Development of diagnostic molecular markers for marker-assisted breeding against bacterial wilt in tomato

Abebe, Alebel Mekuriaw; Choi, Ju Young; Kim, Young‐Jun; Oh, Chang‐Sik; Yeam, Inhwa; Nou, Ill Sup; Lee, Je Min

doi:10.1270/jsbbs.20027

Cited by 21 publications

(11 citation statements)

References 46 publications

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“…From this study, genotypes LA 0442, LA 2443, LA 0376 and H7996 recorded the lowest levels of incidence and severity of the BW disease throughout the study. The variation in the level of BW incidence and severity among the tomato genotypes observed in this study could be due to the differences in their genetic makeup as reported by [30] who screened 27 cultivars for resistance to bacterial wilt disease and identified resistant genotypes with low mean disease severity scores in several genotypes including Hawaii 7996, BWR-20, Spider, High Power, IT 201664, Hawaii 7998, and Fighting. Comparing the resistance level using the susceptible check Petofake as a reference, H7996, LA 0442 and LA 0443 were of higher resistance to BW disease followed by LA 0376, AVTO 1717 and LA 2701.…”

Section: Discussionmentioning

confidence: 51%

Screening Tomato Genotypes for Bacterial Wilt Disease (Ralstonia solanacearum) Resistance in Ghana

B-Mensah¹,

Osei²,

Prempeh³

2021

EJFOOD

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Thirteen-tomato genotypes were assembled from the USA, Taiwan and Ghana for screening for bacterial wilt resistance in two environments: in a screen house at Crops Research Institute, Kumasi, Ghana and in the field at a bacterial wilt hot spot at Bechem in the Brong-Ahafo region of Ghana. The bacterium, Ralstonia solanacearum was isolated using the serial dilution method on Nutrient Agar (NA) and aseptically cultured for inoculation. The bacterium was implicated as the causal organism of the bacterial wilt disease of tomato. A local check, Petofake and H7996 were used as susceptible and resistant checks respectively. Three genotypes, H7996, LA0442 and LA0443 demonstrated significant resistance stability in both experimental environments by recording significantly low wilt incidence and severity. H7996 was obtained from the World Vegetable Centre (WVC), Taiwan while the other two genotypes were collected from the University of California, Davis, USA. Apart from the highest yielding genotype LA 0442 that recorded 50.67 t/h, the rest recorded very low yields. The lowest yielding genotype AVTO 1713, recorded (12.67 t/h). Thus, the highest yielding genotype, LA 0442 over-yielded the lowest AVTO1713 by approximately 300%. It is instructive to note that LA0442, which was identified as a stable resistant genotype also recorded the highest yield. This result supports the potential of developing a resistant, high yielding variety using LA0442 as a parent. The future of the tomato industry in Ghana therefore looks promising.

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Section: Discussionmentioning

confidence: 51%

Screening Tomato Genotypes for Bacterial Wilt Disease (Ralstonia solanacearum) Resistance in Ghana

B-Mensah¹,

Osei²,

Prempeh³

2021

EJFOOD

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“…Forty-seven tomato cultivars were evaluated for bacterial canker resistance including previously reported cultivars such as resistant “IRAT L-3” ( Stüwe and von Tiedemann, 2013 ) and susceptible “Moneymaker” ( Mohd Nadzir et al, 2019 ). For the qualitative resistance screening, cultivars were classified as resistant (disease severity score ≤ 2.0) and susceptible (disease severity score ≥ 3.0; Bartkiewicz et al, 2018 ; Abebe et al, 2020 ). Accordingly, 11 cultivars were grouped as resistant and 36 as susceptible ( Table 1 ).…”

Section: Resultsmentioning

confidence: 99%

QTL-Seq Analysis for Identification of Resistance Loci to Bacterial Canker in Tomato

Abebe

Kim

et al. 2022

Front. Plant Sci.

