2011
DOI: 10.2164/jandrol.110.010322
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Development of Bovine Fetal Testis Tissue After Ectopic Xenografting in Mice

Abstract: : Testis tissue xenografting represents a versatile model to study testis biology, and to preserve fertility in immature animals. To evaluate whether bovine fetal testes can mature when grafted into mouse hosts, small fragments of testes from midgestation (125 to 145 days of gestation) bovine fetuses were grafted ectopically into immunodeficient castrated male mice. At grafting, donor tissue displayed the typical seminiferous cords composed of gonocytes and primitive Sertoli cells. At 5 or 10 months after gra… Show more

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Cited by 13 publications
(12 citation statements)
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“…In an earlier report, elevated testosterone level in bovines has shown increased spermatogenesis and larger number of SCs per tubule cross section accompanied with increased concentrations of spermatozoa in ejaculate (Chandolia et al 1997). Immunolocalization of PCNA has been shown to be a validated approach for the study of cell proliferation in developing testes (Rathi et al 2008, Rodriguez-Sosa et al 2011. PCNA-stained SC number was similar in grafts recovered from castrated or intact recipients at a given collection time.…”
Section: Discussionmentioning
confidence: 88%
See 1 more Smart Citation
“…In an earlier report, elevated testosterone level in bovines has shown increased spermatogenesis and larger number of SCs per tubule cross section accompanied with increased concentrations of spermatozoa in ejaculate (Chandolia et al 1997). Immunolocalization of PCNA has been shown to be a validated approach for the study of cell proliferation in developing testes (Rathi et al 2008, Rodriguez-Sosa et al 2011. PCNA-stained SC number was similar in grafts recovered from castrated or intact recipients at a given collection time.…”
Section: Discussionmentioning
confidence: 88%
“…This finding was further corroborated by low serum testosterone levels in castrated recipients at both collection times. Although xenografts from fetal and neonatal bovine testes in mouse recipients have been shown to maintain serum testosterone level (Rathi et al 2005, Rodriguez-Sosa et al 2011, equine testis xenografts produced less bioactive testosterone (Rathi et al 2006) as indicated by the seminal gland weight. Similarly, castrated mice xenografted with infant monkey testis tissue showed lower seminal vesicle weight in gonadotropin-untreated mice compared with the treated one (Rathi et al 2008).…”
Section: Discussionmentioning
confidence: 99%
“…Previous studies on xenografting fetal testicular tissue have focused mainly on humans (Povlsen et al 1974; Skakkebæk et al 1974; Yu et al 2006; Mitchell et al 2010), whereas only one study has reported work on bovine fetal testis tissue (Rodriguez-Sosa et al 2011). Therefore, the present study is the first to use fetal testicular tissue from endangered species as donor material.…”
Section: Discussionmentioning
confidence: 99%
“…Therefore, the present study is the first to use fetal testicular tissue from endangered species as donor material. Human and bovine fresh fetal tissue survived after being xenografted into nude mice, and human tissue showed normal structure and function (Yu et al 2006; Mitchell et al 2010; Rodriguez-Sosa et al 2011). However, in humans, differentiated germ cells were not found, although perhaps the recovery time (maximum 19 weeks) was not sufficient to reach the onset of spermatogenesis.…”
Section: Discussionmentioning
confidence: 99%
“…Seminal vesicles were weighed as an indicator of T secretion by grafts (24). Pieces of testicular tissue from donors and the recovered grafts were fixed in Bouin’s solution after dissection and processed for histology as described previously (25). …”
Section: Methodsmentioning
confidence: 99%