Development of Biosensing Devices and Systems Using Micro/Nanoelectrodes

Matsue, Tomokazu

doi:10.1246/bcsj.20110249

Cited by 12 publications

(10 citation statements)

References 74 publications

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“…[137][138][139] Advantages include low ohmic drops, fast mass transport, capabilities for localized, highthroughput measurements, and potential for multiplexing. 140,141 A large body of work may be found on the development of micro-/nanoelectrode biosensing devices, particularly for neuroelectronic interfacing. 10,142,143 In this section, we highlight electrodes designed to probe electronic communication in bioelectrochemical devices, as well as new electrode materials and architectures for intracellular recording.…”

Section: Micro-and Nanoelectrodesmentioning

confidence: 99%

Modification of Abiotic–Biotic Interfaces with Small Molecules and Nanomaterials for Improved Bioelectronics

Catania

Bazan

2013

Chem. Mater.

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Low efficiency of charge extraction (and conversely charge injection) across biotic-abiotic interfaces constitutes an obstacle to the integration of biological and electronic systems in high-performance bioelectronic devices. Advances in the promotion of charge transport across these typically non-conductive interfaces will have far-reaching implications in important applications such as alternative energy generation, bioelectrosynthesis, diagnostics, and environmental monitoring. This review highlights the use of synthetic materials to improve electrical interfacing between biological systems and electrodes, focusing specifically on whole cell bioelectrochemical systems. By taking advantage of a rich variety of materials chemistry and synthetic methodologies, significant improvements to the facilitation of charge transport across abiotic-biotic interfaces have been realized. The modifications of the bioelectronic interfaces presented herein include the use of organic small molecules, semiconducting and redox active polymers, inorganic nanoparticles, carbon nanotubes, graphene, hybrid organic-inorganic systems, and micro-/nanoelectrodes. However, design rules to guide material selection and choices regarding device architecture remain ambiguous. Establishment of a clearer understanding of bioelectronic charge transfer phenomena, their constituent pathways, and means of stimulating or selecting for different pathways is still work in progress. As such, great opportunities exist for materials scientists to contribute to these topics through design and implementation.

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Section: Micro-and Nanoelectrodesmentioning

confidence: 99%

Modification of Abiotic–Biotic Interfaces with Small Molecules and Nanomaterials for Improved Bioelectronics

Catania

Bazan

2013

Chem. Mater.

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“…Scanning electrochemical microscopy (SECM) allows for the noninvasive characterization of mammalian embryos and cellular aggregates in order to measure local oxygen concentrations. [22][23][24][25][26][27][28][29] SECM has been applied for assessing the quality of embryos and cellular aggregates that are used for embryo transfer and cellular transplantation. [30][31][32][33] The goal of our research was to find useful marker genes to evaluate the status of differentiation of mEBs that corresponds to changes in respiratory activity.…”

Section: Introductionmentioning

confidence: 99%

Noninvasive measurement of respiratory activity of mouse embryoid bodies and its correlation with mRNA levels of undifferentiation/differentiation markers

et al. 2013

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Mouse embryoid bodies (mEBs) were evaluated in detail on the basis of respiratory activity and high-throughput quantitative reverse transcription-PCR (RT-qPCR) analysis. The hanging drop culture method was applied to prepare various sizes of mEBs ranging from 100 to 250 μm in radius by causing the aggregation of embryonic cells. The respiratory activity of individual mEBs was noninvasively measured using scanning electrochemical microscopy in a cone-shaped microwell. For gene expression analysis, we used 48.48 Dynamic Array chips (Fluidigm) integrating microfluidic circuits, which allowed high-throughput qPCR analysis in parallel. The respiratory activity of the mEBs that were cultured for 1 to 6 days could predict the mRNA levels of undifferentiation and differentiation markers. However, the sizes of the mEBs could also predict the gene expression of the undifferentiation/differentiation markers because the radii of the mEBs increased by more than 2-fold after incubation in hanging drop culture for 6 days. Next, mEBs with identical sample sizes were evaluated for respiratory activity and gene expression. For mEBs cultured at 1500 cells per droplet for 3 days, the respiratory activity was negatively correlated with the mRNA levels of pluripotent markers such as Nanog and Sox2. Many differentiation markers were positively correlated with the respiratory activity. However, there was no significant difference in respiration activity between the beating and nonbeating samples on day 3. Finally, principal component analysis (PCA) confirmed the relationship between respiratory activity and the mRNA levels of undifferentiation/differentiation markers.

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“…Microelectrodes have been applied to in vivo, intra-and extracellular electrochemistry since the 1970s. [1][2][3] Two experimental approaches have been used for amperometric measurements on biological cells: stylus-type microelectrode probes and electrodeintegrated microdevices based on microelectronic industry.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Biosensing System for Single Cells, Cellular Aggregates and Microenvironments

Shiku

2018

ANAL. SCI.

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Applications of electrochemical biosensing for surveying intact cells and tissues have been focus of attention. Two experimental approaches have been used when performing amperometric measurements on biological cells, the stylustype microelectrode probes and the electrode-integrated microdevices based on lithographic technologies. For the probe scanning approach, various types of microsensors were developed to monitor localized physical or chemical natures at a variety of surfaces in situ under wet conditions. Scanning electrochemical microscopy (SECM) has been applied for monitoring local oxygen, enzyme activity, and collection of transcripts. For the non-scanning type of approach, electrode array devices allow very rapid response, parallel monitoring, and multi-analyte assay. Sveral topics of on-chip-culture system were introduced especially concerning on gene expression monitoring by reporter system and reconstruction of in vivo-like nature by controlling microenvironments. Electrochemical reporter assay has been demonstrated to monitor the gene expression process of the gene-modified cultured cells. Long-term monitoring of cellular function of spheroids and three dimensionally-cultured cells were carried out by controlling microenvironments on the cellular chip.

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Development of Biosensing Devices and Systems Using Micro/Nanoelectrodes

Cited by 12 publications

References 74 publications

Modification of Abiotic–Biotic Interfaces with Small Molecules and Nanomaterials for Improved Bioelectronics

Modification of Abiotic–Biotic Interfaces with Small Molecules and Nanomaterials for Improved Bioelectronics

Noninvasive measurement of respiratory activity of mouse embryoid bodies and its correlation with mRNA levels of undifferentiation/differentiation markers

Electrochemical Biosensing System for Single Cells, Cellular Aggregates and Microenvironments

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