Development of chitosan/hyaluronic acid hydrogel scaffolds via enzymatic reaction for cartilage tissue engineering

Davachi, Seyed Mohammad; Haramshahi, Seyed Mohammad Amin; Akhavirad, Seyedeh Ava; Bahrami, Naghmeh; Hassanzadeh, Sajad; Ezzatpour, Shahrzad; Hassanzadeh, Nahid; Kebria, Maziar Malekzadeh; Khanmohammadi, Mehdi; Bagher, Zohreh

doi:10.1016/j.mtcomm.2022.103230

Cited by 37 publications

(30 citation statements)

References 68 publications

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“…1 H NMR spectra (Fig. 1B) exhibited new signals around 6.6-7.2 ppm for Ch-Ph attributed to the phenol groups' aromatic ring protons, indicating the successful conjugation of propionic acid into the chitosan backbone [43]. Furthermore, the UV spectra (Fig.…”

Section: Synthesis Of Ch-ph and Alg-ty Conjugatesmentioning

confidence: 93%

An injectable, self-healing, 3D printable, double network co-enzymatically crosslinked hydrogel using marine poly- and oligo-saccharides for wound healing application

Jafari

Alimoradi

Delporte

et al. 2022

Applied Materials Today

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Section: Synthesis Of Ch-ph and Alg-ty Conjugatesmentioning

confidence: 93%

An injectable, self-healing, 3D printable, double network co-enzymatically crosslinked hydrogel using marine poly- and oligo-saccharides for wound healing application

Jafari

Alimoradi

Delporte

et al. 2022

Applied Materials Today

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“…Mitochondrial activity measurement showed a slight reduction of cell viability to approximately 81%, which detected for cardiomyocytes cultivated in each hydrogels containing the Gela‐Ph and even CNT‐Ph substrate until 84 h of cultivation (Figure 3). Meanwhile, this parameter for neat HA‐Ph hydrogel was lowest during cultivation (27% at 84 h) due to poor cell attachment and growth ability 23,27 . Indeed, the Gela is plenteous of cell‐adhesive motifs, leading to desirable cell attachment and proliferation 19,25,32 .…”

Section: Resultsmentioning

confidence: 99%

“…Besides, tri‐compound hydrogel resulted in a water contact angle in 71°–82° which is due to intrinsic hydrophobic behavior of CNT molecules and also Wenzel wetting model that occurs on heterogeneous surface geometries 10,11,44 . The composite hydrogel containing CNT‐Ph could circumvent the poor hydrophilicity of HA‐Ph and provide a desirable microenvironment for cell culture and tissue engineering applications 19,27,45 …”

Section: Resultsmentioning

confidence: 99%

“…The peak at 6.8 ppm is recognized for proton in Ph moiety in the ortho position, and the second peak at 7.2 ppm is known for proton in the meta position. 18,23,27 The Ph moiety contents in HA-Ph, Gela-Ph and CNT-Ph were 1.92 Â 10 À4 (mol Ph/g HA-Ph) and 1.41 Â 10 À4 (mol Ph/g Gela-Ph) and 0.52 (mol Ph/g CNT-Ph), respectively (Figure 2D).…”

Section: Characterization Of Synthesis Biopolymersmentioning

confidence: 99%

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Hyaluronic acid/gelatin microcapsule functionalized with carbon nanotube through laccase‐catalyzed crosslinking for fabrication of cardiac microtissue

Sharifisistani

Khanmohammadi

Badali

et al. 2022

J Biomedical Materials Res

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Carbon nanotube (CNT) and gelatin (Gela) molecules are effective substrates in promoting engineered cardiac tissue functions. This study developed a microfluidic‐based encapsulation process for biomimetic hydrogel microcapsule fabrication. The hydrogel microcapsule was produced through a coaxial double orifice microfluidic technique and a water‐in‐oil emulsion system in two sequential processes. The phenol (Ph) substituted Gela (Gela‐Ph) and CNT (CNT‐Ph), respectively as cell‐adhesive and electrically conductive substrates were incorporated in hyaluronic acid (HA)‐based hydrogel through laccase‐mediated crosslinking. The Cardiomyocyte‐enclosing microcapsule fabricated and cellular survival, function, and possible difference in the biological activity of encapsulated cells within micro vehicles were investigated. The coaxial microfluidic method and Lac‐mediated crosslinking reaction resulted in spherical vehicle production in 183 μm diameter at 500 capsules/min speed. The encapsulation process did not affect cellular viability and harvested cells from microcapsule proliferated well likewise subcultured cells in tissue culture plate. The biophysical properties of the designed hydrogel, including mechanical strength, swelling, biodegradability and electroconductivity upregulated significantly for hydrogels decorated covalently with Gela‐Ph and CNT‐Ph. The tendency of the microcapsule for the spheroid formation of cardiomyocytes inside the proposed microcapsule occurred 3 days after encapsulation. Interestingly, immobilized Gela‐Ph and CNT‐Ph promote cellular growth and specific cardiac markers. Overall, the microfluidic‐based encapsulation technology and synthesized biomimetic substrates with electroconductive properties demonstrate desirable cellular adhesion, proliferation, and cardiac functions for engineering cardiac tissue.

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“…In 2022, Davachi et al fabricated a hyaluronic acid/ chitosan cartilage-like scaffold via horseradish peroxidase enzymatic crosslinking. In vitro experiments showed that mesenchymal stem cells have more likely chondrogenic potential than in control samples, implying that they have promising expression potential for cartilage-like biomarkers [24]. Huang et al constructed a 3D-microenvironment composite comprising GelMA hydrogel with a peptide sequence PFSSTKT-modified chondrocyte extracellular matrix microspheres.…”

Section: Scaffold-dependent Approachesmentioning

confidence: 99%

Recent Biomimetic Approaches for Articular Cartilage Tissue Engineering and Their Clinical Applications: Narrative Review of the Literature

Owida

2022

Advances in Orthopedics

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Since articular cartilage is lacking blood vessels and nerves, its capacity to heal is extremely limited. This means that ruptured cartilage affects the joint as a whole. A health issue known as osteoarthritis can develop as a result of injury and deterioration. Osteoarthritis development can be speeded up by the widespread deterioration of articular cartilage, which ranks third on the list of musculoskeletal disorders requiring rehabilitation, behind only low back pain and broken bones. The current treatments for cartilage repair are ineffective and rarely restore full function or tissue normalcy. A promising new technology in tissue engineering may help create functional cartilage tissue substitutes. Ensuring that the cell source is loaded with bioactive molecules that promote cellular differentiation and/or maturation is the general approach. This review summarizes recent advances in cartilage tissue engineering, and recent clinical trials have been conducted to provide a comprehensive overview of the most recent research developments and clinical applications in the framework of degenerated articular cartilage and osteoarthritis.

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Development of chitosan/hyaluronic acid hydrogel scaffolds via enzymatic reaction for cartilage tissue engineering

Cited by 37 publications

References 68 publications

An injectable, self-healing, 3D printable, double network co-enzymatically crosslinked hydrogel using marine poly- and oligo-saccharides for wound healing application

An injectable, self-healing, 3D printable, double network co-enzymatically crosslinked hydrogel using marine poly- and oligo-saccharides for wound healing application

Hyaluronic acid/gelatin microcapsule functionalized with carbon nanotube through laccase‐catalyzed crosslinking for fabrication of cardiac microtissue

Recent Biomimetic Approaches for Articular Cartilage Tissue Engineering and Their Clinical Applications: Narrative Review of the Literature

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