Development of four PCR-based methods to differentiate tilefish species (Branchiostegus japonicus and B. albus)

Kang, Tae Sun

doi:10.1016/j.foodchem.2018.07.138

Cited by 23 publications

(18 citation statements)

References 28 publications

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“…In a real‐time PCR assay, standard curves can be a simple and fast indicator for evaluating amplification efficiency and sensitivity 23 . Therefore, two criteria were used to define an acceptable qPCR assay according to previous studies 24,25 : linear dynamic range and amplification efficiency 26 .…”

Section: Methodsmentioning

confidence: 99%

Development of molecular markers to distinguish between morphologically similar edible plants and poisonous plants using a real‐time PCR assay

Kim

Jang

2020

J Sci Food Agric

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BACKGROUNDAs a result of similar appearances between edible and poisonous plants, 42 patients have ingested poisonous plants from 2013 to 2017 in Korea. We have developed species‐specific primer sets of three of edible and poisonous plants sets (Ligularia fischeri & Caltha palustris, Artemisia annua & Ambrosia artemisiifolia and Hemerocallis fulva & Veratrum maackii) for distinguishing both plants using a real‐time polymerase chain reaction assay.RESULTSThe efficiencies of the developed primer sets ranged from 87.8% to 102.0%. The developed primer sets have significant correlation coefficient values between the Ct values and the log DNA concentration for their target species (r2 > 0.99). The cut‐off lines as the crossing point values of the limit of quantitation of the target species were determined, and all non‐target species were amplified later than the cut‐off cycles. Then, the effectiveness of the developed primer sets was evaluated using commercial food products and digested samples with simulated gastric juice.CONCLUSIONAll of the developed species‐specific primer sets were able to detect target DNA successfully in commercial food products and the digested samples. Therefore, the developed species‐specific primer sets in the present study would be useful tools for distinguishing between poisonous plants and edible plants. © 2020 Society of Chemical Industry

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Section: Methodsmentioning

confidence: 99%

Development of molecular markers to distinguish between morphologically similar edible plants and poisonous plants using a real‐time PCR assay

Kim

Jang

2020

J Sci Food Agric

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“…The time required to detect seafood species in processed foods by using this method is less than that using other PCR-based methods [ 8 , 15 , 39 , 40 ]. Due to the advantages of quick analysis, ultrafast PCR or fast real-time PCR assays have been used to develop methods for the identification of economically motivated food adulteration [ 9 ]. Combining direct DNA extraction and ultrafast PCR allowed us to develop a rapid, cost-effective, and handy monitoring method for on-site analysis.…”

Section: Resultsmentioning

confidence: 99%

“…Polymerase chain reaction (PCR) techniques, which can detect trace amounts of DNA with high specificity and sensitivity, are widely used to authenticate raw materials in food [ 9 , 10 ]. PCR-based techniques, such as DNA barcoding, PCR restriction fragment length polymorphism, forensically informative nucleotide sequencing, and recombinase polymerase amplification, have been developed to detect different species [ 11 , 12 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Rapid On-Site Identification for Three Arcidae Species (Anadara kagoshimensis, Tegillarca granosa, and Anadara broughtonii) Using Ultrafast PCR Combined with Direct DNA Extraction

Lee

Kim

Yang

et al. 2022

Foods

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Granular ark (Tegillarca granosa), broughton’s ribbed ark (Anadara broughtonii), and half-crenate ark (Anadara kagoshimensis) are important fishery resources throughout Asia; granular ark exhibiting a higher economic value due to its rarity. However, due to the similar morphological characteristics of the three species, the less valuable species could be exploited for food fraud. In this study, we developed a rapid on-site identification method based on a microfluidic chip for the detection of the three ark shell species. We designed new species-specific primers, targeting the genes encoding mitochondrial cytochrome b or cytochrome c oxidase I, for the identification of the three ark shells and estimated their specificity against 17 species, which amplified only the target species. The sensitivity of each primer was 0.001 ng. In addition, this method was further improved to develop a direct ultrafast polymerase chain reaction (PCR) for on-site food monitoring, which would allow for completing the entire procedure (from sampling to obtaining the results) within 25 min without DNA extraction. Our direct, ultrafast PCR was successfully applied to differentiate the three species from 29 commercial products. Therefore, this assay could be used as a rapid and cost-effective approach for the on-site identification of ark shells in commercial food products.

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“…Real-time PCR and multiplex PCR methods are the most common detection technologies in meat and meat products, fish and seafood, and other food categories that are known to have a high incidence of adulteration [124,[128][129][130]. There are numerous reports in the literature demonstrating that real-time PCR is a powerful method that can be used as a reliable and sensitive technique for meat identification.…”

Section: Dna-based Techniquesmentioning

confidence: 99%

Fraud in Animal Origin Food Products: Advances in Emerging Spectroscopic Detection Methods over the Past Five Years

Hassoun

Måge

Schmidt

et al. 2020

Foods

111

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Animal origin food products, including fish and seafood, meat and poultry, milk and dairy foods, and other related products play significant roles in human nutrition. However, fraud in this food sector frequently occurs, leading to negative economic impacts on consumers and potential risks to public health and the environment. Therefore, the development of analytical techniques that can rapidly detect fraud and verify the authenticity of such products is of paramount importance. Traditionally, a wide variety of targeted approaches, such as chemical, chromatographic, molecular, and protein-based techniques, among others, have been frequently used to identify animal species, production methods, provenance, and processing of food products. Although these conventional methods are accurate and reliable, they are destructive, time-consuming, and can only be employed at the laboratory scale. On the contrary, alternative methods based mainly on spectroscopy have emerged in recent years as invaluable tools to overcome most of the limitations associated with traditional measurements. The number of scientific studies reporting on various authenticity issues investigated by vibrational spectroscopy, nuclear magnetic resonance, and fluorescence spectroscopy has increased substantially over the past few years, indicating the tremendous potential of these techniques in the fight against food fraud. It is the aim of the present manuscript to review the state-of-the-art research advances since 2015 regarding the use of analytical methods applied to detect fraud in food products of animal origin, with particular attention paid to spectroscopic measurements coupled with chemometric analysis. The opportunities and challenges surrounding the use of spectroscopic techniques and possible future directions will also be discussed.

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Development of four PCR-based methods to differentiate tilefish species (Branchiostegus japonicus and B. albus)

Cited by 23 publications

References 28 publications

Development of molecular markers to distinguish between morphologically similar edible plants and poisonous plants using a real‐time PCR assay

Development of molecular markers to distinguish between morphologically similar edible plants and poisonous plants using a real‐time PCR assay

Rapid On-Site Identification for Three Arcidae Species (Anadara kagoshimensis, Tegillarca granosa, and Anadara broughtonii) Using Ultrafast PCR Combined with Direct DNA Extraction

Fraud in Animal Origin Food Products: Advances in Emerging Spectroscopic Detection Methods over the Past Five Years

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