Development of Excised Larynx

Abstract

The larynx is a complex organ which has a role in a variety of functions such as phonation, breathing, and swallowing. To research these functions, it is widely accepted that in vivo studies provide more anatomically and physiologically relevant findings. However, invasive procedures are generally needed to measure variables such a subglottal pressure, vocal fold tension and stiffness, and cricothyroid muscle stretch. Performing studies using excised larynges is a useful technique which makes it possible to not only measure phonation parameters but control them as well. Early studies using excised larynges mainly focused on controlling specific parameters and mathematical modeling simulations. The use of these studies has helped further research in laryngeal anatomy, imaging techniques, as well as aerodynamic, acoustic, and biomechanical properties. Here, we describe the progress of this research over the past five years. The number of accepted animal models has increased and ideas from excised larynx studies are starting to be applied to treatment methods for laryngeal disorders. These experiments are only valid for an excised situation and must continue to be combined with animal experimentation and clinical observations

Towards a Clinically Applicable Computational Larynx Model

“…Other measuring techniques include acoustic voice measurement and electroglottography (EGG) [18], both of which provide indirect measurements of the process in the larynx. As a consequence, synthetic [19] and ex-vivo [20] larynx models have been established in experimental voice research that provide almost unconfined access to the laryngeal flow region. As an alternative and complementary method, computational models have been developed in recent decades.…”

Impact of Rehydration Following Systemic Dehydration on Vocal Fold Gene Expression

“…Here we have used the rabbit, a validated model for laryngeal histology and surgical training, 8,9 and an established model of systemic dehydration in our previous studies. [10][11][12][13] Through nonhuman in vivo studies, our research group has been building mounting evidence that molecular changes result from dehydration.…”

“…Animal models are, therefore, an attractive substitute allowing a more comprehensive interrogation of the biology underlying the impact of dehydration on the vocal folds. Here we have used the rabbit, a validated model for laryngeal histology and surgical training, 8,9 and an established model of systemic dehydration in our previous studies 10–13 . Through nonhuman in vivo studies, our research group has been building mounting evidence that molecular changes result from dehydration 10,12–18 …”

Microrretalho bipediculado de mucosa cordal em fonomicrocirurgia: estudo experimental em laringes humanas excisadas

“…Desde o século XV há indícios da utilização de laringes excisadas para estudo, desde então esta forma de pesquisa proporcionou avanços importantes na área da laringologia (Garcia e Herbst, 2018). Ferrein 1741 Além de melhorar a compreensão da fisiologia e anatomia, o estudo com laringes excisadas foi importante também para predizer resultados terapêuticos, principalmente na área cirúrgica (Luo, et al, 2018). , 1959;Döllinger et al, 2011).…”