Development of Biochar-Based Whole-Cell Biocatalysts for the Production of <scp>l</scp>-Tryptophan and <scp>l</scp>-Phenylalanine

Su, Yu Ping; Li, Qingchen; Gu, Shitan; Liu, Qinghao; He, Wei; Huang, Jianzhong; Wu, Wei; Qi, Feng

doi:10.1021/acssuschemeng.3c01160

Cited by 3 publications

(1 citation statement)

References 43 publications

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“…l -Trp must be ingested from the diet and is widely used in food, animal feed, chemical, and pharmaceutical industries . Finally, the plasmid p6LRPW326, harboring the trp operon, , was expressed in the metabolically engineered E. coli strains with integrated formate assimilation and enhanced acetate assimilation for de novo biosynthesis of l -Trp from lignocellulosic hydrolysate as the sole carbon source.…”

Section: Introductionmentioning

confidence: 99%

Integrated and Enhanced Coassimilation of Formate and Acetate for Efficient Utilization of Lignocellulosic Hydrolysate

Jin,

Bao,

Yang

et al. 2024

ACS Sustainable Chem. Eng.

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Hydrolysis of lignocellulosic biomass is widely recognized as the most commonly used pretreatment method. The presence of formate and acetate, which constitute a notable proportion of inhibitory compounds in lignocellulosic hydrolysate, hinders their efficient utilization as a carbon source. Herein, we performed metabolic engineering on E. coli strains by introducing a heterologous RuMP pathway and increasing the intracellular pools of the essential cofactors NADPH and ATP to achieve formate assimilation and enhance acetate assimilation, respectively. 13C-isotope analysis indicated that formate assimilation occurred through the exogenous heterologous RuMP pathway rather than through the endogenous formate-fixing mechanisms. Enhancement of acetate assimilation can be obtained by genetic modification to increase the intracellular support of NADPH and ATP. Furthermore, we incorporated the ability for formate assimilation and improved acetate assimilation into the E. coli strains that have been engineered for L-tryptophan biosynthesis, resulting in the recombinant strain FATrp16. When lignocellulosic hydrolysate was utilized as the sole carbon source, FATrp16 showed a marked improvement in the consumption of formate and acetate, along with the high production of l-Trp (0.38 g/gDCW) in flask culture. This study presents an integrated approach to facilitate the efficient utilization of lignocellulosic hydrolysate.

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Section: Introductionmentioning

confidence: 99%

Integrated and Enhanced Coassimilation of Formate and Acetate for Efficient Utilization of Lignocellulosic Hydrolysate

Jin,

Bao,

Yang

et al. 2024

ACS Sustainable Chem. Eng.

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Enhanced adsorption of polystyrene nanoplastics by cetyltrimethylammonium bromide surface-modified magnetic rice straw biochar: Efficient performance and adsorption mechanisms

Xing,

Zhang,

Niu

et al. 2024

Separation and Purification Technology

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Biodiesel Production from Waste Cooking Oil Using Recombinant Escherichia coli Cells Immobilized into Fe3O4–Chitosan Magnetic Microspheres

Zhao,

Han,

Zhou

et al. 2024

Molecules

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Developing reusable and easy-to-operate biocatalysts is of significant interest in biodiesel production. Here, magnetic whole-cell catalysts constructed through immobilizing recombinant Escherichia coli cells (containing MAS1 lipase) into Fe3O4–chitosan magnetic microspheres (termed MWCC@MAS1) were used for fatty acid methyl ester (FAME) production from waste cooking oil (WCO). During the preparation process of immobilized cells, the effects of chitosan concentration and cell concentration on their activity and activity recovery were investigated. Optimal immobilization was achieved with 3% (w/v) chitosan solution and 10 mg wet cell/mL cell suspension. Magnetic immobilization endowed the whole-cell catalysts with superparamagnetism and improved their methanol tolerance, enhancing the recyclability of the biocatalysts. Additionally, we studied the effects of catalyst loading, water content, methanol content, and reaction temperature on FAME yield, optimizing these parameters using response surface methodology and Box–Behnken design. An experimental FAME yield of 89.19% was gained under the optimized conditions (3.9 wt% catalyst loading, 22.3% (v/w) water content, 23.0% (v/w) methanol content, and 32 °C) for 48 h. MWCC@MAS1 demonstrated superior recyclability compared to its whole-cell form, maintaining about 86% of its initial productivity after 10 cycles, whereas the whole-cell form lost nearly half after just five cycles. These results suggest that MWCC@MAS1 has great potential for the industrial production of biodiesel.

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Development of Biochar-Based Whole-Cell Biocatalysts for the Production of l-Tryptophan and l-Phenylalanine

Cited by 3 publications

References 43 publications

Integrated and Enhanced Coassimilation of Formate and Acetate for Efficient Utilization of Lignocellulosic Hydrolysate

Integrated and Enhanced Coassimilation of Formate and Acetate for Efficient Utilization of Lignocellulosic Hydrolysate

Enhanced adsorption of polystyrene nanoplastics by cetyltrimethylammonium bromide surface-modified magnetic rice straw biochar: Efficient performance and adsorption mechanisms

Biodiesel Production from Waste Cooking Oil Using Recombinant Escherichia coli Cells Immobilized into Fe3O4–Chitosan Magnetic Microspheres

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