Development of biotin-streptavidin amplified peptide antibody-based ELISA for quantification of αS1-casein in goat’s milk

Mohsin, Aliah Zannierah; Selamat, Jinap; Hussin, Anis Shobirin Meor; Ismail, Intan Hakimah; Azri, Farah Asilah; Mustafa, Radhiatul Raehan; Mansor, Muzammeer

doi:10.1016/j.foodcont.2022.109263

Cited by 6 publications

(8 citation statements)

References 34 publications

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“…However, in our study, the utilisation of both heating with denaturing agents showed a diminished signal-to-noise ratio when attempting to identify the entire goat casein. This could be attributed to the specific detection of goat a s1 -casein by Nter-IgG and Cter-IgG, as previously reported by Mohsin et al (2023).…”

Section: Blocking Reagentssupporting

confidence: 81%

“…According to Mohsin et al (2023), there was no significant difference (P > 0.05) observed in the incubation duration of 2 and 3 h using 2.5% BSA. Therefore, a blocking period of 2 h was selected to maintain the efficiency of the system.…”

Section: Blocking Reagentsmentioning

confidence: 79%

“…N‐terminal and C‐terminal IgGs were produced from α s1 ‐casein peptides from goat's milk developed in rabbits, as previously reported by Mohsin et al . (2023). Rabbit polyclonal casein antibody with biotin‐conjugate was purchased from Bioss (Woburn, MA), and rabbit polyclonal antibody was obtained from GeneTex (Irvine, CA).…”

Section: Methodsmentioning

confidence: 99%

“…In contrast to another study that was conducted on the specific composition of goat's milk casein, α s1 (Mohsin et al ., 2023), this study aims to examine and enhance the usability of different types of ELISA systems, Fig. 1a–c, for the detection of whole casein composition from goat's, also known as caprine's milk.…”

Section: Introductionmentioning

confidence: 99%

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Comparative detection of goat's milk casein through various enzyme‐linked immunosorbent assay (ELISA) approaches using caprine alpha‐_S1 casein peptides and bovine casein polyclonal antibodies

Samsudin,

Sukor,

Mohsin

et al. 2024

Int J of Food Sci Tech

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SummaryCasein causes allergic reactions in cow's milk protein allergy (CMPA) individuals. Casein can cross‐react with cows and goats. Hence, a rapid and highly specific technique is required for its detection. The study aims to evaluate and optimise multiple Enzyme‐Linked Immunosorbent Assay (ELISA) approaches for the detection of casein isolated from goat's milk. The study utilised and optimised various ELISA approaches, including direct, indirect and sandwich with various antibodies. Several parameters of ELISA including pre‐treatments of casein, blocking reagent, concentrations of antibodies and background signal were evaluated. The results showed that sandwich ELISA revealed a good signal‐to‐noise ratio at 1 mg mL−1. Indirect ELISA showed a higher signal‐to‐noise ratio without any heat treatment in comparison to the direct ELISA despite utilising an equivalent concentration (i.e., 10 μg mL−1) of goat's milk casein. The findings provide a theoretical foundation for obtaining a high signal‐to‐noise ratio for antigenicity studies of goat's milk casein to minimise its immunological response.

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Section: Blocking Reagentssupporting

confidence: 81%

Section: Blocking Reagentsmentioning

confidence: 79%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative detection of goat's milk casein through various enzyme‐linked immunosorbent assay (ELISA) approaches using caprine alpha‐_S1 casein peptides and bovine casein polyclonal antibodies

Samsudin,

Sukor,

Mohsin

et al. 2024

Int J of Food Sci Tech

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“…Biotin and streptavidin have high specificity and strong affinity of interaction ( K d = 10 –15 M). SA–Bio system has been developed as a signal amplification method to improve the sensitivity of all kinds of biosensors. − Here, SA–Bio was first introduced into sandwich FLISA to further improve the sensitivity based on InP-based QDs. The principle of SA–Bio FLISA for CRP detection is illustrated in Scheme .…”

Section: Resultsmentioning

confidence: 99%

Highly Sensitive, Stable InP Quantum Dot Fluorescent Probes for Quantitative Immunoassay Through Nanostructure Tailoring and Biotin–Streptavidin Coupling

Zhang,

Xu,

Zhang

et al. 2024

Inorg. Chem.

