Development of antral follicles in cryopreserved cat ovarian tissue transplanted to immunodeficient mice

Bosch, Pablo; Hernández–Fonseca, Hugo; Miller, Doris M.; Wininger, J. David; Massey, J.H.; Lamb, Steven V; Brackett, Benjamin G.

doi:10.1016/s0093-691x(03)00244-9

Cited by 66 publications

(52 citation statements)

References 33 publications

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“…In animals, ovarian tissue cryopreservation and transplantation have been used for breeding, research, and conservation purposes in various species, including mouse, rats, rabbits, sheep, cats, wombats, and monkeys (Candy et al 1995, Aubard et al 1998, Wolvekamp et al 2001, Almodin et al 2004, Bosch et al 2004. Slow-cooling, usually the method of choice for cryopreservation of ovarian tissue, is based on the principle of optimizing cooling rates to control injury from ice crystal formation, while minimizing chemical toxicity and osmotic stress from exposure to high concentrations of salts and solutes during freezing .…”

Section: Introductionmentioning

confidence: 99%

Live offspring from vitrified blastocysts derived from fresh and cryopreserved ovarian tissue grafts of adult mice

Wang¹,

Catt²,

Pangestu³

et al. 2009

Reproduction

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Ovarian tissue cryopreservation and transplantation can be used to preserve fertility for cancer patients. In this study, we assessed the viability and function of ovarian tissue from adult mice that was cryopreserved by solid surface vitrification or traditional slow-cooling using various in vitro and in vivo techniques, including allotransplantation, in vitro oocyte maturation, embryo culture in vitro, blastocyst cryopreservation, embryo transfer, and development. The importance of cumulus cells for oocyte maturation, fertilization, and embryo development was investigated. Graft recovery, follicle survival, and oocyte retrieval was similar in control, vitrified, and slow-cooled groups. High rates of oocyte maturation, cleavage, and blastocyst formation were achieved, with no significant differences between the control, vitrified or slow-cooled ovarian tissue grafts. The presence of cumulus cells was important for oocyte maturation, fertilization, and subsequent development. Cumulus-oocyte complexes with no surrounding cumulus cells (N-COCs) or with an incomplete layer (P-COCs) had significantly lower rates of oocyte maturation and blastocyst formation than cumulus-oocyte complexes with at least one complete layer of cumulus cells (F-COCs; maturation rate: 63, 78 vs 94%; blastocyst rate: 29, 49 vs 80%). Live births were achieved using vitrified blastocysts derived from oocytes taken from vitrified and slow-cooled ovarian tissue heterotypic allografts. Successful production of healthy offspring from these vitrified blastocysts suggests that this technique should be considered as a useful stage to pause in the assisted reproduction pathway. This provides an alternative protocol for restoring fertility and offering cancer patients a better indication of their chances of pregnancy and live birth.

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Section: Introductionmentioning

confidence: 99%

Live offspring from vitrified blastocysts derived from fresh and cryopreserved ovarian tissue grafts of adult mice

Wang¹,

Catt²,

Pangestu³

et al. 2009

Reproduction

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“…Nevertheless, several authors use cryopreservation protocols with a direct immersion into liquid nitrogen after -40°C, such as Rodrigues et al (2004) in the goat, Lucci et al (2004) in the zebu cow, in the ewe or Lima et al (2006) in the cat. Births had been obtained after graft of ovarian fragments frozen with such a protocol in the rabbit doe (Almodin et al, 2004b) and in the ewe (Almodin et al, 2004a), but not in the cat where in vivo follicular growth were observed when using a freezing protocol with controlled third phase (Bosch et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

New Approaches of Ovarian Tissue Cryopreservation from Domestic Animal Species

Neto¹,

Joly²,

Commin³

et al. 2012

Current Frontiers in Cryopreservation

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“…However, ovulation was not observed in any of the grafts. Furthermore, Bosch et al (2002) reported that freeze-thawed cat ovarian cortex not only survives xenotransplantation into the kidney capsule from severe combined immunodefficient mice but also contains follicles able to grow to antral stages containing gonadotropin responsive granulosa cells.…”

Section: Ovarian Tissue Preservation and Culturementioning

confidence: 99%