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Bacterial canker caused by Clavibacter michiganensis (Cm) is one of the most economically important vascular diseases causing unilateral leaf wilting, stem canker, a bird’s-eye lesion on fruit, and whole plant wilting in tomato. There is no commercially available cultivar with bacterial canker resistance, and genomics-assisted breeding can accelerate the development of cultivars with enhanced resistance. Solanum lycopersicum “Hawaii 7998” was found to show bacterial canker resistance. A Quantitative trait loci (QTL)-seq was performed to identify the resistance loci using 909 F2 individuals derived from a cross between S. lycopersicum “E6203” (susceptible) and “Hawaii 7998,” and a genomic region (37.24–41.15 Mb) associated with bacterial canker resistance on chromosome 6 (Rcm6) was found. To dissect the Rcm6 region, 12 markers were developed and several markers were associated with the resistance phenotypes. Among the markers, the Rcm6-9 genotype completely matched with the phenotype in the 47 cultivars. To further validate the Rcm6 as a resistance locus and the Rcm6-9 efficiency, subsequent analysis using F2 and F3 progenies was conducted. The progeny individuals with homozygous resistance allele at the Rcm6-9 showed significantly lower disease severity than those possessing homozygous susceptibility alleles. Genomes of five susceptible and two resistant cultivars were analyzed and previously known R-genes were selected to find candidate genes for Rcm6. Nucleotide-binding leucine-rich repeat, receptor-like kinase, and receptor-like protein were identified to have putative functional mutations and show differential expression upon the Cm infection. The DNA markers and candidate genes will facilitate marker-assisted breeding and provide genetic insight of bacterial canker resistance in tomato.

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“…Diagnostic markers are molecular markers that are significantly linked with one or more traits, which help in the early and rapid identification of a target phenotype ( Chakdar et al., 2019 ). Diagnostic markers have been successfully employed in many traits of various plants, such as the rust resistance trait in wheat ( Liu et al., 2021 ) and common wheat ( Wu et al., 2018 ), the testa color in cultivated peanut ( Chen et al., 2021 ), and the bacterial wilt trait in tomato ( Abebe et al., 2020 ). In the present study, three Indel markers located in the PH1.1 region were validated to be closely linked with the plant height of broomcorn millet using a total of 512 accessions.…”

Section: Discussionmentioning

confidence: 99%

Identification and fine-mapping of a major QTL (PH1.1) conferring plant height in broomcorn millet (Panicum miliaceum)

Liu

et al. 2022

Front. Plant Sci.

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The plant height of broomcorn millet (Panicum miliaceum) is a significant agronomic trait that is closely related to its plant architecture, lodging resistance, and final yield. However, the genes underlying the regulation of plant height in broomcorn millet are rarely reported. Here, an F2 population derived from a cross between a normal variety, “Longmi12,” and a dwarf mutant, “Zhang778,” was constructed. Genetic analysis for the F2 and F2:3 populations revealed that the plant height was controlled by more than one locus. A major quantitative trait locus (QTL), PH1.1, was preliminarily identified in chromosome 1 using bulked segregant analysis sequencing (BSA-seq). PH1.1 was fine-mapped to a 109-kb genomic region with 15 genes using a high-density map. Among them, longmi011482 and longmi011489, containing nonsynonymous variations in their coding regions, and longmi011496, covering multiple insertion/deletion sequences in the promoter regions, may be possible candidate genes for PH1.1. Three diagnostic markers closely linked to PH1.1 were developed to validate the PH1.1 region in broomcorn millet germplasm. These findings laid the foundation for further understanding of the molecular mechanism of plant height regulation in broomcorn millet and are also beneficial to the breeding program for developing new varieties with optimal height.

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Development of diagnostic molecular markers for marker-assisted breeding against bacterial wilt in tomato

Cited by 21 publications

References 46 publications

Screening Tomato Genotypes for Bacterial Wilt Disease (Ralstonia solanacearum) Resistance in Ghana

Screening Tomato Genotypes for Bacterial Wilt Disease (Ralstonia solanacearum) Resistance in Ghana

QTL-Seq Analysis for Identification of Resistance Loci to Bacterial Canker in Tomato

Identification and fine-mapping of a major QTL (PH1.1) conferring plant height in broomcorn millet (Panicum miliaceum)

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