This study formulates a method to calculate the collapse time of wood members exposed to fire heating by considering the influence of temperature and moisture content. Moisture content affects the mechanical properties of wood members adversely during fire heating because of an increase in moisture content by water transfer and thermal softening at high temperature; however, the impact on mechanical properties of wood members during fire heating has been previously evaluated with respect to temperature alone. Consequently, this study formulates the calculation of the buckling time of a Japanese zelkova column under two conditions of initial moisture content (13.4% and 30.4%) and compares it with previous experimental results. This study showed that moisture content increased from 13.4% to 20.0% and from 30.4% to 39.1% at most for the two conditions, respectively, during fire heating. The buckling time could be predicted with good accuracy considering the moisture content dependence of Young's modulus. Although the moment of inertia was 1.67 times at 82 minutes when the column buckled in the experiment when the moisture content was high, the buckling time was only 3.5 min later than at low moisture content. Therefore, this calculation results show high initial moisture content increased in the remaining cross-sectional area and decreased Young's modulus significantly.