Obesity is a pervasive healthcare challenge impacting patient care and outcomes. Despite the crucial role of nurses in patient management, stigmatising attitudes towards obesity among nurses remain inadequately explored, particularly concerning female obese patients. Objective: This cross-sectional study aimed to assess nurses' stigmatising attitudes towards female obese patients and explore associated factors. Methods: The current study was conducted at Nishter Medical College and Hospital, Multan, from January 2022 to January 2023. It involved 100 nurses. A structured questionnaire gathered demographic data and utilised the Fat Phobia Scale (FPS) to gauge attitudes towards obesity. Data analysis employed descriptive and inferential statistics, including chi-square tests and regression analysis. Results: Predominantly female participants (70%) with a mean age of 32.5 years and an average experience of 8.2 years were included. The mean FPS score was 3.2, indicating moderate stigmatising attitudes towards obesity. Regression analysis revealed significant predictors of attitudes towards obesity, with years of experience and educational background playing key roles (p < 0.05). Conclusion: The findings underscore moderate levels of stigmatising attitudes towards female obese patients among nurses. Addressing these attitudes through targeted educational interventions and professional development initiatives holds promise for enhancing patient care and outcomes.