Development of Colonic Organoids Containing Enteric Nerves or Blood Vessels from Human Embryonic Stem Cells

Park, Chul Soon; Nguyen, Le Phuong Anh; Yong, Dongeun

doi:10.3390/cells9102209

Cited by 21 publications

(15 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…62 Current models have also utilised the PSC system that can be differentiated towards intestinal organoids, as well as cells of the ENS such as neural crest cells (NCCs). [63][64][65] NCCs that were cocultured with developing intestinal organoids migrated into the mesenchyme, differentiated into neurons and glial cells and showed neuronal activity. 63 ENS-containing intestinal organoids formed neuroglial structures similar to a myenteric and submucosal plexus in vivo.…”

Section: Innervated Organoids (Epithelial-neural Interaction)mentioning

confidence: 99%

“… 73 Indeed, several attempts have been made to introduce iPSC-derived endothelial cells into GI organoids. 65 74 75 Interestingly, a recent study uncovered a population of endothelial cells which are present early in iPSC differentiation towards intestinal organoids that declines over time in culture. This study further developed a method to expand and maintain this endothelial cell popoulation within intestinal organoids as an alternative approach for classical vascularised organoid models.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Organoids in gastrointestinal diseases: from experimental models to clinical translation

Günther

Winner

Stappenbeck

2022

Gut

View full text Add to dashboard Cite

We are entering an era of medicine where increasingly sophisticated data will be obtained from patients to determine proper diagnosis, predict outcomes and direct therapies. We predict that the most valuable data will be produced by systems that are highly dynamic in both time and space. Three-dimensional (3D) organoids are poised to be such a highly valuable system for a variety of gastrointestinal (GI) diseases. In the lab, organoids have emerged as powerful systems to model molecular and cellular processes orchestrating natural and pathophysiological human tissue formation in remarkable detail. Preclinical studies have impressively demonstrated that these organs-in-a-dish can be used to model immunological, neoplastic, metabolic or infectious GI disorders by taking advantage of patient-derived material. Technological breakthroughs now allow to study cellular communication and molecular mechanisms of interorgan cross-talk in health and disease including communication along for example, the gut–brain axis or gut–liver axis. Despite considerable success in culturing classical 3D organoids from various parts of the GI tract, some challenges remain to develop these systems to best help patients. Novel platforms such as organ-on-a-chip, engineered biomimetic systems including engineered organoids, micromanufacturing, bioprinting and enhanced rigour and reproducibility will open improved avenues for tissue engineering, as well as regenerative and personalised medicine. This review will highlight some of the established methods and also some exciting novel perspectives on organoids in the fields of gastroenterology. At present, this field is poised to move forward and impact many currently intractable GI diseases in the form of novel diagnostics and therapeutics.

show abstract

Section: Innervated Organoids (Epithelial-neural Interaction)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Organoids in gastrointestinal diseases: from experimental models to clinical translation

Günther

Winner

Stappenbeck

2022

Gut

View full text Add to dashboard Cite

show abstract

“…The development of colonic organoids from PSCs has recently advanced. Park et al [35] reported a method that generated colonic organoids containing enteric nerves or blood vessels by co-culturing nerve cells or endothelial cells with colonic organoids. The NCCs and endothelial cells used in that study were all differentiated from the same hPSC line as the colonic organoids, and integration into organoids was performed after the hindgut was defined from the definitive endoderm.…”

Section: ) Colon (Large Intestine)mentioning

confidence: 99%

Technical advances in pluripotent stem cell-derived and tumorigenic organoids

et al. 2022

View full text Add to dashboard Cite

Cell culture systems have been widely used to address fundamental questions in biology without sacrificing animals. Three-dimensional (3D) organoids provide more information on in vivo conditions than traditional culture systems because multiple cell types in organoids interact with each other in 3D structures. Despite extensive research and advances in the organoid field, some important limitations remain and need further consideration. In this review, we summarize how organoids are generated from pluripotent stem cells and describe the recent technical progress that has made organoids more similar to in vivo tissues for the application of organoids to modeling cancer. Lastly, we briefly discuss some limitations that have been raised in this field.

show abstract

“…When combined, vagal NC progenitors generated glia and neurons of the ENS which integrated into the smooth muscle layer of the HIO and were found to promote cell proliferation in intestinal crypts and gene expression indicative of intestinal cell development. More recent work has described the generation of assembloid cultures containing colonic intestinal tissue with ENS and blood vessels; a model perhaps more suited to diseases which impact the distal part of the GI tract, including Crohn's and Hirschsprung's disease [ 88 ].…”

Section: Development In Vitro : Generating Nc Cell...mentioning

confidence: 99%

Shaping axial identity during human pluripotent stem cell differentiation to neural crest cells

Cooper

Tsakiridis

2021

Biochemical Society Transactions

View full text Add to dashboard Cite

The neural crest (NC) is a multipotent cell population which can give rise to a vast array of derivatives including neurons and glia of the peripheral nervous system, cartilage, cardiac smooth muscle, melanocytes and sympathoadrenal cells. An attractive strategy to model human NC development and associated birth defects as well as produce clinically relevant cell populations for regenerative medicine applications involves the in vitro generation of NC from human pluripotent stem cells (hPSCs). However, in vivo, the potential of NC cells to generate distinct cell types is determined by their position along the anteroposterior (A–P) axis and, therefore the axial identity of hPSC-derived NC cells is an important aspect to consider. Recent advances in understanding the developmental origins of NC and the signalling pathways involved in its specification have aided the in vitro generation of human NC cells which are representative of various A–P positions. Here, we explore recent advances in methodologies of in vitro NC specification and axis patterning using hPSCs.

show abstract

Development of Colonic Organoids Containing Enteric Nerves or Blood Vessels from Human Embryonic Stem Cells

Cited by 21 publications

References 37 publications

Organoids in gastrointestinal diseases: from experimental models to clinical translation

Organoids in gastrointestinal diseases: from experimental models to clinical translation

Technical advances in pluripotent stem cell-derived and tumorigenic organoids

Shaping axial identity during human pluripotent stem cell differentiation to neural crest cells

Contact Info

Product

Resources

About