Development of citrate-stabilized Fe3O4 nanoparticles: Conjugation and release of doxorubicin for therapeutic applications

Nigam, Saumya; Barick, K. C.; Bahadur, D.

doi:10.1016/j.jmmm.2010.09.009

Cited by 376 publications

(260 citation statements)

References 45 publications

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“…22 Citric acid, as a stabilizer, not only makes MNPs stable in biological liquids but also provides the uncoordinated carboxyl groups for further functionalization. 23 Thus, citric acid-modified MNPs were fabricated. Under the influence of a magnet, MNPs were not observed to settle down, which confirmed the stability of the colloidal solution.…”

Section: Discussionmentioning

confidence: 99%

Effect of magnetic nanoparticles on apoptosis and cell cycle induced by wogonin in Raji cells

Wang

Zhang²,

Chen³

et al. 2012

IJN

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Traditional Chinese medicine is gradually becoming a new source of anticancer drugs. One such example is wogonin, which is cytotoxic to various cancer cell lines in vitro. However, due to its low water solubility, wogonin is restricted to clinical administration. Recently, the application of drug-coated magnetic nanoparticles (MNPs) to increase water solubility of the drug and to enhance its chemotherapeutic efficiency has attracted much attention. In this study, wogonin was conjugated with the drug delivery system of MNPs by mechanical absorption polymerization to fabricate wogonin-loaded MNPs. It was demonstrated that MNPs could strengthen wogonin-induced cell inhibition, apoptosis, and cell cycle arrest in Raji cells by methylthiazol tetrazolium assay, flow cytometer assay, and nuclear 4′,6-diamidino-2-phenylindole staining. Furthermore, the molecular mechanisms of these phenomena were explored by western blot, in which the protein levels of caspase 8 and caspase 3 were increased significantly while those of survivin and cyclin E were decreased significantly in wogonin-MNPs group. These findings suggest that the combination of wogonin and MNPs provides a promising strategy for lymphoma therapy.

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Section: Discussionmentioning

confidence: 99%

Effect of magnetic nanoparticles on apoptosis and cell cycle induced by wogonin in Raji cells

Wang

Zhang²,

Chen³

et al. 2012

IJN

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“…However, this electrostatic double layer can be easily disassembled by the species in biological media like salts and biomolecules. As for C-SPIONs, the three carboxylate groups of citrate ligand have strong coordination affinity to Fe(III) ions, some of the carboxylate groups strongly coordinate to SPIONs surface, the rest carboxylate groups extend into the water, providing a more robust coating and higher stability to SPIONs in H 2 O and even in some complex media like PBS and RPMI (Nigam et al 2011). …”

Section: Fig 3 Stability Of (A) T-spions and (B) C-spions In H 2 O mentioning

confidence: 99%

Enhanced stability of superparamagnetic iron oxide nanoparticles in biological media using a pH adjusted-BSA adsorption protocol

Laromaine

Roig

2014

J Nanopart Res

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Superparamagnetic iron oxide nanoparticles (SPIONs) are widely used for biological applications due to their unique properties compared to their bulk counterparts, simplified SPIONs stabilization protocols applicable for a wide spectra of biological media remains a challenging issue. In this work, SPIONs with different surface coatings, tetramethylammonium hydroxide coated SPIONs (T-SPIONs) and citrate coated SPIONs (C-SPIONs), were synthesized by a facile, rapid and cost effective microwave assisted method. CSPIONs show robust stability in biological media of PBS and RPMI, while destabilize in DMEM. T-SPIONs were found to aggregate rapidly and significantly in all tested media. Then, a modified pH adjusted-BSA adsorption protocol and an addition of excess trisodium citrate dihydrate (Na 3 Cit) were used to enhance their stability in the media. The BSA adsorption protocol showed great efficiency in stabilizing the dispersed state of both SPIONs in the tested media, while the addition of excess Na 3 Cit showed limit effect, and it was only applicable for C-SPIONs. The formed BSA layer on SPIONs could be imaged by negative staining TEM, and revealed by Cryo-TEM, FTIR, DLS and the zeta potential measurements. Results indicated that BSA forms a monolayer of a thickness of about 3 ± 1 nm and BSA interacts with C-SPIONs and T-SPIONs through the coating of the SPIONs, rather than by replacing them and interacting directly with the SPIONs surface. This synthetic method and stabilization protocol offer a general methodology to obtain SPIONs with a variety of surfactants, stable in different biological media in few minutes.

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“…Citrate ions have been reported to protect the surface of iron oxide nanoparticles from aggregation. [32,33] In order to coat gold shell on the surface of iron oxide nanoparticles, ascorbic acid is used which plays a dual role of being a reducing agent as well as stabilising agent. [34] Here, the reduction of gold (Au 3C ) ions to produce gold shell on to the surface of iron oxide nanoparticles was done by oxidising carbonÀcarbon double bond of ascorbic acid.…”

Section: Resultsmentioning

confidence: 99%

Ascorbic acid-mediated synthesis and characterisation of iron oxide/gold core–shell nanoparticles.

Sood

Arora

Shah

et al. 2015

Journal of Experimental Nanoscience

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(2016) Ascorbic acid-mediated synthesis and characterisation of iron oxide/gold core-shell nanoparticles., Journal of Experimental Nanoscience, 11:5, 370-382, DOI: 10.1080/17458080.2015 The current article reports on providing surface modification of magnetic nanoparticles with gold to provide stability against aggregation. Gold-coated magnetite nanoparticles were synthesised to combine both magnetic as well as surface plasma resonance (SPR) properties in a single moiety. The nanocomposites were produced by reduction (using ascorbic acid) of gold chloride on to the surface of iron oxide nanoparticles. Ascorbic acid not only acts as a reducing agent, but also the oxidised form of ascorbic acid i.e. Dehydroascorbic acid acts as a capping agent to impart stability to as synthesised goldcoated iron oxide nanocomposites. The synthesised nanocomposite was monodispersed with a mean particle size of around 16 nm and polydispersity index of 0.190. X-ray diffraction analysis confirms presence of gold on the surface of magnetite nanoparticles. The synthesised nanocomposites had a total organic content of around 3.2% w/w and also showed a shifted SPR peak at 546 nm as compared to gold nanoparticles (528 nm). Both uncoated and gold-coated magnetite exhibited superparamagnetic behaviour at room temperature. Upon coating with gold shell, saturation magnetisation of iron oxide nanoparticles decreases from 42.806 to 3.54 emu/gram.

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Development of citrate-stabilized Fe3O4 nanoparticles: Conjugation and release of doxorubicin for therapeutic applications

Cited by 376 publications

References 45 publications

Effect of magnetic nanoparticles on apoptosis and cell cycle induced by wogonin in Raji cells

Effect of magnetic nanoparticles on apoptosis and cell cycle induced by wogonin in Raji cells

Enhanced stability of superparamagnetic iron oxide nanoparticles in biological media using a pH adjusted-BSA adsorption protocol

Ascorbic acid-mediated synthesis and characterisation of iron oxide/gold core–shell nanoparticles.

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