Development of boronate affinity-based magnetic composites in biological analysis: Advances and future prospects

Lin, Hong; Chen, Xiangfeng; Tian, Jiaojiao; Pavase, Tushar Ramesh; Wang, Ruiqian; Sui, Jianxin; Cao, Limin

doi:10.1016/j.trac.2020.115952

Cited by 31 publications

(22 citation statements)

References 141 publications

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“… 23 , 24 The performances of boronic acid based adsorbents are dependent on both the affinity ligands and the supporting materials. 25 Nanomaterial-supported boronic acid ligands exhibit several significant advantages including fast adsorption/desorption kinetics, pH-controlled capture/release process, and broad-spectrum affinity. 26 Because of its unique structural characteristics, DFNS can add one additional selectivity defined by molecular sizes in addition to the well-known boronic acid– cis -diol interactions.…”

Section: Resultsmentioning

confidence: 99%

Boronic Acid Functionalized Nanosilica for Binding Guest Molecules

Xue

Gong

2021

ACS Appl. Nano Mater.

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Dendritic fibrous nanosilica (DFNS) has very high surface area and well-defined nanochannels; therefore, it is very useful as supporting material for numerous applications including catalysis, sensing, and bioseparation. Due to the highly restricted space, addition of molecular ligands to DFNS is very challenging. This work studies how ligand conjugation in nanoscale pores in DFNS can be achieved through copper-catalyzed click reaction, using an optional, in situ synthesized, temperature-responsive polymer intermediate. A clickable boronic acid is used as a model to investigate the ligand immobilization and the molecular binding characteristics of the functionalized DFNS. The morphology, composition, nanoscale pores, and specific surface area of the boronic acid functionalized nanosilica were characterized by electron microscopy, thermogravimetric and elemental analysis, Fourier transform infrared spectroscopy, and nitrogen adsorption–desorption measurements. The numbers of boronic acid molecules on the modified DFNS with and without the polymer were determined to be 0.08 and 0.68 mmol of ligand/g of DFNS, respectively. We also studied the binding of small cis -diol molecules in the nanoscale pores of DFNS. The boronic acid modified DFNS with the polymer intermediate exhibits higher binding capacity for Alizarin Red S and nicotinamide adenine dinucleotide than the polymer-free DFNS. The two types of boronic acid modified DFNS can bind small cis -diol molecules in the presence of large glycoproteins, due in large part to the effect of size exclusion provided by the nanochannels in the DFNS.

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Section: Resultsmentioning

confidence: 99%

Boronic Acid Functionalized Nanosilica for Binding Guest Molecules

Xue

Gong

2021

ACS Appl. Nano Mater.

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“…For example, it was reported that the interphase region, locating between polymer matrix and filled particles, facilitates the establishment of conductive networks. [23][24][25] Besides, the interphase region benefits shifting the percolation threshold to a lower concentration of filled particles, which undoubtedly affects the electrical conductivity of the nanocomposite. 26,27 Figure 1 displays a schematic illustration of nanoparticles which are surrounded with interphase regions.…”

Section: Introductionmentioning

confidence: 99%

Effect of interfacial properties of filled carbon black nanoparticles on the conductivity of nanocomposite

Jian

2021

J of Applied Polymer Sci

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In this study, an optimized model is proposed for predicting the effective conductivity of nanocomposites, which contains conductive spherical fillers above the percolation threshold. The influence of filler properties, interphase regions, filler concentrations, elemental cell sizes as well as contact area on the effective conductivity of nanocomposites was investigated and discussed in detail.The developed model is verified by using the experimental data reported in the literatures, and it is found that the prediction results are fitted well with the experimental results at different particle concentrations. In addition, high levels of filler conductivity, particle concentration, and elemental cell size led to a good conductivity. The maximum conductivity of nanocomposite with σ eff = 1,500,000 S/m, V f = 0.07, r = 10 À4 nm, and D = 1250 nm is obtained at R i = 40 nm and d = 10 nm. Furthermore, the conductivity of nanocomposite is not sensitive to interphase thickness, when d is larger than 25 nm.

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“…[32] reviewed the pros and cons of different chromatographic methods for glycoprotein and glycopeptide enrichment. The reviews published from 2016 onwards have focused on the application of different stationary phase materials (metal‐organic frameworks [MOFs] [33–36], covalent organic frameworks [37], magnetic nanoparticles [35, 38–40], or monoliths [41]) for biomolecule enrichment including glycoproteins and glycopeptides. Qing et al.…”

Section: Introductionmentioning

confidence: 99%

A review on recent advances in the enrichment of glycopeptides and glycoproteins by liquid chromatographic methods: 2016–Present

Kumari

Tetala

2021

Electrophoresis

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Among various protein post‐translational modifications (PTMs), glycosylation has received special attention due to its immense role in molecular interactions, cellular signal transduction, immune response, etc. Aberration in glycan moieties of a glycoprotein is associated with cancer, diabetes, and bacterial and viral infections. In biofluids (plasma, saliva, urine, milk, etc.), glycoproteins are low in abundance and are masked by the presence of high abundant proteins. Hence, prior to their identification using mass spectrometry methods, liquid chromatography (LC)‐based approaches were widely used. A general enrichment strategy involves a protein digestion step, followed by LC‐based enrichment and desorption of glycopeptides, and enzymatic excision of the glycans. The focus of this review article is to highlight the articles published since 2016 that dealt with different LC‐based approaches for glycopeptide and glycoprotein enrichment. The preparation of stationary phases, their surface activation, and ligand immobilization strategies have been discussed in detail. Finally, the major developments and future trends in the field have been summarized.

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Development of boronate affinity-based magnetic composites in biological analysis: Advances and future prospects

Cited by 31 publications

References 141 publications

Boronic Acid Functionalized Nanosilica for Binding Guest Molecules

Boronic Acid Functionalized Nanosilica for Binding Guest Molecules

Effect of interfacial properties of filled carbon black nanoparticles on the conductivity of nanocomposite

A review on recent advances in the enrichment of glycopeptides and glycoproteins by liquid chromatographic methods: 2016–Present

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