Development of Biotechnology for Anthrax Bacteriophages Reproduction, and Comparative Analysis of Their Properties Stability and Diagnostic Value

Abstract

Carried out has been complex comparative analysis of the production and diagnostic properties of such anthrax bacteriophages as Gamma A-26, Fah-RRIVV&M (Russian Research Institute of Veterinary Virology and Microbiology), R/D-Ph-6, BA-9, K RRIEV (Russian Research Institute of Experimental Veterinary), Saratov, and 186. It is revealed that all of the bacteriophages have the properties necessary for using them for preparation manufacturing purposes: they readily proliferate in liquid cultures of avirulent Bacillus anthracis strain-STI, and retain sufficient specific activity if stored at the optimum temperatures or, when exposed to short-term temperature excursions, within a period of two years. Most clearly expressed are the differences in diagnostic properties of the bacteriophages-specific activity and specificity. Put forward are the groups of bacteriophages that are the most relevant for identification or phage-typing of B. anthracis strains.