Development of cellulases to improve enzymatic hydrolysis of lignocellulosic biomass

Quinlan, R. Jason; Teter, Sarah; Xu, Feng

doi:10.1533/9781845699611.2.178

Cited by 6 publications

(4 citation statements)

References 95 publications

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“…Thus, an increase in endoglucanase dosage above the saturation point might not be effective (Gourlay et al 2018). On the other hand, exoglucanases, such as cellobiohydrolases are known to attack the cellulose chains from either end, releasing cellobiose units (Quinlan et al 2010). The presence of lignin and hemicelluloses has been found to reduce the extent of cellulase activity due to inaccessibility of cellulose for the enzyme to bind upon (Igarashi et al 2011;Laureano-Perez et al 2005;Mosier et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Rheological behavior of high consistency enzymatically fibrillated cellulose suspensions

et al. 2021

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High-consistency processing of fibrillated cellulose materials is attractive for commercial applications due to potential for lowered production costs, energy savings and easier logistics. The current work investigated structure–property relationships of fibrillated cellulose suspensions produced at 20% consistency using VTT HefCel (High-consistency enzymatic fibrillation of cellulose) technology. Morphological examination of the fibrillated materials revealed that enzymatic action on the cellulose substrates was not a direct function of enzyme dosage but rather was dependent on the raw material composition. Furthermore, shear viscosity of the HefCel suspensions was found to decrease with increasing enzyme dosage while the water retention increased. The shear viscosity followed power law relationship with the power law index varying in the range 0.11–0.73. The shear-thinning behavior decreased with increasing consistency. Moreover, suspension viscosity ($$\upmu$$ μ ) was found to be highly dependent on the consistency ($$\mathrm{c})$$ c ) as $$\upmu \sim {\mathrm{c}}^{\mathrm{m}}$$ μ ∼ c m , with $$\mathrm{m}$$ m ranging from 2.75 to 4.31 for different samples. Yield stress (τy) of the HefCel suspensions was measured at 7 and 10% consistencies. The performance of the fibrillated cellulose grades in a typical application was demonstrated by casting films, which were characterized for their mechanical properties. Graphic abstract

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Section: Introductionmentioning

confidence: 99%

Rheological behavior of high consistency enzymatically fibrillated cellulose suspensions

et al. 2021

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“…Although there are different commercial cellulases obtained from fungal strains, their enzymatic activity is limited to volumetric activity, but not to its specificity to the substrate. This volumetric activity implies an increase in production costs because large amounts of these cellulases are required to achieve complete hydrolysis of polymeric matrices susceptible to enzymatic attack ( Quinlan, Teter & Xu, 2010 ). Although high values of cellulolytic activity were reached under the evaluated conditions, the fermentation time necessary for Streptomyces sp.…”

Section: Discussionmentioning

confidence: 99%

Systematic bioprospection for cellulolytic actinomycetes in the Chihuahuan Desert: isolation and enzymatic profiling

Escudero-Agudelo,

Martínez-Villalobos,

Arocha-Garza

et al. 2023

PeerJ

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The quest for microbial cellulases has intensified as a response to global challenges in biofuel production. The efficient deconstruction of lignocellulosic biomass holds promise for generating valuable products in various industries such as food, textile, and detergents. This article presents a systematic bioprospection aimed at isolating actinomycetes with exceptional cellulose deconstruction capabilities. Our methodology explored the biodiverse oligotrophic region of Cuatro Cienegas, Coahuila, within the Chihuahuan Desert. Among the evaluated actinomycetes collection, 78% exhibited cellulolytic activity. Through a meticulous screening process based on enzymatic index evaluation, we identified a highly cellulolytic Streptomyces strain for further investigation. Submerged fermentation of this strain revealed an endoglucanase enzymatic activity of 149 U/mg. Genomic analysis of strain Streptomyces sp. STCH565-A revealed unique configurations of carbohydrate-active enzyme (CAZyme) genes, underscoring its potential for lignocellulosic bioconversion applications. These findings not only highlight the significance of the Chihuahuan Desert as a rich source of cellulolytic microorganisms but also offer insights into the systematic exploration and selection of high-performing cellulolytic microorganisms for application in diverse environmental contexts. In conclusion, our bioprospecting study lays a foundation for harnessing the cellulolytic potential of actinomycetes from the Chihuahuan Desert, with implications for advancing cellulose deconstruction processes in various industries. The findings can serve as a blueprint for future bioprospecting efforts in different regions, facilitating the targeted discovery of microorganisms with exceptional cellulosic deconstruction capabilities.

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“…[96] The second method is specific to cellulose and uses the cellulose-binding module (CBM), a distinct unit found in cellulase with unique structure, function, and ability to bind to carbohydrates. [109] In the conventional method, one of the binding partners is first attached covalently to cellulose, and then exposed to the other binding partner. Three common binding partner combinations include protein/ligand, metal ion/chelator, and fusion protein/antibody.…”

Section: Biological Modificationmentioning

confidence: 99%

“…[ 96 ] The second method is specific to cellulose and uses the cellulose‐binding module (CBM), a distinct unit found in cellulase with unique structure, function, and ability to bind to carbohydrates. [ 109 ]…”

Section: Cellulose Biomaterialsmentioning

confidence: 99%

Biofabrication of Cellulose‐based Hydrogels for Advanced Wound Healing: A Special Emphasis on 3D Bioprinting

Tabatabaei Hosseini,

Meadows,

Gabriel

et al. 2023

Macromolecular Bioscience

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Even with the current advancements in wound management, addressing most skin injuries and wounds continues to pose a significant obstacle for the healthcare industry. As a result, researchers are now focusing on creating innovative materials utilizing cellulose and its derivatives. Cellulose, the most abundant biopolymer in nature, has unique properties that make it a promising material for wound healing, such as biocompatibility, tunable physiochemical characteristics, accessibility, and low cost. 3D bioprinting technology has enabled the production of cellulose‐based wound dressings with complex structures that mimic the extracellular matrix. The inclusion of bioactive molecules such as growth factors offers the ability to aid in promoting wound healing, while cellulose creates an ideal environment for controlled release of these biomolecules and moisture retention. The use of 3D bioprinted cellulose‐based wound dressings has potential benefits for managing chronic wounds, burns, and painful wounds by promoting wound healing and reducing the risk of infection. This review provides an up‐to‐date summary of cellulose‐based dressings manufactured by 3D bioprinting techniques by looking into wound healing biology, biofabrication methods, cellulose derivatives, and the existing cellulose bioinks targeted toward wound healing.

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Development of cellulases to improve enzymatic hydrolysis of lignocellulosic biomass

Cited by 6 publications

References 95 publications

Rheological behavior of high consistency enzymatically fibrillated cellulose suspensions

Rheological behavior of high consistency enzymatically fibrillated cellulose suspensions

Systematic bioprospection for cellulolytic actinomycetes in the Chihuahuan Desert: isolation and enzymatic profiling

Biofabrication of Cellulose‐based Hydrogels for Advanced Wound Healing: A Special Emphasis on 3D Bioprinting

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