Development of Detection Method of Unapproved Genetically Modified Potato (EH92-527-1) in Korea using Duplex Polymerase Chain Reaction

유명렬,; 김재환,; 예미지,; 김해영,

doi:10.9721/kjfst.2013.45.2.156

Cited by 5 publications

(3 citation statements)

References 11 publications

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“…또한, 제초제저항성 LM 옥수수 DAS-40278-9 (aad-1 유전자 삽입)와 LM 캐놀라 MON88302 (cp4 epsps 유전자 삽 입) PCR 반응 결과, 유전자변형 옥수수와 캐놀라에 대해서 만 특이적으로 도입유전자가 검출되는 것을 확인하였다 ( Fig. 3E and F Kim et al 2008;Kim et al 2015;Kim et al 2009b;Lee et al 2007;Min et al 2004;Moon et al 2012;Wu et al 2009;Yoo et al 2013 …”

Section: A-d)unclassified

Development of detection methods for six approved LM crops in Korea

Seol

Choi

et al. 2017

J Plant Biotechnol

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Abstract Living modified crops are genetically modified living organisms and are widely used in biotechnical research and desired goods. As the reliance on LM products, concerns about safety of LMOs have been continuously increased in South Korea. We established the detection methods for unintentional released LMOs in environmental conditions. To detect six LM event genes of 1 canola, 1 maize and 4 soybeans, PCR conditions were based upon consideration of the Joint Research Centre information. Genomic DNAs were isolated from LM samples and PCR analysis were performed using each event-specific primer pair. Event-specific genes of all events were efficiently recognized by our methods. To investigate the insertion site of LM genes in each genome, we verified PCR product sequence by DNA sequencing. These results suggest that the LM event-specific gene amplification can be efficiently developed. In addition, our detection method is fit for monitoring and post-management of LM crops in the environment.

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Section: A-d)unclassified

Development of detection methods for six approved LM crops in Korea

Seol

Choi

et al. 2017

J Plant Biotechnol

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“…Several studies have been conducted to develop methods to identify GM potatoes. Detection methods for NewLeaf, NewLeaf Y, NewLeaf Plus, PH05-026-0048, EH92-527-1, AM04-1020, SPS-E12, AV43-6-G7, and insertion Cry3A have been developed using conventional PCR (PCR), real-time PCR (qPCR) (Cote et al, 2005 ; Roh et al, 2004 ; Song et al, 2017 ; Yoo et al, 2013 ), loop-mediated isothermal amplification (Tu et al, 2020 ), and droplet digital PCR (Gao et al, 2016 ). Recent gene detection assays are directed towards simpler and more cost-effective methods.…”

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confidence: 99%

Detection method for genetically modified potato using an ultra-fast PCR system

Shin

Jeon

Koo

2023

Food Sci Biotechnol

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Genetically modified (GM) potatoes having resistance to insects and viral diseases, low reducing sugar contents, and black spots for high quality continue to be developed. However, no GM potato has been approved as food or feed in the Republic of Korea as the country adheres to a zero-tolerance policy to unauthorized genetically modified organisms (GMOs). When the self-sufficiency rate is low, a detection method to assess GMOs in crops or other products is necessary. Therefore, a rapid method for two GM potato events (SPS-Y9 and EH92-527-1) using an ultra-fast PCR (UF-PCR) system has been developed, and its specificity, sensitivity, and applicability were demonstrated. UF-PCR can decrease the runtime of PCR by more than half of that needed in conventional methods. However, UF-PCR is not a common method for GMO analysis. This rapid detection method may be useful for GMO analyses in field conditions.

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“…1). 하지만 이 와 같은 multiplex PCR 검출법에 대한 연구는 LM 콩, 감 자, 옥수수와 같이 두부, 전분 생산을 위한 식품용 GMO 와 사료원료 작물에 국한되어 개발된 사례가 보고된바 있 다(Kim et al 2013;Yoo et al 2013).LM 면화 이벤트에 대해서는 단일이벤트 특이적인 정량 적 및 정성적 분석법이 개발된 바 있고(Yang et al 2005;Baeumler et al 2015), 면화의 경우 4개(MON1445, MON15985, MON88319, LLCOTTON25) 면화 이벤트에 대한 동시 검 출용 마커가 개발된 사례가 있다(Kim et al 2008). 하지만 DNA 상태로 구입된 표준물질과 함께 PCR의 주형으로 사용하였다.Genomic DNA 순수 정제 PCR 주형으로 사용하기 위하여 분말상태로 구입된 LM 면화 이벤트 표준물질에서 genomic DNA를 정제하였다.…”

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Multiplex PCR method for environmental monitoring of approved LM cotton events in Korea

Seol

Shin

et al. 2016

J Plant Biotechnol

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The growth area of living modified (LM) cotton has steadily increased every year, since its first commercialization in 1996. Development of environmental risk assessment tools and techniques for LM cotton is required for ecosystem safety. We therefore developed multiplex PCR assays for simultaneous detection of two (MON15985, MON531) and four (GHB614, LLCOTTON25, MON88913 and MON1445) LM cotton events approved in Korea, with event specific primer pairs. The PCR reactions were optimized by using event specific primers of six LM cottons at various concentrations. The reactions allows amplification of estimated amplicons of MON15985 (214 bp), MON531 (270 bp), GHB614 (119 bp), LLCOTTON25 (164 bp), MON88913 (276 bp), and MON1445 (389 bp) from multiplex PCR reactions. The multiplex PCR assay developed allowed that two annealing steps (15 cycles at 55°C and 25 cycles at 60°C) were performed for amplification of distinguished two LM cottons, and only one annealing step (50 cycles at 60°C) was necessary for tetraplex PCR. Primer extension step of all PCR reactions was skipped for time-effective amplification. Our methods suggest that two multiplex PCR assays can be cost-effective and a rapid diagnostic tool for environmental LMO monitoring of six LM cottons.

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Development of Detection Method of Unapproved Genetically Modified Potato (EH92-527-1) in Korea using Duplex Polymerase Chain Reaction

Cited by 5 publications

References 11 publications

Development of detection methods for six approved LM crops in Korea

Development of detection methods for six approved LM crops in Korea

Detection method for genetically modified potato using an ultra-fast PCR system

Multiplex PCR method for environmental monitoring of approved LM cotton events in Korea

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