of resistant bacteria and the effect of long-term antibiotics treatments on the microbiota, 39 this phenomenon should be investigated in more details. Furthermore, we confirmed that the phylotype IA was clearly associated to erythromycin resistance, as a high proportion of C. acnes EryR isolates, especially from acne individuals, were phylotype IA strains (Fig.…”

Section: Discussionmentioning

confidence: 99%

Characterisation of Cutibacterium acnes phylotypes in acne and in vivo exploratory evaluation of Myrtacine^®

Pécastaings

Roques

Nocera

et al. 2018

Acad Dermatol Venereol

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Objective Our main objective was to compare Cutibacterium acnes (C. acnes) skin colonisation in patients with mild to moderate acne versus healthy controls and secondly, to evaluate a Myrtacine â -based cream on C. acnes total population and antibioresistant Cutibacteria in patients with acne.Methods In 60 acne patients (Global Acne Severity Scale, GEA grades 2-3), of mean age 20 [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30] Results We first showed (i) high and similar levels of C. acnes colonisation in superficial pilosebaceous follicles and detection of EryR and ClnR strains in both acne and control groups; (ii) different repartition of phylotypes in acne patients versus healthy control, with a predominance of phylotype IA in acne patients and a link between phylotype IA and erythromycin resistance. Besides, after treatment with the Myrtacine â -based cream in acne patients, there was no change in C. acnes total load, but a significant decrease of EryR Cutibacteria, reduced porphyrin production by C. acnes, a decrease in acne severity (GEA), associated with reduced retentional and inflammatory lesions.Conclusion Cutibacterium acnes colonisation was not significantly different in acne versus control groups. Phylotype IA was predominant in acne patient and in EryR C. acnes. A Myrtacine â -based cream significantly reduced the level of EryR Cutibacteria in vivo and improved acne lesions.

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“…It should be noticed that the antimicrobials caused different collateral effects on microbial community. For example, the proportion of the genus Bacteroides significantly increased after ciprofloxacin administration in human study [45]. The human study found that the children have less stable communities after receiving 9 to 15 antimicrobial treatments in the first 3 years of life compared with the children never received antimicrobials [46].…”

Section: Discussionmentioning

confidence: 93%

Effects of pulsed in-feed antimicrobials on chicken fecal resistome

Chen¹,

Yang²,

Zeng³

et al. 2019

Preprint

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Background: Antimicrobial resistance has become a global problem that poses great threats to human health. Antimicrobials are widely used in broiler chicken production and consequently affect the fecal resistome. Here we used metagenomic approach to investigate the effects of pulsed antimicrobial administration on chicken fecal resistome. Chickens received three 5-day-course of alone/combined antimicrobials at therapeutic dose, including amoxicillin, chlortetracycline and florfenicol. Chicken feces was collected on T0 (before treatment), T5 (after first treatment), T10 (after second treatment), T15 (after third treatment) at the chicken age of 40 days that are ready for slaughter. Results: Tetracycline resistance gene and polymyxin resistance gene only responded to chlortetracycline and amoxicillin, respectively. The tetracycline resistance gene tetC significantly increased from T0 to T15 in chlortetracycline group. The multidrug resistance gene acrB, acrF, mdtA, mdtK and CRP only occurred significant increase in amoxicillin group (P<0.05). Klebsiella significantly decreased from T0 to T10 in florfenicol group, while significantly increased from T0 to T15 in chlortetracycline group (P<0.05). Amoxicillin did not lead significant change to Klebsiella. Escherichia was always the major host for multidrug resistance genes. ARG-harboring Escherichia increased mainly due to the antimicrobial selection for β-lactam, tetracycline, chloramphenicol resistance genes they harbored. Conclusions: The results indicated that the effects induced by amoxicillin, chlortetracycline and florfenicol significantly shaped the fecal microbiome and resistome. We provided a comprehensive insight into antimicrobial-mediated alteration of chicken fecal microbiome and resistome. These findings will give suggests to veterinarian for combating antimicrobial resistance in broiler chicken production.

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Development of antimicrobial resistance in the normal anaerobic microbiota during one year after administration of clindamycin or ciprofloxacin

Cited by 28 publications

References 24 publications

Predominance and high antibiotic resistance of the emerging Clostridium difficile genotypes NAP _CR1 and NAP9 in a Costa Rican hospital over a 2-year period without outbreaks

Predominance and high antibiotic resistance of the emerging Clostridium difficile genotypes NAP _CR1 and NAP9 in a Costa Rican hospital over a 2-year period without outbreaks

Characterisation of Cutibacterium acnes phylotypes in acne and in vivo exploratory evaluation of Myrtacine^®

Effects of pulsed in-feed antimicrobials on chicken fecal resistome

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Development of antimicrobial resistance in the normal anaerobic microbiota during one year after administration of clindamycin or ciprofloxacin

Cited by 28 publications

References 24 publications

Predominance and high antibiotic resistance of the emerging Clostridium difficile genotypes NAP CR1 and NAP9 in a Costa Rican hospital over a 2-year period without outbreaks

Predominance and high antibiotic resistance of the emerging Clostridium difficile genotypes NAP CR1 and NAP9 in a Costa Rican hospital over a 2-year period without outbreaks

Characterisation of Cutibacterium acnes phylotypes in acne and in vivo exploratory evaluation of Myrtacine®

Effects of pulsed in-feed antimicrobials on chicken fecal resistome

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Predominance and high antibiotic resistance of the emerging Clostridium difficile genotypes NAP _CR1 and NAP9 in a Costa Rican hospital over a 2-year period without outbreaks

Predominance and high antibiotic resistance of the emerging Clostridium difficile genotypes NAP _CR1 and NAP9 in a Costa Rican hospital over a 2-year period without outbreaks

Characterisation of Cutibacterium acnes phylotypes in acne and in vivo exploratory evaluation of Myrtacine^®