Development of DNA vaccines encoding ribosomal proteins (RplL and RpsA) against Nocardia seriolae infection in fish

Chen, Jianlin; Chen, Zhenwei; Wang, Wenji; Hou, Sen; Cai, Jia; Xu, Liqun; Lu, Yue

doi:10.1016/j.fsi.2019.12.014

Cited by 35 publications

(17 citation statements)

References 38 publications

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“…The results of PCR and RT-PCR demonstrated that the Hrp1 and IL-34 genes were only found in the muscle tissue of fish immunized with pcHrp1, pcIL-34 or pcHrp1+pcIL-34, indicating that the recombinant DNA plasmids could avoid local degradation and uptake by the muscle cells at the injection site, resulting in expression at transcription levels. Our results are in agreement with studies investigated in other fish species, including channel catfish [23], orange-spotted grouper [47], snakehead fish [16,66], and the Japanese flounder [11,67]. It is reported that cell membrane permeability and susceptibility of foreign DNA to host enzymes is one of the pivotal factors to evaluate the efficiency of the cellular uptake of DNA vaccines [68]; hence, further studies are required to estimate the efficiency of the cellular uptake of pcIL-34, pcHrp1 and pcHrp1+pcIL-34.…”

Section: Discussionsupporting

confidence: 93%

“…In the present study, largemouth bass were vaccinated with 25 μg of plasmid DNA, the same dose of plasmid DNA was applied in various studies of DNA vaccine development against viral and bacterial diseases in fish such as infectious pancreatic necrosis virus [ 90 ], spring viremia of carp virus [ 91 ], red seabream iridovirus [ 92 ], Mycobacterium marinum [ 93 ], and N. seriolae infection [ 66 , 94 ]. In our previous study, largemouth bass immunized with 10 μg of plasmid DNA encoding Resuscitation promoting factor B (RpfB) and Low molecular weight T-cell antigen (TB8.4) could not provide effective protection with an RPS value of 6.25% and 39% respectively.…”

Section: Discussionmentioning

confidence: 99%

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Interleukin 34 Serves as a Novel Molecular Adjuvant against Nocardia Seriolae Infection in Largemouth Bass (Micropterus Salmoides)

2020

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DNA vaccines have been widely employed in controlling viral and bacterial infections in mammals and teleost fish. Co-injection of molecular adjuvants, including chemokines, cytokines, and immune co-stimulatory molecules, is one of the potential strategies used to improve DNA vaccine efficacy. In mammals and teleost fish, interleukin-34 (IL-34) had been described as a multifunctional cytokine and its immunological role had been confirmed; however, the adjuvant capacity of IL-34 remains to be elucidated. In this study, IL-34 was identified in largemouth bass. A recombinant plasmid of IL-34 (pcIL-34) was constructed and co-administered with a DNA vaccine encoding hypoxic response protein 1 (Hrp1; pcHrp1) to evaluate the adjuvant capacity of pcIL-34 against Nocardia seriolae infection. Our results indicated that pcIL-34 co-injected with pcHrp1 not only triggered innate immunity and a specific antibody response, but also enhanced the mRNA expression level of immune-related genes encoding for cytokines, chemokines, and humoral and cell-mediated immunity. Moreover, pcIL-34 enhanced the protection of pcHrp1 against N. seriolae challenge and conferred the relative percent survival of 82.14%. Collectively, IL-34 is a promising adjuvant in a DNA vaccine against nocardiosis in fish.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Interleukin 34 Serves as a Novel Molecular Adjuvant against Nocardia Seriolae Infection in Largemouth Bass (Micropterus Salmoides)

2020

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“…Does phosphorylation of GhRPS6 affect its function? Ribosomal biogenesis involves the manufacture and processing of ribosomal RNAs, the biosynthesis of ribosomal proteins, and the transport of ribosomal proteins to the nucleolus to assemble the preribosomal particles [40][41][42][43][44][45][46][47][48]. Existing literature shows that ribosomal proteins are involved in various physiological functions of cells and have been reported to regulate plant growth [41].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Analysis of Ribosomal Protein GhRPS6 and Its Role in Cotton Verticillium Wilt Resistance

