Development of biocatalysts for production of commodity chemicals from lignocellulosic biomass

Poly[3-hydroxybutyrate-co-3-hydroxyvalerate (3HV)] was produced in recombinant Escherichia coli LS5218 from the ruthenium-catalyzed cellulose hydrolysate and propionate. The strain was found to be resistant against 5-hydroxymethylfurfural (5-HMF), which is a major inhibitory byproduct generated in the cellulose hydrolysis reaction. The 3HV fraction was successfully regulated in the range of 5.6 -40 mol%.

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“…Furthermore, cellulosic biomass is inedible therefore its usage is not in direct competition with food or animal feeds production (2,3).…”

Section: Introductionmentioning

confidence: 99%

Polyhydroxyalkanoates production from cellulose hydrolysate in Escherichia coli LS5218 with superior resistance to 5-hydroxymethylfurfural

Nduko

Suzuki

Matsumoto

et al. 2012

Journal of Bioscience and Bioengineering

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“…2 These resources are widely used in stockbreeding, energy, biomaterials, chemicals, and other fields. [3][4][5] Due to the large amounts of production and waste of lignocellulose resources, they have not been used efficiently. 6 Based on the demonstrated potential, determining how to increase the utilization rate of cellulose in new and traditional fields is the subject of a great deal of research attention.…”

Section: Introductionmentioning

confidence: 99%

Construction of recombinant Yarrowia lipolytica and its application in bio-transformation of lignocellulose

et al. 2017

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Lignocellulose is a polysaccharide and an abundant biomass resource that widely exists in grains, beans, rice, and their by-products. Over 10 million tons of lignocellulose resources and processing products are produced every year in China. Three recombinant Y. lipolytica strains with cellulase (b-glucosidase, endoglucanase and cellobiohydrolase) were constructed. The enzymatic activities of these enzymes were 14.181 U/mL, 16.307 U/mL, and 17.391 U/mL, respectively. The whole cell cellulases were used for a stover bio-transformation. The celluloses in the stover were partly degraded by the cellulases, and the degradation products were transformed into single cell protein (SCP) by the Y. lipolytica cells. After 15 d of fermentation with the whole cell cellulases, the protein content of the maize stover and the rice straw reached 16.23% and 14.75%, which increased by 168.26% and 161.52% compared with the control, respectively. This study provides a new stage for the efficient utilization of stover in the feed industry.

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“…Bioconversion of lignocellulosic materials to biofuels and biomaterials has gained extensive research interest during the last few decades due to the feasibility of this sustainable and eco-friendly process [ADSUL et al, 2011, BOSTAN et al, 2013, BUTNARIU and CAUNII, 2013 . It is a promising feedstock for the bio-processing industries.…”

Section: Introductionmentioning

confidence: 99%

Investigations on Microbial Fermentation of Hemicellulose Hydrolysate for Xylitol Production

Bhattacharya¹,

Sadhukhan²,

Ganguly³

et al. 2016

bjbabe

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Abstract.A crude trial on microbial fermentation of dilute acid treated hemicellulose hydrolysate of water hyacinth (Eichhornia crassipes) biomass was performed in batch culture in a BOD rotary shaker using four different species as Candida shehatae, Pichia stipitis, Candida tropicalis and Debaromyces hansenii under various parameters to check the optimal xylitol production. The hydrolysate was concentrated using a vacuum evaporator at 70  C and detoxified using activated charcoal (5 %) which efficiently removed 83 % of inhibitory phenolic biproducts. Effect of nutrients concentration, pH (4.5, 5.5, 6.5), temperature (20, 30, 40  C) and agitation speed (150, 200, 250 rpm) on growth of different yeast and xylitol production was studied and compared. The optimal xylitol yield was found to be 0.65 g xylitol/g of xylose from Candida tropicalis at 30  C, pH 5.5 with an agitation of 200 rpm, 2.5 g/L ammonium sulphate as a nutrient and 0.615 xylitol/g of xylose from Pichia stipitis respectively after 72 h of incubation.

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Development of biocatalysts for production of commodity chemicals from lignocellulosic biomass

Cited by 179 publications

References 83 publications

Polyhydroxyalkanoates production from cellulose hydrolysate in Escherichia coli LS5218 with superior resistance to 5-hydroxymethylfurfural

Polyhydroxyalkanoates production from cellulose hydrolysate in Escherichia coli LS5218 with superior resistance to 5-hydroxymethylfurfural

Construction of recombinant Yarrowia lipolytica and its application in bio-transformation of lignocellulose

Investigations on Microbial Fermentation of Hemicellulose Hydrolysate for Xylitol Production

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