Development of electrochemical genosensor for MYCN oncogene detection using rhodamine B as electroactive label

Silva, Thalles Douglas Souza e; Castro, Ana Cristina Honorato de; Rodovalho, Vinícius de Rezende; Madurro, João M.; Madurro, Ana G. B.

doi:10.1007/s10008-016-3326-0

Cited by 13 publications

(2 citation statements)

References 51 publications

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“…(3) Electrochemical method: The electrochemical signal generated byMYCN was detected by electrochemical analysis. Compared with traditional analytical methods, electrochemical methods have the advantages of a simple operation, short time consumption, good specificity, high sensitivity and low experimental cost [108]. Therefore, among these detection methods, real-time quantitative PCR is the most suitable method for clinical use.…”

Section: Circulating-free Mycn Detection Methods and Problems In Clin...mentioning

confidence: 99%

A systematic review of circulating-free MYCN amplification status as a noninvasive and prominent prognostic and recurrence monitoring indicator for neuroblastoma

Zhang

Liang

Zhang

et al. 2022

Preprint

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MYCN gene plays an important role in neuroblastoma cell growth, apoptosis, differentiation, tumor infiltration, metastasis, and angiogenesis. MYCN gene amplification status in tumor tissue is one of the important clinical indicators of neuroblastoma risk. However, there are several problems in detecting the MYCN gene in tumor tissues, such as complex procedures in detection approaches, high detection costs, and difficulty in obtaining tumor tissues. Studies have shown that circulating-free MYCN gene amplification in neuroblastoma is consistent with that in tumor tissue. Circulating-free MYCN gene amplification has the advantages of high sensitivity, high specificity, simple operation, and non-invasiveness as an indicator for prognosis and recurrence of neuroblastoma. Therefore, the application and future development of circulating-free MYCN gene amplification status in the prognosis and recurrence monitoring of neuroblastoma was examined in this article.

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Section: Circulating-free Mycn Detection Methods and Problems In Clin...mentioning

confidence: 99%

A systematic review of circulating-free MYCN amplification status as a noninvasive and prominent prognostic and recurrence monitoring indicator for neuroblastoma

Zhang

Liang

Zhang

et al. 2022

Preprint

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“…There is a great variety of materials that can be used in the construction of polymer lms, being fundamental that they allow the immobilization of active species, increase the transfer of electrons and improve the selectivity through the blocking of interferents [10]. Several electrochemical biosensors that use the genetic material for the detection of diseases can be found in the literature, for example for meningococcal meningitis [20], Epstein-Barr virus [21], the oncogenic marker MYCN [22], hepatitis C virus [23,24], Zika virus [25] and Mycobacterium leprae [26]. Srisomwat and collaborators [3] described a device based on 3D micro uidic paper (μPAD) for detecting HBV DNA.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Biosensor for Sensitive Detection of Hepatitis B in Human Plasma

Castro

Kochi

Flauzino

et al. 2022

Appl Biochem Biotechnol

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In this work we report the construction of an electrochemical device for molecular diagnosis of hepatitis B virus in the blood plasma of infected patients, using graphite electrodes functionalized with poly(4aminophenol) and sensitized with speci c DNA probe. The recognition of genomic DNA was evaluated by electrochemical techniques (DPV and EIS) and scanning electron microscopy. The genosensor was e cient in detecting genomic DNA with a linear range from 1.176 μg.ml -1 to 4.825 μg.ml -1 and detection limit of 35.69 ng.ml -1 (4.63 UI.ml -1 or 25.93 copies.ml -1 ), which is better than 10.00 UI.ml-1 limit of reference method, real-time PCR, used in point of care. EIS analysis shows that the genosensor resistence increased exponentially with the concentration of the genomic DNA target. The developed platform has inherent advantages to its applicability in real samples, such as good sensitivity, selectivity, and low sample volume, being interesting for application in diagnosis of hepatitis B virus in blood plasma.

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Novel electrochemical platform based on copolymer poly(aniline-4-aminophenol) for application in immunosensor for thyroid hormones

Segatto

Soler

Oliveira

et al. 2020

J Solid State Electrochem

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Development of electrochemical genosensor for MYCN oncogene detection using rhodamine B as electroactive label

Cited by 13 publications

References 51 publications

A systematic review of circulating-free MYCN amplification status as a noninvasive and prominent prognostic and recurrence monitoring indicator for neuroblastoma

A systematic review of circulating-free MYCN amplification status as a noninvasive and prominent prognostic and recurrence monitoring indicator for neuroblastoma

Electrochemical Biosensor for Sensitive Detection of Hepatitis B in Human Plasma

Novel electrochemical platform based on copolymer poly(aniline-4-aminophenol) for application in immunosensor for thyroid hormones

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