Development of Coated Blade Spray Ionization Mass Spectrometry for the Quantitation of Target Analytes Present in Complex Matrices

Gómez‐Ríos, Germán Augusto; Pawliszyn, Janusz

doi:10.1002/anie.201407057

Cited by 223 publications

(187 citation statements)

References 35 publications

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“…By incubating that membrane in a large volume of sample with a subsequent wash step, 10 ng/mL of a small protein could be detected from urine. In another work, a substrate coated with a solid phase microextraction (SPME) material was used to detect low pg/mL levels of small molecule drugs [69]. A large volume of sample was required, however, as well as preconditioning of the SPME substrate, an incubation step in the sample, and a wash step prior to analysis.…”

Section: Other Solid Substratesmentioning

confidence: 99%

Analysis of Biofluids by Paper Spray MS: Advances and Challenges

2016

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Paper spray mass spectrometry (MS) is part of a cohort of ambient ionization or direct analysis methods that seek to analyze complex samples without prior sample preparation. Extraction and electrospray ionization occur directly from the paper substrate upon which a dried matrix spot is stored. Paper spray MS is capable of detecting drugs directly from dried blood, plasma, and urine spots at the low ng/mL to pg/mL levels without sample preparation. No front end separation is performed, so tandem mass spectrometry (MS/MS) or high resolution MS is required. Here, we discuss paper spray methodology, give a comprehensive literature review of the use of paper spray MS for bioanalysis, discuss technological advancements and variations on this technique, and discuss some of its limitations. _________________________________________________________________________________ AbstractPaper spray mass spectrometry (MS) is part of a cohort of ambient ionization or direct analysis methods that seek to analyze complex samples without prior sample preparation. Extraction and electrospray ionization occur directly from the paper substrate upon which a dried matrix spot is stored. Paper spray MS is capable of detecting drugs directly from dried blood, plasma, and urine spots at the low ng/mL to pg/mL levels without sample preparation. No front end separation is performed, so tandem mass spectrometry (MS/MS) or high resolution MS is required. Here, we discuss paper spray methodology, give a comprehensive literature review of the use of paper spray MS for bioanalysis, discuss technological advancements and variations on this technique, and discuss some of its limitations.

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Section: Other Solid Substratesmentioning

confidence: 99%

Analysis of Biofluids by Paper Spray MS: Advances and Challenges

2016

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“…In addition to applications development, methodological advancements have been reported for paper spray MS such as improving selectivity using differential mobility spectrometry prior to MS detection [26], improving sensitivity using solid phase extraction [27], the use of alternative porous substrates [7,[28][29][30], and using paper spray as an ionization source for microfluidics chips [31,32]. Additionally, solid phase microextraction (SPME)-type approaches have been reported in which the SPME material itself served as the spray substrate [33,34].…”

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confidence: 99%

Ionization Suppression and Recovery in Direct Biofluid Analysis Using Paper Spray Mass Spectrometry

Vega

Spence

Zhang

et al. 2016

J. Am. Soc. Mass Spectrom.

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Abstract. Paper spray mass spectrometry is a method for the direct analysis of biofluid samples in which extraction of analytes from dried biofluid spots and electrospray ionization occur from the paper on which the dried sample is stored. We examined matrix effects in the analysis of small molecule drugs from urine, plasma, and whole blood. The general method was to spike stable isotope labeled analogs of each analyte into the spray solvent, while the analyte itself was in the dried biofluid. Intensity of the labeled analog is proportional to ionization efficiency, whereas the ratio of the analyte intensity to the labeled analog in the spray solvent is proportional to recovery. Ion suppression and recovery were found to be compound-and matrix-dependent. Highest levels of ion suppression were obtained for poor ionizers (e.g., analytes lacking basic aliphatic amine groups) in urine and approached -90%. Ion suppression was much lower or even absent for good ionizers (analytes with aliphatic amines) in dried blood spots. Recovery was generally highest in urine and lowest in blood. We also examined the effect of two experimental parameters on ion suppression and recovery: the spray solvent and the sample position (how far away from the paper tip the dried sample was spotted). Finally, the change in ion suppression and analyte elution as a function of time was examined by carrying out a paper spray analysis of dried plasma spots for 5 min by continually replenishing the spray solvent.

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“…For example, Pawliszyn and co-workers used a solid phase microextraction-like con guration for direct coupling of a metal substrate with MS. ey used a stainless steel blade coated with a biocompatible polymer C 18 -polyacrylonitrile as the substrate. 44) is technique, which is termed as coated blade spray, was demonstrated to have the capability to screen 21 compounds that are controlled by World Anti-Doping Agency and the United Nations O ce on Drugs and Crime. 44) …”

Section: Esi Emitter Solid Metal Substratesmentioning

confidence: 99%

“…44) is technique, which is termed as coated blade spray, was demonstrated to have the capability to screen 21 compounds that are controlled by World Anti-Doping Agency and the United Nations O ce on Drugs and Crime. 44) …”

Section: Esi Emitter Solid Metal Substratesmentioning

confidence: 99%

Electrospray Modifications for Advancing Mass Spectrometric Analysis

Meher

Chen

2017

Mass Spectrometry

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Generation of analyte ions in gas phase is a primary requirement for mass spectrometric analysis. One of the ionization techniques that can be used to generate gas phase ions is electrospray ionization (ESI). ESI is a so ionization method that can be used to analyze analytes ranging from small organics to large biomolecules. Numerous ionization techniques derived from ESI have been reported in the past two decades.ese ion sources are aimed to achieve simplicity and ease of operation. Many of these ionization methods allow the exibility for elimination or minimization of sample preparation steps prior to mass spectrometric analysis. Such ion sources have opened up new possibilities for taking scienti c challenges, which might be limited by the conventional ESI technique. us, the number of ESI variants continues to increase. is review provides an overview of ionization techniques based on the use of electrospray reported in recent years. Also, a brief discussion on the instrumentation, underlying processes, and selected applications is also presented.

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Development of Coated Blade Spray Ionization Mass Spectrometry for the Quantitation of Target Analytes Present in Complex Matrices

Cited by 223 publications

References 35 publications

Analysis of Biofluids by Paper Spray MS: Advances and Challenges

Analysis of Biofluids by Paper Spray MS: Advances and Challenges

Ionization Suppression and Recovery in Direct Biofluid Analysis Using Paper Spray Mass Spectrometry

Electrospray Modifications for Advancing Mass Spectrometric Analysis

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