Development of Automated Headspace Gas Chromatography Determination of Dithiocarbamates in Plant Matrixes

Abstract

The determination of dithiocarbamates in plant matrixes is generally carried out by spectrophotometric (European Norm EN 12396-1, 1996) or gas chromatography headspace (European Norm EN 12396-2, 1999) methods. However, the former method presents a risk of carbon disulfide loss during hydrolysis and distillation and its sensitivity is low, whereas the latter method is time-consuming. In comparison to these European methods and in compliance with norm V03-110, we have developed an automated gas chromatography headspace method. This method offers a good level of accuracy and precision and is specific to the compound determined (CS(2)). The limit of detection is below 0.020 mg/kg and the limit of quantification is below 0.050 mg/kg. Moreover, the recovery rates are between 85 and 103% with RSD less than 20%. The automated headspace method has several advantages when compared to the spectrophotometric and manual headspace methods, including the reduction of reagents employed for extraction and a greater number of analyses achievable per day than the other methods (approximately 40 samples of food).

Stability of the dithiocarbamate pesticide maneb in tomato homogenates during cold storage and thermal processing

“…Homogenization of tomato samples upon receipt and immediate storage for a short period in a refrigerator at temperatures equal or below 5 C should have small impact on the residue levels recovered. The automatization and miniaturization of the EBDC analytical methodology applying the headspace gaschromatographic technique (Royer et al 2001), in particular, requires the use of homogenized laboratory samples. Furthermore, cold storage of juice in tomato processing plants should not result in any substantial reduction of maneb residues present nor give rise to increased levels of the toxicant ETU.…”

Persistence and distribution of pesticide residues in fresh agricultural food consumed in the province of Bologna

“…[23−25] Among them can be mentioned the spectrophotometric method for the determination of the dithiocarbamates [18,26] and the "multiresidual method". [16,17,27] The latter method has been employed in both the two different versions nowadays in use. [27] In the first version the homogenized samples have been extracted by acetone-methanol (1:1 v/v) mixture and the extract has been filtered.…”

Potential anticancer activity of Mn (II) complexes containing arginine dithiocarbamate ligand on MCF-7 breast cancer cell lines

“…Arginine is an amino acid that is needed by the body to run metabolism [ 7 ]. Dithiocarbamate also in the body has less toxicity [ 8 ]. These three reasons make the use of Mn (II) in combination with the arginine thiocarbamate ligand in cancer therapy to have very less side effects or complications.…”