Development of criteria for a comprehensive assessment of the effectiveness of antiangiogenic drugs on models of neovascularization of the eye (experimental studies)

Abstract

The evolution of therapeutic technologies dictates the development of a strategy for their implementation in clinical practice. The first stage is the assessment of the capabilities, efficiency, advantages and disadvantages, development of indications and contraindications on models of eye diseases in the experiment.The purpose of the work: to develop criteria for assessing the effectiveness of anti‑angiogenic drugs and evaluate the proposed modeland neovascularization of the eye in the experiment.Materials and methods. Neoangiogenesis models – two chorioretinal and two corneal localizations – were formed in the experiment on rabbits of Sovetskaya Chinchilla breed. The advantages and disadvantages of each of them were analyzed empirically. Experimental animals with neovascular disease were treated with recombinant peptides with angiostatic potential (tumstatin, PEDF, endostatin). In the course of treatment, we developed efficacy criteria based on clinical, instrumental, and morphological studies.Results. Comprehensive efficacy evaluation criteria were developed and tested, allowing not only to reveal and describe the spectrum of biological effects of the tested drugs, but also to quantitatively assess and compare their therapeutic potential with each other at almost all stages of vascular assembly. The complex consisted of qualitative and quantitative clinical (hyperemia, edema, vascularization, antiangiogenic activity according to a score scale), angiographic (intensity and area of edema, area of neovascularization and index of corneal vascularization) and morphological (extent of edema, extent of vessels and their number in section, index of vascularization) indicators.Conclusion. To get the full amount of information about the tested drug with the claimed angiostatic potential, it is necessary to use several models of neoangiogenesis with different mechanisms to study the amplitude and spectrum of their effects.