Development of Electrochemical HRP-MWCNT-Based Screen-Printed Biosensor for the Determination of Phenolic Compounds in Effluent from Washing Coffee Beans

Duarte, Raquel Reis; Giarola, Juliana de Fátima; Silva, Daniela Nunes da; Saczk, Adelir Aparecida; Tarley, César Ricardo Teixeira; Ribeiro, Emerson Schwingel; Pereira, Arnaldo César

doi:10.21577/1984-6835.20200129

Cited by 4 publications

(5 citation statements)

References 37 publications

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“…It is possible to notice in Figure 3d that a thin layer of enzyme was formed on the surface of the support after HRP immobilization. A similar distribution of the enzyme on the surface of MWCNTs is shown in studies conducted by Duarte et al [41], confirming our assumptions on MWCNTs' suitability as supports for HRP. Both pristine materials and the obtained biocatalytic systems after HRP immobilization were also analyzed using scanning electron microscopy (SEM, Figure 3) and by determining changes in the particle size of the support before and after immobilization (Table 1).…”

Section: Physicochemical Characterization Of Materials Before and Aft...supporting

confidence: 92%

“…It is possible to notice in Figure 3d that a thin layer of enzyme was formed on the surface of the support after HRP immobilization. A similar distribution of the enzyme on the surface of MWCNTs is shown in studies conducted by Duarte et al [41], confirming our assumptions on MWCNTs' suitability as supports for HRP. 3e presents an SEM image of the pristine chitosan-coated magnetic nanoparticles doped with selenium ions.…”

Section: Physicochemical Characterization Of Materials Before and Aft...supporting

confidence: 92%

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Support Materials of Organic and Inorganic Origin as Platforms for Horseradish Peroxidase Immobilization: Comparison Study for High Stability and Activity Recovery

Bilal,

Degorska,

Szada

et al. 2024

Molecules

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In the presented study, a variety of hybrid and single nanomaterials of various origins were tested as novel platforms for horseradish peroxidase immobilization. A thorough characterization was performed to establish the suitability of the support materials for immobilization, as well as the activity and stability retention of the biocatalysts, which were analyzed and discussed. The physicochemical characterization of the obtained systems proved successful enzyme deposition on all the presented materials. The immobilization of horseradish peroxidase on all the tested supports occurred with an efficiency above 70%. However, for multi-walled carbon nanotubes and hybrids made of chitosan, magnetic nanoparticles, and selenium ions, it reached up to 90%. For these materials, the immobilization yield exceeded 80%, resulting in high amounts of immobilized enzymes. The produced system showed the same optimal pH and temperature conditions as free enzymes; however, over a wider range of conditions, the immobilized enzymes showed activity of over 50%. Finally, a reusability study and storage stability tests showed that horseradish peroxidase immobilized on a hybrid made of chitosan, magnetic nanoparticles, and selenium ions retained around 80% of its initial activity after 10 repeated catalytic cycles and after 20 days of storage. Of all the tested materials, the most favorable for immobilization was the above-mentioned chitosan-based hybrid material. The selenium additive present in the discussed material gives it supplementary properties that increase the immobilization yield of the enzyme and improve enzyme stability. The obtained results confirm the applicability of these nanomaterials as useful platforms for enzyme immobilization in the contemplation of the structural stability of an enzyme and the high catalytic activity of fabricated biocatalysts.

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Section: Physicochemical Characterization Of Materials Before and Aft...supporting

confidence: 92%

“…It is possible to notice in Figure 3d that a thin layer of enzyme was formed on the surface of the support after HRP immobilization. A similar distribution of the enzyme on the surface of MWCNTs is shown in studies conducted by Duarte et al [41], confirming our assumptions on MWCNTs' suitability as supports for HRP. 3e presents an SEM image of the pristine chitosan-coated magnetic nanoparticles doped with selenium ions.…”

Section: Physicochemical Characterization Of Materials Before and Aft...supporting

confidence: 92%

Support Materials of Organic and Inorganic Origin as Platforms for Horseradish Peroxidase Immobilization: Comparison Study for High Stability and Activity Recovery

Bilal,

Degorska,

Szada

et al. 2024

Molecules

View full text Add to dashboard Cite

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“…The values obtained for HQ, CAT, and RES were (0.42 ± 0.05) mM, (2.20 ± 0.07) mM, and (0.29 ± 0.02) mM, respectively. These values are in the range of values (0.11-3.2 mM) previously reported by other authors [49], ensuring that the immobilized enzyme retains its essential catalytic properties. In the case of CGA, the Eadie-Hofstee plot shows two well-defined slopes.…”

