Development of chromatographic technologies for the quality control of Traditional Chinese Medicine in the Chinese Pharmacopoeia

Shen, Mingrui; He, Yan; Shi, Shang-Mei

doi:10.1016/j.jpha.2020.11.008

Cited by 66 publications

(26 citation statements)

References 20 publications

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“…In addition to the research on active ingredients and their activities, quality control research is also a crucial research work, and it is a necessary monitoring measure to ensure quality. HPLC has the advantages of high separation efficiency, fast analysis speed, high sensitivity, and wide application range, and it is especially suitable for the separation and qualitative and quantitative analysis of complex mixed components [19,20]. In this paper, this technique was used to explore the method for determining the contents of the main active ingredients in mangosteen and the corresponding chromatographic fingerprint, which provided a basis for the quality control and evaluation of mangosteen and was conducive to the further development of this important medicinal plant resource.…”

Section: Discussionmentioning

confidence: 99%

Quantitative Determination and Fingerprint of Phenolic Compounds with Anti-inflammatory Activities for the Quality Control of Mangosteen Extract

Xu¹,

Li²,

Guo³

et al. 2022

Preprint

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The extracts of Garcinia mangostana L. (mangosteen) are traditionally used for medicinal purposes in Asia. Phenols and xanthones are the two main phenolic compounds with anti-inflammatory activities in the pericarps of mangosteen. A simple and economic reverse-phase high-performance liquid chromatography (RP-HPLC) method has been developed for the simultaneous quantification of phenols (catechin, epicatechin, and procyanidin B2) in mangosteen. The mobile phase was acetonitrile: water (12:88) at 30°C, the flow rate was 1 mL/min, and the detection wavelength was 230 nm. The results showed good linear relationships for catechin from 50-250 ng and for epicatechin and procyanidin B2 from 250-1250 ng. The RP-HPLC fingerprint determination method of total xanthones in mangosteen was also established to provide a new method for quality control of mangosteen extract. The mobile phase was a methanol-water gradient elution at 30°C, the flow rate was 1 mL/min, and the detection wavelength was 320 nm. The similarity of fingerprints of 10 batches of total xanthones was more than 0.90. These methods had good precision, reproducibility, and stability and can be applied in the quality control of mangosteen extracts for medicinal purposes.

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Section: Discussionmentioning

confidence: 99%

Quantitative Determination and Fingerprint of Phenolic Compounds with Anti-inflammatory Activities for the Quality Control of Mangosteen Extract

Xu¹,

Li²,

Guo³

et al. 2022

Preprint

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“…Interestingly, none of the samples studied fulfilled this condition, not even when analysed after using ammonia during sample preparation. These results indicate certain shortcomings of the chromatographic methods prescribed by the pharmacopeias for AR samples [ 37 ]. Selection of more reliable detector type is very important for accurate quantification.…”

Section: Discussionmentioning

confidence: 99%

Major bioactive chemical compounds in Astragali Radix samples from different vendors vary greatly

2021

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The worldwide traditional Chinese medicine (TCM) herbs sales figures have increased considerably to 50 billion US$ (2018). Astragali Radix (AR) is amongst the most often sold TCM herbs; sales in the European Union (EU) need European Medicines Agency (EMA) approval. However, comparisons of characteristic bioactive molecules concentrations in AR from different EU vendors are lacking. This study uses liquid chromatography-tandem mass spectrometry (LC-MS/MS) with standard addition to evaluate the influence of different sample and preparation types and ammonia treatment on bioactive molecules concentrations in AR. We also compare AR samples from different EU-vendors. Astragaloside IV (AG-IV), ononin and calycosin 7-O-β-D-glucoside concentrations were higher in root powder samples when extracted with boiled water than with ultrasonication using 70% methanol. AG-IV content was by far the highest in granulates from vendor 1 (202 ± 35 μg/g) but very low in hydrophilic concentrates from vendor 1 (32 ± 7 μg/g) and granulates from vendor 4 (36 ± 3 μg/g). Ammonia-treatment significantly increased AG-IV concentrations in all samples (e.g., to 536 ± 178 μg/g in vendor 1 granulates). Comparable effects were found for most other bioactive molecules. AG-IV and other bioactive molecules concentrations differed strongly depending on sample types, extraction processes, ammonia treatment-or-not and especially between different vendors samples. Ammonia-treatment is debatable, as it is supposed to convert other astragalosides, to AG-IV. The results indicate that routine quantitative analysis of major bioactive compounds present in AR, helps in quality control of AR and to guarantee the quality of commercial products.

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“…19062001) were collected from the province of Guangxi, China. The quality of each crude drugs was strictly ensured according to Chinese Pharmacopoeia to guarantee quality control ( 20 ). HMMCR aqueous extract was prepared according to the following experimental steps: 30 g of HMM and 15 g of CR were soaked in 10 volumes of distilled water for 30 min; then, the mixture was decocted for 1 h, and the resulting supernatant was collected.…”

Section: Methodsmentioning

confidence: 99%

Integrated Network Pharmacology and Experimental Verification to Explore the Molecular Mechanism of Hedysarum Multijugum Maxim–Curcumae Rhizoma Herb Pair for Treating Non-Small Cell Lung Cancer

Zhang

et al. 2022

Front. Oncol.

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BackgroundHedysarum Multijugum Maxim–Curcumae Rhizoma (HMMCR), a well-known herb pair in traditional Chinese medicine (TCM), has been widely used for the treatment of various cancers. However, the active components of HMMCR and the underlying mechanism of HMMCR for non-small-cell lung carcinoma (NSCLC) remain unclear.MethodsActive ingredients of HMMCR were detected by liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). On this basis, potential targets of HMMCR were obtained from SwissTargetPrediction database. NSCLC-related targets were collected from four public databases (GeneCards, OMIM, TTD, and PharmGkb). The drug ingredients–disease targets network was visualized. The hub targets between HMMCR and NSCLC were further analyzed by protein–protein interaction (PPI), Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses. Subsequently, the results predicted by network pharmacology were further validated via in vitro experiments.ResultsA total of 181 compounds were identified from the aqueous extract of HMMCR. Through network analysis, a compound–target network including 153 active ingredients of HMMCR and 756 HMMCR-NSCLC co-targets was conducted; 6 crucial compounds and 62 hub targets were further identified. The results of KEGG enrichment analysis showed that PI3K/Akt signaling pathway may be the critical pathway of HMMCR in the treatment of NSCLC. The in vitro experiments indicated that HMMCR inhibits the proliferation and migration of NSCLC cells via inactivation of the PI3K/Akt signaling pathway, consistent with the results predicted by network pharmacology.ConclusionIntegrating LC-ESI-MS/MS, network pharmacology approach, and in vitro experiments, this study shows that HMMCR has vital therapeutic effect on NSCLC through multi-compound, multi-target, and multi-pathway, which provides a rationale for using HMMCR for the treatment of NSCLC.

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Development of chromatographic technologies for the quality control of Traditional Chinese Medicine in the Chinese Pharmacopoeia

Cited by 66 publications

References 20 publications

Quantitative Determination and Fingerprint of Phenolic Compounds with Anti-inflammatory Activities for the Quality Control of Mangosteen Extract

Quantitative Determination and Fingerprint of Phenolic Compounds with Anti-inflammatory Activities for the Quality Control of Mangosteen Extract

Major bioactive chemical compounds in Astragali Radix samples from different vendors vary greatly

Integrated Network Pharmacology and Experimental Verification to Explore the Molecular Mechanism of Hedysarum Multijugum Maxim–Curcumae Rhizoma Herb Pair for Treating Non-Small Cell Lung Cancer

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