Development of disposable low density screen-printed electrode arrays for simultaneous electrochemical measurements of the hybridisation reaction

Laschi, Serena; Palchetti, Ilaria; Marrazza, Giovanna; Mascini, Marco

doi:10.1016/j.jelechem.2006.04.015

Cited by 57 publications

(27 citation statements)

References 19 publications

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“…Laschi et al [9] have reported on the use of carbon and gold low density SPEs assays for simultaneous electrochemical measurement of hybridization reactions, Lucarelli et al [10] described the analytical performance of an enzyme-based electrochemical genosensor based on oligonucleotidemodified gold SPEs using impedimetric detection and, more recently, Farabullini et al [11] have described a screen-printed array of four gold electrodes for the analysis of bacterial food contaminants at the nanomolar level, based on the immobilization of thiol labeled DNA probes and the use of biotinylated signaling probes coupled with a streptavidin-alkaline phosphatase conjugate and then exposed to an a-naphthyl phosphate solution.…”

Section: Introductionmentioning

confidence: 99%

Designs of Enterobacteriaceae Lac Z Gene DNA Gold Screen Printed Biosensors

et al. 2008

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This paper describes specific electrochemical enterobacteriaceae lac Z gene DNA sensors based on immobilization of a thiolated 25 base single stranded probe onto disposable screen printed gold electrodes (gold SPEs). Two configurations have been evaluated. In the first one, the capture probe was attached to the electrode surface through its ÀSH moiety, while mercaptohexanol (MCH) was used as spacer for the displacement of nonspecifically adsorbed oligonucleotide molecules. The hybridization event between the probe and target DNA sequences was detected at À 0.20 V by square-wave voltammetry (SWV), using methylene blue (MB) as electrochemical indicator. The second genosensor configuration involved modification of gold high temperature SPEs with a 3,3'-dithiodipropionic acid di(N-succinimidyl ester) (DTSP) self-assembled monolayer (SAM). Moreover, 2-aminoethanol was used as blocking agent, and further modification with avidin allowed binding of the biotinylated enterobacteriaceae lac Z gene DNA probe. An enzyme amplified detection scheme was applied, based on the coupling of streptavidin-peroxidase to the biotinylated complementary target, after the hybridization process, and immobilization of tetrathiafulvalene (TTF) as redox mediator atop the modified electrode. The amperometric response obtained at À 0.15 V after the addition of hydrogen peroxide was used to detect the hybridization process. Experimental variables concerning sensors composition and electrochemical transduction were evaluated in both cases. A better precision and reproducibility in the fabrication process, as well as a higher sensitivity were achieved using the biotinylated probe-based sensor configuration. A limit of detection of 0.002 ng/mL was obtained without any preconcentration step.

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Section: Introductionmentioning

confidence: 99%

Designs of Enterobacteriaceae Lac Z Gene DNA Gold Screen Printed Biosensors

et al. 2008

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“…The selectivity of the Nafion/AgHCF/GSPE was studied by detecting the response of the modified electrode for 1 mM AA in presence of whereas in the presence of AA is 71 mV, which indicates that, in terms of reversibility, the redox response attributed to the Fe(III)/(II) process is not affected much by the presence of AA. The deviation from the theoretical value of 59 mV for an ideal reversible process can be due to the polymeric binder present in the film which can affect the conductivity of the film 38 . It is possible that the binder could cover some of the active sites of the electrode surface as well.…”

Section: Interference Studiesmentioning

confidence: 89%

Screen-Printed Electrode Modified With Silver Hexacy Anoferrate-Nafion® for Ascorbic Acid Determination

Mattos

Padilla

Zagal

et al. 2011

J. Chil. Chem. Soc.

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A flow injection system using a new and/or re-used graphite screen-printed electrode modified with silver hexacyanoferrate and a Nafion ® polymer layer (AgHCF/GSPE) was employed for the determination of ascorbic acid in orange juice and drugs samples. Both modified electrodes showed an initial sensitivity of 0.015 A.cm . The response of the electrodes was stable, with no variation of base line after 6-8 hours of continuous operation. With this system it is possible to measure 65-70 samples (20µL) per hour. By using 1.06 mg of AgNO 3 , 4.12 mg of K 3 Fe(CN) 6 , 50.5 mg of KNO 3 and 800µL of HNO 3 , it is possible to obtain between 25 and 30 modified electrodes. The proposed system showed to be inexpensive, versatile, robust and suitable for industrial application. The surface morphology of the bare and/or modified graphite screen-printed electrode was characterized by scanning electron microscopy.

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“…This result differs from those observed in previous works on screen- printed Au and Pt electrodes, in which the difference between anodic and cathodic peak potentials is greater than the expected for a reversible couple. The deviations from theoretical values are attributed to the binder in the ink composition covering some active sites of the electrodes [10,15]. To explain this behavior a model was developed by Amatore et al based on nonlinear diffusion effects due partial coverage [16].…”

Section: Electrochemical Characterization and Applicationsmentioning

confidence: 99%

“…One of the limitations when tracks and electrodes are made with graphite ink is its relative low conductivity, which in the case of cyclic voltammetry produces the equivalent effect of an ohmic drop, limiting their use. On the other hand, gold screen printed electrodes, even though they present a good conductivity are expensive and present a behavior that corresponds to an electrode surface partially covered [10].…”

Section: Introductionmentioning

confidence: 99%

Disposable Gold Electrode Array for Simultaneous Electrochemical Studies

et al. 2007

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An efficient and inexpensive eight gold electrode array has been manufactured by a combination of screen printing and gold electrodeposition techniques. Gold electrodeposition was performed in potentiostatic and galvanostatic conditions. Different treatments, involving temperature and polishing control, led to electrodes with different roughness. The electrochemical behavior of the generated gold surface was studied by cyclic voltammetry showing the characteristic response of polycrystalline gold, in contrast with disposable gold electrodes fabricated by screen printing from gold inks. The electrodes were chemically modified through the adsorption of alkanethiols selfassembled monolayers and the coupling of a model protein. Both reactions were followed by cyclic voltammetry and Electrochemical Impedance Spectroscopy (EIS). The electrodes have shown high reproducibility in their electrochemical behavior as well as in their modifications.

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Development of disposable low density screen-printed electrode arrays for simultaneous electrochemical measurements of the hybridisation reaction

Cited by 57 publications

References 19 publications

Designs of Enterobacteriaceae Lac Z Gene DNA Gold Screen Printed Biosensors

Designs of Enterobacteriaceae Lac Z Gene DNA Gold Screen Printed Biosensors

Screen-Printed Electrode Modified With Silver Hexacy Anoferrate-Nafion® for Ascorbic Acid Determination

Disposable Gold Electrode Array for Simultaneous Electrochemical Studies

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