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Nontoxic, highly sensitive InP quantum dot (QD) fluorescent immunoassay probes are promising biomedical detection modalities due to their unique properties. However, InP-based QDs are prone to surface oxidation, and the stability of InP QD-based probes in biocompatible environments remains a crucial challenge. Although the thick shell can provide some protection during the phase transfer process of hydrophobic QDs, the photoluminescence quantum yield (PLQY) is generally decreased because of the contradiction between lattice stress relaxation and thick shell growth. Herein, we developed thick-shell InP-based core/shell QDs by inserting a ZnSeS alloy layer. The ternary ZnSeS intermediate shell could effectively facilitate lattice stress relaxation and passivate the defect states. The synthesized InP/ZnSe/ZnSeS/ZnS core/alloy shell/shell QDs (CAS-InP QDs) with nanostructure tailoring revealed a larger size, high PLQY (90%), and high optical stability. After amphiphilic polymer encapsulation, the aqueous CAS-InP QDs presented almost constant fluorescence attenuation and stable PL intensity under different temperatures, UV radiation, and pH solutions. The CAS-InP QDs were excellent labels of the fluorescence-linked immunosorbent assay (FLISA) for detecting C-reactive protein (CRP). The biotin–streptavidin (Bio–SA) system was first introduced in the FLISA to further improve the sensitivity, and the CAS-InP QDs-based SA–Bio sandwich FLISA realized the detection of CRP with an impressive limit of detection (LOD) of 0.83 ng/mL. It is believed that the stable and sensitive InP QD fluorescent probes will drive the rapid development of future eco-friendly, cost-effective, and sensitive in vitro diagnostic kits.

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Highly sensitive digital detection of SARS-CoV-2 nucleocapsid protein through single-molecule counting

Dou,

Zhang,

Liu

et al. 2023

Anal Bioanal Chem

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Development of biotin-streptavidin amplified peptide antibody-based ELISA for quantification of αS1-casein in goat’s milk

Cited by 6 publications

References 34 publications

Comparative detection of goat's milk casein through various enzyme‐linked immunosorbent assay (ELISA) approaches using caprine alpha‐_S1 casein peptides and bovine casein polyclonal antibodies

Comparative detection of goat's milk casein through various enzyme‐linked immunosorbent assay (ELISA) approaches using caprine alpha‐_S1 casein peptides and bovine casein polyclonal antibodies

Highly Sensitive, Stable InP Quantum Dot Fluorescent Probes for Quantitative Immunoassay Through Nanostructure Tailoring and Biotin–Streptavidin Coupling

Highly sensitive digital detection of SARS-CoV-2 nucleocapsid protein through single-molecule counting

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Development of biotin-streptavidin amplified peptide antibody-based ELISA for quantification of αS1-casein in goat’s milk

Cited by 6 publications

References 34 publications

Comparative detection of goat's milk casein through various enzyme‐linked immunosorbent assay (ELISA) approaches using caprine alpha‐S1 casein peptides and bovine casein polyclonal antibodies

Comparative detection of goat's milk casein through various enzyme‐linked immunosorbent assay (ELISA) approaches using caprine alpha‐S1 casein peptides and bovine casein polyclonal antibodies

Highly Sensitive, Stable InP Quantum Dot Fluorescent Probes for Quantitative Immunoassay Through Nanostructure Tailoring and Biotin–Streptavidin Coupling

Highly sensitive digital detection of SARS-CoV-2 nucleocapsid protein through single-molecule counting

Contact Info

Product

Resources

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Comparative detection of goat's milk casein through various enzyme‐linked immunosorbent assay (ELISA) approaches using caprine alpha‐_S1 casein peptides and bovine casein polyclonal antibodies

Comparative detection of goat's milk casein through various enzyme‐linked immunosorbent assay (ELISA) approaches using caprine alpha‐_S1 casein peptides and bovine casein polyclonal antibodies