Development of assisted reproductive technologies for<i>Mus spretus</i>†

Hasegawa, Ayumi; Mochida, Keiichi; Matoba, Shogo; Inoue, Kimiko; Hama, Daiki; Kadota, Masayo; Hiraiwa, Noriko; Yoshiki, Atsushi; Ogura, Atsuo

doi:10.1093/biolre/ioaa177

Cited by 6 publications

(5 citation statements)

References 49 publications

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“…It is also important to note that the IVF rate when using M. spretus sperm was found to be significantly lower than seen when using C57BL/6J sperm, potentially because the Infrafrontier protocols used are optimized for the C57BL/6J strain. Potentially, new protocol improvements in assisted reproductive techniques optimized for M. spretus may help improve these rates 12 .…”

Section: Discussionmentioning

confidence: 99%

Naturally sterile Mus spretus hybrids are suitable for the generation of pseudopregnant embryo transfer recipients

Preece,

Biggs,

Grencis

et al. 2024

Lab Anim

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For the preparation of embryo transfer recipients, surgically vasectomized mice are commonly used, generated by procedures associated with pain and discomfort. Sterile transgenic strains provide a nonsurgical replacement, but their maintenance requires breeding and genotyping procedures. We have previously reported the use of naturally sterile STUSB6F1 hybrids for the production of embryo transfer recipients and found the behavior of these recipients to be indistinguishable from those generated by vasectomized males. The method provides two substantial 3R impacts: refinement (when compared with surgical vasectomy) and reduction in breeding procedures (compared with sterile transgenic lines). Despite initial promise, the 3Rs impact of this innovation was limited by difficulties in breeding the parental STUS/Fore strain, which precluded the wider distribution of the sterile hybrid. The value of a 3R initiative is only as good as the uptake in the community. Here we, thus, select a different naturally sterile hybrid, generated from strains that are widely available: the B6SPRTF1 hybrid between C57BL/6J and Mus spretus. We first confirmed its sterility by sperm counting and testes weight and then trialed the recovery of cryopreserved embryos and germplasm within three UK facilities. Distribution of sperm for the generation of these hybrids by in vitro fertilization was found to be the most robust distribution method and avoided the need to maintain a live M. spretus colony. We then tested the suitability of B6SPRTF1 sterile hybrids for the generation of embryo transfer recipients at these same three UK facilities and found the hybrids to be suitable when compared with surgical vasectomized mice and a sterile transgenic strain. In conclusion, the potential 3Rs impact of this method was confirmed by the ease of distribution and the utility of sterile B6SPRTF1 hybrids at independent production facilities.

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Section: Discussionmentioning

confidence: 99%

Naturally sterile Mus spretus hybrids are suitable for the generation of pseudopregnant embryo transfer recipients

Preece,

Biggs,

Grencis

et al. 2024

Lab Anim

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“…We also found that use of pseudopregnant ICR females as recipients did not compromise development of chimeric embryos. We have previously reported that transfer of embryos from the MSM strain and SPR2 ( M. spretus ) into laboratory females needed cyclosporin A, an immunosuppressant, or (B6 × C3H) recipient females to avoid immuno-rejection by mothers 32 , 33 . It was probable that the origin of the placenta from laboratory mice in our chimeric mice could minimize the immunological attack from the mother.…”

Section: Discussionmentioning

confidence: 99%

Derivation of embryonic stem cells from wild-derived mouse strains by nuclear transfer using peripheral blood cells

Watanabe

Hirose

Hasegawa

et al. 2023

Sci Rep

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Wild-derived mouse strains have been extensively used in biomedical research because of the high level of inter-strain polymorphisms and phenotypic variations. However, they often show poor reproductive performance and are difficult to maintain by conventional in vitro fertilization and embryo transfer. In this study, we examined the technical feasibility of derivation of nuclear transfer embryonic stem cells (ntESCs) from wild-derived mouse strains for their safe genetic preservation. We used leukocytes collected from peripheral blood as nuclear donors without sacrificing them. We successfully established 24 ntESC lines from two wild-derived strains of CAST/Ei and CASP/1Nga (11 and 13 lines, respectively), both belonging to Mus musculus castaneus, a subspecies of laboratory mouse. Most (23/24) of these lines had normal karyotype, and all lines examined showed teratoma formation ability (4 lines) and pluripotent marker gene expression (8 lines). Two male lines examined (one from each strain) were proven to be competent to produce chimeric mice following injection into host embryos. By natural mating of these chimeric mice, the CAST/Ei male line was confirmed to have germline transmission ability. Our results demonstrate that inter-subspecific ntESCs derived from peripheral leukocytes could provide an alternative strategy for preserving invaluable genetic resources of wild-derived mouse strains.

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“…Nevertheless, although it is possible that the sperm cryopreservation process might skew the success of assisted reproduction methods in general, it is encouraging that the extensive biomedical and research use of cryopreserved, and banked, mouse spermatozoa (where ICSI is widely preferred as the fertilization method) do not appear to have produced the range of problems seen with fishes and amphibians [ 114 , 115 ].…”

Section: Art and Human Infertility Treatmentmentioning

confidence: 99%

Conservation Biology and Reproduction in a Time of Developmental Plasticity

Holt¹,

Comizzoli

2022

Biomolecules

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The objective of this review is to ask whether, and how, principles in conservation biology may need to be revisited in light of new knowledge about the power of epigenetics to alter developmental pathways. Importantly, conservation breeding programmes, used widely by zoological parks and aquariums, may appear in some cases to reduce fitness by decreasing animals’ abilities to cope when confronted with the ‘wild side’ of their natural habitats. Would less comfortable captive conditions lead to the selection of individuals that, despite being adapted to life in a captive environment, be better able to thrive if relocated to a more natural environment? While threatened populations may benefit from advanced reproductive technologies, these may actually induce undesirable epigenetic changes. Thus, there may be inherent risks to the health and welfare of offspring (as is suspected in humans). Advanced breeding technologies, especially those that aim to regenerate the rarest species using stem cell reprogramming and artificial gametes, may also lead to unwanted epigenetic modifications. Current knowledge is still incomplete, and therefore ethical decisions about novel breeding methods remain controversial and difficult to resolve.

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Development of assisted reproductive technologies forMus spretus†

Cited by 6 publications

References 49 publications

Naturally sterile Mus spretus hybrids are suitable for the generation of pseudopregnant embryo transfer recipients

Naturally sterile Mus spretus hybrids are suitable for the generation of pseudopregnant embryo transfer recipients

Derivation of embryonic stem cells from wild-derived mouse strains by nuclear transfer using peripheral blood cells

Conservation Biology and Reproduction in a Time of Developmental Plasticity

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