Zhu

Zhang

Zhou

et al. 2021

IJMS

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Verticillium wilt is threatening the world’s cotton production. The pathogenic fungus Verticillium dahliae can survive in the soil in the form of microsclerotia for a long time, colonize through the root of cotton, and invade into vascular bundles, causing yellowing and wilting of cotton leaves, and in serious cases, leading to plant death. Breeding resistant varieties is the most economical and effective method to control Verticillium wilt. In previous studies, proteomic analysis was carried out on different cotton varieties inoculated with V. dahliae strain Vd080. It was found that GhRPS6 was phosphorylated after inoculation, and the phosphorylation level in resistant cultivars was 1.5 times than that in susceptible cultivars. In this study, knockdown of GhRPS6 expression results in the reduction of SA and JA content, and suppresses a series of defensive response, enhancing cotton plants susceptibility to V. dahliae. Overexpression in Arabidopsis thaliana transgenic plants was found to be more resistant to V. dahliae. Further, serines at 237 and 240 were mutated to phenylalanine, respectively and jointly. The transgenic Arabidopsis plants demonstrated that seri-237 compromised the plant resistance to V. dahliae. Subcellular localization in Nicotiana benthamiana showed that GhRPS6 was localized in the nucleus. Additionally, the pathogen inoculation and phosphorylation site mutation did not change its localization. These results indicate that GhRPS6 is a potential molecular target for improving resistance to Verticillium wilt in cotton. This lays a foundation for breeding disease-resistant varieties.

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“…From these, a DNA vaccine encoding the FHA gene against fish nocardiosis was developed showing promising results for the hybrid snakehead challenged with N. seriolae. Other DNA vaccines encoding ribosomal proteins (RplL and RpsA) have been developed and successfully tested against Nocardia seriolae infection in fish (Chen et al 2020).…”

Section: Mycobacterium Sppmentioning

confidence: 99%

“…Other DNA vaccines encoding ribosomal proteins (RplL and RpsA) have been developed and successfully tested against Nocardia seriolae infection in fish (Chen et al . 2020).…”

Section: Granulomatous Infections In Fishmentioning

confidence: 99%

Granulomatosis in fish aquaculture: a mini review

Martínez‐Lara

Martínez‐Porchas

Gollas‐Galván

et al. 2020

Reviews in Aquaculture

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Bacterial infections represent the greatest threat for fish culture, especially those causing granulomatous processes. Although there are experimental models used to elucidate the mechanisms of pathogenicity in granulomatous processes, most of these aimed to understand the infection in superior vertebrates like humans; therefore, there is still an urgent need to expand the number of infection models focused on aquaculture. Granulomatous infections are difficult to detect in early stages due to the lack of clinical signs and because these clinical signs have a great similarity to those generated by other pathogenic bacteria. Besides, there is no evidence or reports of granulomatous co-infections in aquaculture environments which is a plausible scenario either in culture systems or in the wild environment; co-infections could interfere with a correct diagnosis of any granulomatous disease, as reported for granulomatous infections in terrestrial species. This revision aims to highlight the state of the art in the knowledge of pathogenic bacteria associated with granulomatosis in fish aquaculture as well as analysing this information.

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Development of DNA vaccines encoding ribosomal proteins (RplL and RpsA) against Nocardia seriolae infection in fish

Cited by 35 publications

References 38 publications

Interleukin 34 Serves as a Novel Molecular Adjuvant against Nocardia Seriolae Infection in Largemouth Bass (Micropterus Salmoides)

Interleukin 34 Serves as a Novel Molecular Adjuvant against Nocardia Seriolae Infection in Largemouth Bass (Micropterus Salmoides)

Genome-Wide Analysis of Ribosomal Protein GhRPS6 and Its Role in Cotton Verticillium Wilt Resistance

Granulomatosis in fish aquaculture: a mini review

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