Development of FGF-2-loaded electrospun waterborne polyurethane fibrous membranes for bone regeneration

Abstract

Guided bone regeneration (GBR) membrane has been used to improve functional outcomes for periodontal regeneration. However, few studies have focused on the biomimetic membrane mimicking the vascularization of the periodontal membrane. This study aimed to fabricate waterborne polyurethane (WPU) fibrous membranes loaded fibroblast growth factor-2 (FGF-2) via emulsion electrospinning, which can promote regeneration of periodontal tissue via the vascularization of the biomimetic GBR membrane. A biodegradable WPU was synthesized by using lysine and dimethylpropionic acid as chain extenders according to the rule of green chemical synthesis technology. The WPU fibers with FGF-2 was fabricated via emulsion electrospinning. The results confirmed that controlled properties of the fibrous membrane had been achieved with controlled degradation, suitable mechanical properties and sustained release of the factor. The immunohistochemical expression of angiogenic-related factors was positive, meaning that FGF-2 loaded in fibers can significantly promote cell vascularization. The fiber scaffold loaded FGF-2 has the potential to be used as a functional GBR membrane to promote the formation of extraosseous blood vessels during periodontal repairing.

Fiber configuration determines foreign body response of electrospun scaffolds: in vitro and in vivo assessments

“…Electrospinning is considered the most promising approach to preparing fibrous scaffolds and medical devices. For instance, electrospun scaffolds have been efforted as substitutes to repair and regenerate a variety of tissues and organs in many preclinical trials [11][12][13][14]. With increasing appreciation of FBR to foreign biomaterials, recent studies regarding the assessments of FBR to electrospun scaffolds involved in situ or subcutaneous implantation [15][16][17][18][19][20].…”

Biodegradable and antithrombogenic chitosan/elastin blended polyurethane electrospun membrane for vascular tissue integration

“…Various biomolecules and natural materials have been combined with PU to enhance its biological function. For example, the addition of fibroblast growth factor into PU fibrous electrospun membrane to accelerate cell growth 23 . In another study, PU fiber scaffolds were coated with polyaniline to enhance the scaffold's hydrophilicity for better proliferation of endothelial cells 24 .…”

“…For example, the addition of fibroblast growth factor into PU fibrous electrospun membrane to accelerate cell growth. 23 In another study, PU fiber scaffolds were coated with polyaniline to enhance the scaffold's hydrophilicity for better proliferation of endothelial cells. 24 It was previously reported that CS was also blended with PU to improve cellmaterial interaction.…”

Irisin-loaded electrospun core-shell nanofibers as calvarial periosteum accelerate vascularized bone regeneration by activating the mitochondrial SIRT3 pathway

“…Additionally, it has been demonstrated that the absence of periosteum inevitably leads to nonunion or malunion, whereas the transplantation of periosteum expedites the process of bone regeneration, given the crucial function of periosteum [ 10 ]. Consequently, there has been extensive research on bone tissue engineering, with a focus on developing biomimetic periosteum [ 11 ]. In recent years, electrospinning has emerged as a prominent method for fabricating two-dimensional (2D) nanofibrous membranes and gained great attraction in bone tissue engineering [ 12 ].…”