Development of Basmati lines by the introgression of three bacterial blight resistant genes through marker-assisted breeding

Babar, Allah Ditta; Zaka, Abha; Naveed, Shahzad Amir; Ahmad, Niaz; Aslam, Kashif; Asif, Muhammad; Maqsood, Umer; Cruz, C. M. Vera; Arif, Muhammad

doi:10.1007/s10681-022-03013-z

Cited by 7 publications

(2 citation statements)

References 45 publications

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“…This has been used widely for different traits and crops, such as fruit weight and soluble solid content in tomato [13], flowering time in maize [17], grain weight in rice [18] or CMV resistance in melon [19,20]. In addition, it allows pyramiding of different QTLs and studying their interactions, as has been conducted for studying resistance to bacterial blight in rice [21] or resistance to Aphanomyces euteiches in pea [22]. ILs are developed by multiple backcrosses with the same parental line (the recurrent parent) and a last step of self-pollination to fix the heterozygous regions, providing a set of perpetual lines sharing most of the genome of the recurrent parent but having an introgression in a given chromosome from the other parental line (donor parent).…”

Section: Introductionmentioning

confidence: 99%

Fruit Morphology and Ripening-Related QTLs in a Newly Developed Introgression Line Collection of the Elite Varieties ‘Védrantais’ and ‘Piel de Sapo’

Domingo

Mayobre

Pereira

et al. 2022

Plants

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Melon is an economically important crop with widely diverse fruit morphology and ripening characteristics. Its diploid sequenced genome and multiple genomic tools make this species suitable to study the genetic architecture of fruit traits. With the development of this introgression line population of the elite varieties ‘Piel de Sapo’ and ‘Védrantais’, we present a powerful tool to study fruit morphology and ripening traits that can also facilitate characterization or pyramidation of QTLs in inodorous melon types. The population consists of 36 lines covering almost 98% of the melon genome, with an average of three introgressions per chromosome and segregating for multiple fruit traits: morphology, ripening and quality. High variability in fruit morphology was found within the population, with 24 QTLs affecting six different traits, confirming previously reported QTLs and two newly detected QTLs, FLQW5.1 and FWQW7.1. We detected 20 QTLs affecting fruit ripening traits, six of them reported for the first time, two affecting the timing of yellowing of the rind (EYELLQW1.1 and EYELLQW8.1) and four at the end of chromosome 8 affecting aroma, abscission and harvest date (EAROQW8.3, EALFQW8.3, ABSQW8.3 and HARQW8.3). We also confirmed the location of several QTLs, such as fruit-quality-related QTLs affecting rind and flesh appearance and flesh firmness.

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Section: Introductionmentioning

confidence: 99%

Fruit Morphology and Ripening-Related QTLs in a Newly Developed Introgression Line Collection of the Elite Varieties ‘Védrantais’ and ‘Piel de Sapo’

Domingo

Mayobre

Pereira

et al. 2022

Plants

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“…However, recently there have been reports of the breakdown of resistance governed by xa13 + Xa21 [39]. Subsequently, a major dominant BB resistance gene Xa38 with broad-spectrum resistance mapped on chromosome 4 [19] was incorporated into a series of elite rice varieties, viz., Pusa Basmati 1121, Super Basmati and Improved Samba Mahsuri [13,23,40]. The NILs carrying Xa38 were resistant to a greater number of races of Xoo as compared to the NILs carrying xa13 + Xa21, indicating a wider spectrum of resistance governed by the gene Xa38 [13].…”

Section: Discussionmentioning

confidence: 99%

Genetic Enhancement for Biotic Stress Resistance in Basmati Rice through Marker-Assisted Backcross Breeding

Singh,

Ellur

et al. 2023

IJMS

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Pusa Basmati 1509 (PB1509) is one of the major foreign-exchange-earning varieties of Basmati rice; it is semi-dwarf and early maturing with exceptional cooking quality and strong aroma. However, it is highly susceptible to various biotic stresses including bacterial blight and blast. Therefore, bacterial blight resistance genes, namely, xa13 + Xa21 and Xa38, and fungal blast resistance genes Pi9 + Pib and Pita were incorporated into the genetic background of recurrent parent (RP) PB1509 using donor parents, namely, Pusa Basmati 1718 (PB1718), Pusa 1927 (P1927), Pusa 1929 (P1929) and Tetep, respectively. Foreground selection was carried out with respective gene-linked markers, stringent phenotypic selection for recurrent parent phenotype, early generation background selection with Simple sequence repeat (SSR) markers, and background analysis at advanced generations with Rice Pan Genome Array comprising 80K SNPs. This has led to the development of Near isogenic lines (NILs), namely, Pusa 3037, Pusa 3054, Pusa 3060 and Pusa 3066 carrying genes xa13 + Xa21, Xa38, Pi9 + Pib and Pita with genomic similarity of 98.25%, 98.92%, 97.38% and 97.69%, respectively, as compared to the RP. Based on GGE-biplot analysis, Pusa 3037-1-44-3-164-20-249-2 carrying xa13 + Xa21, Pusa 3054-2-47-7-166-24-261-3 carrying Xa38, Pusa 3060-3-55-17-157-4-124-1 carrying Pi9 + Pib, and Pusa 3066-4-56-20-159-8-174-1 carrying Pita were identified to be relatively stable and better-performing individuals in the tested environments. Intercrossing between the best BC3F1s has led to the generation of Pusa 3122 (xa13 + Xa21 + Xa38), Pusa 3124 (Xa38 + Pi9 + Pib) and Pusa 3123 (Pi9 + Pib + Pita) with agronomy, grain and cooking quality parameters at par with PB1509. Cultivation of such improved varieties will help farmers reduce the cost of cultivation with decreased pesticide use and improve productivity with ensured safety to consumers.

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Pyramiding of blast and bacterial blight resistance genes in premium quality rice variety, BRRI dhan63 through marker-assisted breeding approach

Nihad,

Hasan,

Anik

et al. 2023

Euphytica

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Development of Basmati lines by the introgression of three bacterial blight resistant genes through marker-assisted breeding

Cited by 7 publications

References 45 publications

Fruit Morphology and Ripening-Related QTLs in a Newly Developed Introgression Line Collection of the Elite Varieties ‘Védrantais’ and ‘Piel de Sapo’

Fruit Morphology and Ripening-Related QTLs in a Newly Developed Introgression Line Collection of the Elite Varieties ‘Védrantais’ and ‘Piel de Sapo’

Genetic Enhancement for Biotic Stress Resistance in Basmati Rice through Marker-Assisted Backcross Breeding

Pyramiding of blast and bacterial blight resistance genes in premium quality rice variety, BRRI dhan63 through marker-assisted breeding approach

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