Development of cyanobacterium-based biofilms and their in vitro evaluation for agriculturally useful traits

Prasanna, Radha; Pattnaik, Sasmita; Sugitha, T.C.K.; Nain, Lata; Saxena, Anil Kumar

doi:10.1007/s12223-011-0013-5

Cited by 80 publications

(36 citation statements)

References 28 publications

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“…subtilis-and Trichoderma-based biofilms such as Trichoderma-Azotobacter and Trichoderma-Pseudomonas were prepared as outlined by Prasanna et al (2011) andTriveni et al (2015). subtilis-and Trichoderma-based biofilms such as Trichoderma-Azotobacter and Trichoderma-Pseudomonas were prepared as outlined by Prasanna et al (2011) andTriveni et al (2015).…”

Section: Growth and Maintenance Of Organisms Used In This Studymentioning

confidence: 99%

Microbial Inoculants with Multifaceted Traits Suppress Rhizoctonia Populations and Promote Plant Growth in Cotton

Prasanna

Ramakrishnan

Ranjan

et al. 2016

Journal of Phytopathology

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The suppressive effects of microbial inoculants on cotton seedling mortality were assessed in Rhizoctonia solani‐infested soil. Per cent mortality ranged from 16 to 32 (60–120 days after sowing, DAS) and significant differences were recorded at 120 DAS, especially after drenching with compost tea of Azotobacter sp. and Anabaena torulosa—Trichoderma viride‐biofilmed formulations. The activity of hydrolytic enzymes was reduced in diseased root tissues due to a majority of the microbially inoculated treatments, compared with healthy root tissues. Per cent changes in the amounts of glomalin‐related soil proteins (GRSPs) were 2 to 85% greater than those of the uninoculated experimental controls. These microbial inoculants altered the rhizosphere bacterial communities as evident from the Denaturing gradient gel electrophoresis (DGGE) banding patterns and, also reduced the population of R. solani. While the copy numbers of the internal transcribed spacer (ITS) gene of R. solani in the uninoculated (infested soil) were approximately 1.47 × 1011 per g soil, they were 1.34–1.42 × 105 per g soil after the application of A. torulosa, Anabaena laxa and A. torulosa–Bacillus sp. Increases in yield (ranging from 3 to 23%) due to various microbial inoculants relative to uninoculated controls illustrated their promise as plant growth‐promoting and disease‐suppressing agents. This study illustrates the modulation of rhizosphere ecology through microbial inoculants as a mechanism of disease suppression and sustaining plant growth.

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Section: Growth and Maintenance Of Organisms Used In This Studymentioning

confidence: 99%

Microbial Inoculants with Multifaceted Traits Suppress Rhizoctonia Populations and Promote Plant Growth in Cotton

Prasanna

Ramakrishnan

Ranjan

et al. 2016

Journal of Phytopathology

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“…Samples were removed periodically and examined under a microscope to monitor the colonization of cyanobacterial filaments by bacterial -fungal cultures. Growth was measured spectrophotometrically in terms of chlorophyll, using methanolic extracts of the biofilms (Mackinney 1941;Prasanna et al 2011) after 4, 6 and 9 weeks.…”

Section: Experimental Set-up For Biofilm Preparationmentioning

confidence: 99%

Deciphering the biochemical spectrum of novel cyanobacterium-based biofilms for use as inoculants

Prasanna

Kumar

Babu

et al. 2013

Biological Agriculture & Horticulture

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An investigation was done using cyanobacterium Anabaena torulosa as a matrix for developing novel biofilms, with anti-grazer traits against microfauna/pathogenic fungi, through co-inoculation of agriculturally important bacteria and fungi. The biofilms generated were evaluated after 4, 6 and 9 weeks of incubation for the activity of hydrolytic enzymes and fungicidal activity against phytopathogenic fungi. The activity of b-1,3-glucanase, in general, showed a gradual increasing trend up to 9 weeks, while endoglucanase activity was highest after 6 weeks of incubation, and a 40 -50% reduction in chitosanase activity was recorded by the end of 9 weeks of incubation. Observations revealed that the fungus -cyanobacterium biofilms, especially Anabaena-Aspergillus awamori, exhibited the highest activity of b-1,3-glucanase and ranked second in terms of chitosanase activity. Fungicidal activity was recorded up to 9 weeks in most of the biofilms, and the highest values were recorded in cyanobacterium -Bacillus and cyanobacterium -fungus biofilms. Such biofilms were also tested against selected nematodes in microcosm experiments, which revealed no significant deleterious effects. The biocontrol activity of such biofilmed preparations against phytopathogenic fungi, but not towards selected nematodes, illustrates their promise in agriculture as potential inoculants that can effectively establish in soil.

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“…The use of microbial inoculation in the nutrient management of rice is known to elicit positive effects on growth, yield, and nutrient uptake. Among microbes, the role of cyanobacteria and their consortium/biofilms with agriculturally beneficial bacteria in improving plant growth by N fixation and solubilizing soil P has been reported, and its promise is well established in conventional puddled transplanted rice system, besides recent reports on their promise in SRI, but not in aerobic rice . However, no information is available regarding the comparative efficacy of these inoculants in SRI and ARS, vis‐à‐vis PTR, in terms of improving yield and water savings; with this background, this study was undertaken.…”

Section: Introductionmentioning

confidence: 99%

Improving Water and Nutrient Use Efficiency in Rice by Changing Crop Establishment Methods, Application of Microbial Inoculations, and Zn Fertilization

et al. 2019

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A field experiment was conducted during the wet seasons of 2013 and 2014 to evaluate the effect of three rice establishment methods: viz. puddled transplanted rice (PTR), system of rice intensification (SRI) and aerobic rice system (ARS), two cyanobacteria based inoculants, viz. Anabaena sp (CR1) + Providencia sp (PR3) consortium and Anabaena–Pseudomonas (An‐Ps) biofilm formulation, and zinc (Zn) fertilization on the rice yield, water productivity, and nutrient use efficiency. The yields of rough, brown and milled rice were highest in SRI, which was on par with PTR and both methods proved significantly superior to ARS in both years. The total water productivity of rough and brown rice in the first year was significantly higher in SRI. The SRI method saved 21.9% and 27.4% irrigation water over PTR, and savings in ARS were 37.4% and 50.8% in first and second year respectively, over PTR. The use of An‐Ps biofilm formulation along with 75% RDN improved the agronomic use efficiency of both nitrogenous and phosphatic fertilizers applied. On the basis of the present study, it can be concluded that SRI improved rice yields and water productivity; while involvement of An‐Ps biofilm formulation can be recommended for improved nutrient use efficiency.

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Development of cyanobacterium-based biofilms and their in vitro evaluation for agriculturally useful traits

Cited by 80 publications

References 28 publications

Microbial Inoculants with Multifaceted Traits Suppress Rhizoctonia Populations and Promote Plant Growth in Cotton

Microbial Inoculants with Multifaceted Traits Suppress Rhizoctonia Populations and Promote Plant Growth in Cotton

Deciphering the biochemical spectrum of novel cyanobacterium-based biofilms for use as inoculants

Improving Water and Nutrient Use Efficiency in Rice by Changing Crop Establishment Methods, Application of Microbial Inoculations, and Zn Fertilization

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