Development of expressed sequence tag-simple sequence repeat markers for genetic characterization and population structure analysis of Praxelis clematidea (Asteraceae)

Abstract

ABSTRACT. Invasive plants tend to spread aggressively in new habitatsand an understanding of their genetic diversity and population structure is useful for their management. In this study, expressed sequence tagsimple sequence repeat (EST-SSR) markers were developed for the invasive plant species Praxelis clematidea (Asteraceae) from 5548 Stevia rebaudiana (Asteraceae) expressed sequence tags (ESTs). A total of 133 microsatellite-containing ESTs (2.4%) were identified, of which 56 (42.1%) were hexanucleotide repeat motifs and 50 (37.6%) were trinucleotide repeat motifs. Of the 24 primer pairs designed from these 133 ESTs, 7 (29.2%) resulted in significant polymorphisms. The number of alleles per locus ranged from 5 to 9. The relatively high genetic diversity (H = 0.2667, I = 0.4212, and P = 100%) of that most genetic variation occurred within populations. A Mantel test suggested that there was significant correlation between genetic distance and geographical distribution (r = 0.3192, P = 0.012). These results further support the transferability of EST-SSR markers between closely related genera of the same family.

Avaliação da citotoxicidade em hemácias de humanos do extrato etanólico de Praxelis clematidea (Griseb.)

“…Hieron ex. Kuntze, e é uma erva perene de vida anual curta, nativa da América do Sul [9,10]. Por ser uma espécie muito adaptável, podendo sobreviver em áreas com diferentes climas, tornou-se invasiva em diversas regiões no mundo, sendo uma ameaça à agricultura, biodiversidade e meio ambiente [11][12].…”