Development of fish collagen/bioactive glass/chitosan composite nanofibers as a GTR/GBR membrane for inducing periodontal tissue regeneration

Abstract

The development of a guided tissue or bone regeneration (GTR/GBR) membrane with excellent performance has been a major challenge in the biomedical field. The present study was designed to prepare a biomimetic electrospun fish collagen/bioactive glass/chitosan (Col/BG/CS) composite nanofiber membrane and determine its structure, mechanical property, antibacterial activity, and biological effects on human periodontal ligament cells (HPDLCs). The effects of this composite membrane on inducing periodontal tissue regeneration were evaluated using a dog class II furcation defect model. It was found that the composite membrane had a biomimetic structure with good hydrophilicity (the contact angle was 12.83 ± 3°) and a tensile strength of 13.1 ± 0.43 Mpa. Compared to the pure fish collagen membrane, the composite membrane showed some degree of antibacterial activity on Streptococcus mutans. The composite membrane not only enhanced the cell viability and osteogenic gene expression of the HPDLCs, but also promoted the expression of RUNX-2 and OPN protein. Further animal experiments confirmed that the composite membrane was able to promote bone regeneration in the furcation defect of dogs. In conclusion, a biomimetic fish Col/BG/CS composite membrane has been developed in the present study, which can induce tissue regeneration with a certain degree antibacterial activity, providing a basis for potential application as a GTR/GBR membrane.

Development of a novel functionally graded membrane containing boron‐modified bioactive glass nanoparticles for guided bone regeneration

“…Thus, BG content was increased as much as we could, whereas electrospinning was possible. Consequently, mechanical properties of the membranes were in the acceptable range for GBR applications, because the UTS values of similar GBR membranes were reported to be in between 1 and 20 MPa (Requicha et al, 2016;Zhou et al, 2017).…”

“…As cell source for GBR applications, adult stem cells have been widely preferred because of their favourable interaction with organic or inorganic biomaterials (Mota et al, 2012;Requicha et al, 2016;Sheikh et al, 2017;Türkkan et al, 2017;Zhou et al, 2017).…”

Antibacterial Bio-Based Polymers for Cranio-Maxillofacial Regeneration Applications

“…It was reported that chitosan acted synergistically with chlorhexidine or minocycline in collagen membranes for periapical GTR, (J. Wang et al 2016; Kaya et al 2014) showing antibacterial properties as well as biocompatibility and barrier function against Enterococcus faecalis ). On the other hand, chitosan has been widely employed for GBR membranes blended with other natural polymers such as collagen, against S. mutans [42], and PCL and gelatin, against Escherichia coli and Staphylococcus aureus (Figure 1) [37]. As an alternative, Jin et al recently studied a composite-membrane combining chitosan and silver particles, which they hypothesized it could release silver ions from the fibers in a controlled manner in order to achieve enough antibacterial effects without affecting the biocompatibility of the material [30,30,43].…”

Nanomaterials in Craniofacial Tissue Regeneration: A Review

“…Polycaprolactone (PCL) membranes synthesized using the electrospinning technique can be loaded with anti-inflammatory drugs (such as ibuprofen) and growth factors (such as bone morphogenetic protein-2 (BMP-2)) to enhance periodontal regeneration [25]. Biomimetic fish collagen/ bioactive glass/ chitosan composite nanofiber membranes (Col/BG/CS) have good hydrophilicity, higher porosity and surface area promoting cell-cell and cell-matrix interaction, adequate tensile strength, and limited antibacterial properties [26]. Clinically available collagen membranes may be functionalized by electrospinning poly-DL-lactic acid (PDLLA) polymers to enhance periodontal regeneration.…”