Section: Chronoamperometric Response Of Phenolic Compoundssupporting

confidence: 78%

“…Table 2 collects the average values of the electroanalytical parameters obtained for the developed biosensor and compares them with those reported for others containing HRP regarding HQ, CGA, CAT, and RES. Even though the average concentration of polyphenolic compounds in plant extracts can vary significantly depending on several factors (plant species, part of the plant used, extraction method, and plant growth conditions, among others), the results obtained demonstrate the excellent performance of our bioelectrode, considering values previously reported for yerba mate and green coffee extracts, 13.2 and 0.04 µM, respectively [49].…”

Section: Chronoamperometric Response Of Phenolic Compoundssupporting

confidence: 58%

Immobilization of Horseradish Peroxidase onto Montmorillonite/Glucosamine–Chitosan Composite for Electrochemical Biosensing of Polyphenols

Piccoli,

Gulotta,

Montenegro

et al. 2024

Biosensors

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Glucosamine–chitosan synthesized by the Maillard reaction was combined with montmorillonite to obtain a nanohybrid composite to immobilize horseradish peroxidase. The material combines the advantageous properties of clay with those of the chitosan derivative; has improved water solubility and reduced molecular weight and viscosity; involves an eco-friendly synthesis; and exhibits ion exchange capacity, good adhesiveness, and a large specific surface area for enzyme adsorption. The physicochemical characteristics of the composite were analyzed by infrared spectroscopy and X-ray diffraction to determine clay–polycation interactions. The electrochemical response of the different polyphenols to glassy carbon electrodes modified with the composite was evaluated by cyclic voltammetry. The sensitivity and detection limit values obtained with the biosensor toward hydroquinone, chlorogenic acid, catechol, and resorcinol are (1.6 ± 0.2) × 102 µA mM−1 and (74 ± 8) nM; (1.2 ± 0.1) × 102 µA mM−1 and (26 ± 3) nM; (16 ± 2) µA mM−1 and (0.74 ± 0.09) μM; and (3.7± 0.3) µA mM−1 and (3.3 ± 0.2) μM, respectively. The biosensor was applied to quantify polyphenols in pennyroyal and lemon verbena extracts.

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“…[41,98,100,102,[107][108][109][118][119][120][121][122][123][124][125][126][127][128][129][130][131][134][135][136][137][138][139][140][141][142][143][144][145][146][147][148][149][150][151] Selectivity[102,106,109,110,113,116,[120][121][122][123]125,129,…”

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Water Quality Carbon Nanotube-Based Sensors Technological Barriers and Late Research Trends: A Bibliometric Analysis

et al. 2022

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Water is the key element that defines and individualizes our planet. Relative to body weight, water represents 70% or more for the majority of all species on Earth. Taking care of water as a whole is equivalent with taking care of the entire biodiversity or the whole of humanity itself. Water quality is becoming an increasingly important component of terrestrial life, hence intensive work is being conducted to develop sensors for detecting contaminants and assessing water quality and characteristics. Our bibliometric analysis is focused on water quality sensors based on carbon nanotubes and highlights the most important objectives and achievements of researchers in recent years. Due to important measurement characteristics such as sensitivity and selectivity, or low detection limit and linearity, up to the ability to measure water properties, including detection of heavy metal content or the presence of persistent organic compounds, carbon nanotube (CNT) sensors, taking advantage of available nanotechnologies, are becoming increasingly attractive. The conducted bibliometric analysis creates a visual, more efficient keystones mapping. CNT sensors can be integrated into an inexpensive real-time monitoring data acquisition system as an alternative for classical expensive and time-consuming offline water quality monitoring. The conducted bibliometric analysis reveals all connections and maps all the results in this water quality CNT sensors research field and gives a perspective on the approached methods on this specific type of sensor. Finally, challenges related to integration of other trends that have been used and proven to be valuable in the field of other sensor types and capable to contribute to the development (and outlook) for future new configurations that will undoubtedly emerge are presented.

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Development of Electrochemical HRP-MWCNT-Based Screen-Printed Biosensor for the Determination of Phenolic Compounds in Effluent from Washing Coffee Beans

Cited by 4 publications

References 37 publications

Support Materials of Organic and Inorganic Origin as Platforms for Horseradish Peroxidase Immobilization: Comparison Study for High Stability and Activity Recovery

Support Materials of Organic and Inorganic Origin as Platforms for Horseradish Peroxidase Immobilization: Comparison Study for High Stability and Activity Recovery

Immobilization of Horseradish Peroxidase onto Montmorillonite/Glucosamine–Chitosan Composite for Electrochemical Biosensing of Polyphenols

Water Quality Carbon Nanotube-Based Sensors Technological Barriers and Late Research Trends: A Bibliometric Analysis

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