Development of electrospun, biomimetic tympanic membrane implants with tunable mechanical and oscillatory properties for myringoplasty

Benecke, Lukas; Chen, Zhaoyu; Zeidler-Rentzsch, Ines; Witzleben, Max von; Bornitz, Matthias; Zahnert, Thomas; Neudert, Marcus; Cherif, Chokri; Aibibu, Dilbar

doi:10.1039/d1bm01815a

Cited by 10 publications

(3 citation statements)

References 50 publications

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“…Furthermore, exact knowledge of the TM anatomy is of great interest in certain biomedical fields of research, which includes the modeling of the middle ear function (4). The morphology of the TM also plays a role in the development of TM replacement materials (5,6).…”

Section: Introductionmentioning

confidence: 99%

In Vivo Thickness of the Healthy Tympanic Membrane Determined by Optical Coherence Tomography

Morgenstern,

Kreusch,

Golde

et al. 2024

Otol Neurotol

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Objective Tympanic membrane (TM) thickness is an important parameter for differentiation between a healthy and a pathologic TM. Furthermore, it is needed for modeling the middle ear function. Endoscopic optical coherence tomography (eOCT) provides the opportunity to measure the TM thickness of the entire TM in vivo. Materials and methods A total of 27 healthy ears were examined by eOCT. The system uses a light source with a central wavelength of 1,300 nm. The endoscope with an outer diameter of 3.5 mm provides a field of view of 10 mm and a working distance of 10 mm. Thickness measurements were carried out at 8 points on the TM. Additionally, the existing literature was analyzed, and a mean TM thickness value was determined. Results The mean thickness of the TM over all measurement points of the pars tensa was 120.2 μm, and the pars flaccida was significantly thicker with a mean thickness of 177.9 μm. Beyond that, there were no significant differences between the single quadrants. The mean TM thickness in the literature was 88.8 μm. Discussion EOCT provides the possibility for in vivo thickness determination of the TM. The mean thickness seems to be higher than in the previous studies, which were mostly carried out ex vivo. Our study takes the three-dimensional refraction into account and provides a method for the refraction correction.

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Section: Introductionmentioning

confidence: 99%

In Vivo Thickness of the Healthy Tympanic Membrane Determined by Optical Coherence Tomography

Morgenstern,

Kreusch,

Golde

et al. 2024

Otol Neurotol

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“…However, with the growing prominence of tissue engineering, several biomaterial- and biofabrication-based strategies are being investigated for reconstructing the perforated TM with a superior biological and mechano-acoustical response [ [19] , [20] , [21] , [22] , [23] ]. A wide range of natural and synthetic polymers, spanning from silk fibroin [ 24 , 25 ], alginate [ 26 ], gelatin [ 27 , 28 ] to poly(ε-caprolactone) [ [29] , [30] , [31] , [32] ], and poly(ethylene oxide terephthalate)/poly(butylene terephthalate) (PEOT/PBT) [ 19 , [33] , [34] , [35] , [36] ] have been studied for this purpose in combination with biofabrication techniques, such as electrospinning (ES) [ 19 , [30] , [31] , [32] , [33] , [34] , [35] , [36] , [37] , [38] , [39] , [40] ] and additive manufacturing [ [19] , [20] , [21] , 28 , 29 , 33 , 41 , 42 ]. The ability to create nanofibrous electrospun patches with dimensions similar to those of native collagen fibrils in the extracellular matrix [ 43 ] supports as a promising approach for reconstructing CSOM-induced injuries using tissue-engineered scaffolds.…”

Section: Introductionmentioning

confidence: 99%

Tunable ciprofloxacin delivery through personalized electrospun patches for tympanic membrane perforations

Anand,

Fusco,

Günday

et al. 2024

Bioactive Materials

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“…Native TMs are durable but susceptible to damage and rupture due to the infection or injury. Artificial TMs should withstand normal physiological conditions and show resistance to degradation over time [ 9 , 10 ]…”

Section: Introductionmentioning

confidence: 99%

Polyacrylamide/Gel-Based Self-Healing Artificial Tympanic Membrane for Drug Delivery of Otitis Treatment

Kim,

Goo,

Karima

et al. 2024

Biomater Res

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One of the bacterial infections caused by tympanic membrane perforation is otitis media (OM). Middle ear inflammation causes continuous pain and can be accompanied by aftereffects such as facial nerve paralysis if repeated chronically. Therefore, it is necessary to develop an artificial tympanic membrane (TM) that can effectively regenerate the eardrum due to the easy implantation and removal of OM inflammation. In this study, we synthesized hydrogel by mixing gelatin and polyacrylamide. Cefuroxime sodium salt was then incorporated into this hydrogel to both regenerate the TM and treat OM. Cytotoxicity experiments confirmed the biocompatibility of hydrogels equipped with antibiotics, and we conducted drug release and antibacterial experiments to examine continuous drug release. Through experiments, we have verified the excellent biocompatibility, drug release ability, and antibacterial effectiveness of hydrogel. It holds the potential to serve as an effective strategy for treating OM and regenerating TM as a drug delivery substance.

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Development of electrospun, biomimetic tympanic membrane implants with tunable mechanical and oscillatory properties for myringoplasty

Abstract: The mechanical and oscillatory behavior of the biomimetic electrospun SF-PCL TM implants can be tuned by adjusting the solution concentration, the SF-PCL mixing ratio and the electrospinning parameters to achieve comparable properties to human TMs.

Cited by 10 publications

References 50 publications

In Vivo Thickness of the Healthy Tympanic Membrane Determined by Optical Coherence Tomography

In Vivo Thickness of the Healthy Tympanic Membrane Determined by Optical Coherence Tomography

Tunable ciprofloxacin delivery through personalized electrospun patches for tympanic membrane perforations

Polyacrylamide/Gel-Based Self-Healing Artificial Tympanic Membrane for Drug Delivery of Otitis Treatment

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