Development of Early Infection Structures of Puccinia recondita f. sp. tritici in Non‐host Cereal Species

Abstract

The development of infection structures, derived from urediospores of Puccinia recondita f.sp. trilici in nearisogenic lines of susceptible and resistant wheat, and in non-hosts (viz. maize, oat, sorghum and barley), was examined by fluorescence microscopy and scanning electron microscopy (SEM). The infection structure formation on and in five cereal species follows a similar pattern. In sorghum, fungal development is arrested at the stage of substomatal vesicle formation, while, in maize, most fungal structures collapse during the stage of primary hypha development. By contrast, in wheat, barley and oat, the fungus forms many branched infection hyphae and haustorial mother cells.

Temporal Gene Expression Profiling of the Wheat Leaf Rust Pathosystem Using cDNA Microarray Reveals Differences in Compatible and Incompatible Defence Pathways

“…Those authors have studied the infection process of P. triticinia on susceptible (Thatcher) and resistant wheat lines (RL6040 which contains the Lr19 leaf rust resistance gene and RL6043 which contains the Lr21 leaf rust resistance gene) of wheat and no significant differences were observed between the three lines during these early infection events. Moreover, very similar results were obtained during P. triticinia infection of nonhost species such as maize, oat, sorghum, and barley [ 39 ]. Based on these studies, it is likely that the growth of the two races of P. triticinia used on RL6003 in this study invoke very similar responses during the early infection stages as that of the P. triticinia races used by Hu and Rijkenberg on other resistant wheat lines [ 9 ].…”

Network Analysis Combining Proteomics and Metabolomics Reveals New Insights Into Early Responses of Eucalyptus grandis During Rust Infection

“…In a similar investigation of Puccinia recondita sp. tritici in non-host species, Hu and Rijkenberg (1998) were also unable to detect fungal development after substomatal vesicle formation in sorghum. In addition, only fragments of hyphal structures were observed, and the pathogen had no progression in R3 genotype at 24 hai, at the same time-point A. psidii produced haustorium in S4 plants.…”

“…In general, the stages of rust infection can be categorized into temporal phases corresponding to fungal development in which plants recognize specialized structures and activate defense responses ( Hu and Rijkenberg, 1998 ). Therefore, light microscopy analysis can be a useful method to elucidate the timeframe in which the pathogen growth ceases in resistant plants ( Hu and Rijkenberg, 1998 ; Xavier et al, 2001 ; Ayliffe et al, 2011 ; Zhang et al, 2011 ) and progress in susceptible tissues. We noticed that MF1 urediniospores germinated at 3 hai and facilitated appressorium formation at 6 hai in both R3 and S4 genotypes.…”

“…Enzymes and related precursors and products, which are not present in "R" and "S" protein modules, were removed. defense responses (Hu and Rijkenberg, 1998). Therefore, light microscopy analysis can be a useful method to elucidate the timeframe in which the pathogen growth ceases in resistant plants (Hu and Rijkenberg, 1998;Xavier et al, 2001;Ayliffe et al, 2011;Zhang et al, 2011) and progress in susceptible tissues.…”

Data_Sheet_1.pdf

“…defense responses (Hu and Rijkenberg, 1998). Therefore, light microscopy analysis can be a useful method to elucidate the timeframe in which the pathogen growth ceases in resistant plants (Hu and Rijkenberg, 1998;Xavier et al, 2001;Ayliffe et al, 2011;Zhang et al, 2011) and progress in susceptible tissues. We noticed that MF1 urediniospores germinated at 3 hai and facilitated appressorium formation at 6 hai in both R3 and S4 genotypes.…”