Development of dsRNA as a Sustainable Bioinsecticide: From Laboratory to Field

Rodrigues, Thais B.; Sridharan, Krishnakumar; Manley, Brian; Cunningham, D; Narva, Kenneth E.

doi:10.1021/bk-2021-1390.ch005

Cited by 14 publications

(8 citation statements)

References 43 publications

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“…An insect receiving considerable focus for a SIGS-based commercial exploitation, due to its high sensitivity to RNAi effects, is the Colorado potato beetle, Leptinotarsa decemlineata . Recently, Syngenta and Greenlight Biosciences have successfully concluded independent field trials showing a better performance of dsRNA-treated plants to control the Colorado potato beetle ( 13 , 14 ). In addition to SIGS-based plant protection products, RNAi-based products for the protection of pollinators are moving to the market.…”

Section: Moving To the Marketmentioning

confidence: 99%

RNAi-Based Biocontrol Products: Market Status, Regulatory Aspects, and Risk Assessment

et al. 2022

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Section: Moving To the Marketmentioning

confidence: 99%

RNAi-Based Biocontrol Products: Market Status, Regulatory Aspects, and Risk Assessment

et al. 2022

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“…Insecticidal gene silencing by RNA interference (RNAi) through foliar delivery of a double stranded RNA (dsRNA) is a new mode of action biopesticide against L. decemlineata (Rodrigues et al 2021a). Upon ingestion, the dsRNA activates a post-transcriptional gene silencing process (RNAi) that results in targeting and degradation of Journal of Economic Entomology, 116(4), 2023Entomology, 116(4), , 1352Entomology, 116(4), -1359 https://doi.org/10.1093/jee/toad095 Advance Access Publication Date: 1 June 2023 transcripts matching the dsRNA sequence (Yoon et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Lack of fitness costs in dsRNA-resistant Leptinotarsa decemlineata ([Coleoptera]: [Chrysomelidae])

Pinto

Santos

Bortoli

et al. 2023

Journal of Economic Entomology

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The Colorado potato beetle, Leptinotarsa decemlineata (Say) ([Coleoptera]: [Chrysomelidae]), is the most important defoliator of solanaceous plants worldwide. This insect displays a notorious ability in adapting to biological and synthetic insecticides, although in some cases this adaptation carries relevant fitness costs. Insecticidal gene silencing by RNA interference is a novel mode of action pesticide against L. decemlineata that is activated by ingestion of a double stranded RNA (dsRNA) targeting a vital L. decemlineata gene. We previously reported laboratory selection of a > 11,000-fold resistant strain of L. decemlineata to a dsRNA delivered topically to potato leaves. In this work, we tested the existence of fitness costs in this dsRNA-resistant colony by comparing biological parameters to the parental strain and an additional susceptible reference strain. Biological parameters included length of egg incubation period, number of eggs per clutch, egg viability, larval viability, length of larval and pupal periods, adult emergence, number of eggs laid per day, sex ratio, and adult longevity. Comparisons between the 3 beetle strains detected no fitness costs associated with resistance to dsRNA. This information is important to guide effective insect resistance management plans for dsRNA insecticides against L. decemlineata applied topically to potato leaves.

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“…Targeting insect pests, especially beetles, with double-stranded ribonucleic acid (dsRNA) molecules has shown great promise as an alternative to chemical insecticides ( 9 – 11 ). Exogenous dsRNA activates the RNA interference (RNAi) pathway, which is a conserved and innate biological defense mechanism in eukaryotic organisms against viruses and transposons via post-transcriptional gene silencing ( 12 ).…”

Section: Introductionmentioning

confidence: 99%

“…Exogenous dsRNA activates the RNA interference (RNAi) pathway, which is a conserved and innate biological defense mechanism in eukaryotic organisms against viruses and transposons via post-transcriptional gene silencing ( 12 ). Unlike common chemical insecticides, dsRNAs are designed to target mRNA sequences unique to the target pest because of the necessity to have high sequence fidelity for gene silencing to occur ( 9 ). Furthermore, dsRNA has a low potential for persistence in the environment, including soil, sediment, and surface water compartments, because of its instability in environmental conditions and rapid microbial degradation ( 9 , 13 , 14 ).…”

Section: Introductionmentioning

confidence: 99%

“…Unlike common chemical insecticides, dsRNAs are designed to target mRNA sequences unique to the target pest because of the necessity to have high sequence fidelity for gene silencing to occur ( 9 ). Furthermore, dsRNA has a low potential for persistence in the environment, including soil, sediment, and surface water compartments, because of its instability in environmental conditions and rapid microbial degradation ( 9 , 13 , 14 ).…”

Section: Introductionmentioning

confidence: 99%

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Gene silencing in adult Popillia japonica through feeding of double‐stranded RNA (dsRNA) complexed with branched amphiphilic peptide capsules (BAPCs)

Carroll,

Kunte,

McGraw

et al. 2023

Front. Insect Sci.

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Gene silencing by feeding double-stranded (dsRNA) holds promise as a novel pest management strategy. Nonetheless, degradation of dsRNA in the environment and within the insect gut, as well as inefficient systemic delivery are major limitations to applying this strategy. Branched amphiphilic peptide capsules (BAPCs) complexed with dsRNA have been used to successfully target genes outside and inside the gut epithelium upon ingestion. This suggests that BAPCs can protect dsRNA from degradation in the gut environment and successfully shuttle it across gut epithelium. In this study, our objectives were to 1) Determine whether feeding on BAPC-dsRNA complexes targeting a putative peritrophin gene of P. japonica would result in the suppression of gut peritrophin synthesis, and 2) gain insight into the cellular uptake mechanisms and transport of BAPC-dsRNA complexes across the larval midgut of P. japonica. Our results suggest that BAPC-dsRNA complexes are readily taken up by the midgut epithelium, and treatment of the tissue with endocytosis inhibitors effectively suppresses intracellular transport. Further, assessment of gene expression in BAPC- peritrophin dsRNA fed beetles demonstrated significant downregulation in mRNA levels relative to control and/or dsRNA alone. Our results demonstrated that BAPCs increase the efficacy of gene knockdown relative to dsRNA alone in P. japonica adults. To our knowledge, this is the first report on nanoparticle-mediated dsRNA delivery through feeding in P. japonica.

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Development of dsRNA as a Sustainable Bioinsecticide: From Laboratory to Field

Cited by 14 publications

References 43 publications

RNAi-Based Biocontrol Products: Market Status, Regulatory Aspects, and Risk Assessment

RNAi-Based Biocontrol Products: Market Status, Regulatory Aspects, and Risk Assessment

Lack of fitness costs in dsRNA-resistant Leptinotarsa decemlineata ([Coleoptera]: [Chrysomelidae])

Gene silencing in adult Popillia japonica through feeding of double‐stranded RNA (dsRNA) complexed with branched amphiphilic peptide capsules (BAPCs)

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