Development of functionalized multi-walled carbon-nanotube-based alginate hydrogels for enabling biomimetic technologies

Joddar, Binata; Garcia, Eduardo; Casas, Atzimba; Stewart, Calvin M.

doi:10.1038/srep32456

Cited by 107 publications

(103 citation statements)

References 65 publications

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“…Meshes seeded with cells were carefully transferred to unused wells, gently rinsed with PBS, overlaid with 1 mL of 0.25% trypsin-EDTA per well and incubated at 37°C for 10 min. Extracted cells were pelleted by centrifugation, and counted using a hemocytometer throughout the entire culture period after 72 hr of culture following published procedures (63). Similarly cells that adhered to the plastic wells for the respective mesh samples were also trypsin-extracted and estimated after 72 hr of culture.…”

Section: Methodsmentioning

confidence: 99%

Attenuation of the in vitro neurotoxicity of 316L SS by graphene oxide surface coating

Tasnim

Kumar

Joddar

2017

Materials Science and Engineering: C

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A persistent theme in biomaterials research comprises of surface engineering and modification of bare metallic substrates for improved cellular response and biocompatibility. Graphene Oxide (GO), a derivative of graphene, has outstanding chemical and mechanical properties; its large surface to volume ratio, ease of surface modification and processing make GO an attractive coating material. GO-coatings have been extensively studied as biosensors. Further owing to its surface nano-architecture, GO-coated surfaces promote cell adhesion and growth, making it suitable for tissue engineering applications. The need to improve the long-term durability and therapeutic effectiveness of commercially available bare 316L stainless steel (SS) surfaces led us to adopt a polymer-free approach which is cost-effective and scalable. GO was immobilized on to 316L SS utilizing amide linkage, to generate a strongly adherent uniform coating with surface roughness. GO-coated 316 L SS surfaces showed increased hydrophilicity and biocompatibility with SHSY-5Y neuronal cells, which proliferated well and showed decreased reactive oxygen species (ROS) expression. In contrast, cells did not adhere to bare uncoated 316L SS meshes nor maintain viability when cultured in the vicinity of bare meshes. Therefore the combination of the improved surface properties and biocompatibility implies that GO-coating can be utilized to overcome pertinent limitations of bare metallic 316L SS implant surfaces, especially SS neural electrodes. Also, the procedure for making GO-based protective coatings can be applied to numerous other implants where the development of such protective films is necessary.

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Section: Methodsmentioning

confidence: 99%

Attenuation of the in vitro neurotoxicity of 316L SS by graphene oxide surface coating

Tasnim

Kumar

Joddar

2017

Materials Science and Engineering: C

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“…This is manifested by the shift of decomposition temperature of the main polymer blend from 350 • C (Figure 2A-pure hydrogel) to 370 • C. The percent weight loss also decreases with increasing MWCNT content, especially for the highest carbon loading ( Figure 2C). The higher resistance to heat in the presence of the MWCNT reinforced within the polyacrylamide/alginate network can be also related to weak chemical interactions between the MWCNT and the different reaction sites of both polymers [27,29]. For example, the latest study suggested that carboxyl groups present on the MWCNT surface can react with hydroxyl functionalities of alginate, resulting in hydrogen bridging [27].…”

Section: Structure and Thermal Stability: Effect Of Mwcnt Content On mentioning

confidence: 99%

“…The higher resistance to heat in the presence of the MWCNT reinforced within the polyacrylamide/alginate network can be also related to weak chemical interactions between the MWCNT and the different reaction sites of both polymers [27,29]. For example, the latest study suggested that carboxyl groups present on the MWCNT surface can react with hydroxyl functionalities of alginate, resulting in hydrogen bridging [27]. These molecular interactions are believed to contribute to both better thermal stability of the MWCNT/polymer hybrid, and also to improved dispersion of the MWCNT in the solution of monomers and within the hydrogel itself.…”

Section: Structure and Thermal Stability: Effect Of Mwcnt Content On mentioning

confidence: 99%

“…Multi-Walled Carbon Nanotubes (MWCNTs) were chosen as the electron-conducting component of the flexible electrode proposed in this work. MWCNT-alginate hydrogels have been previously explored by Joddar et al [27], with a focus on mechanical properties-notably with overall declining performance above 1 mg/mL of MWCNT (to a tested max of 5 mg/mL). Hong et al [28] integrated single-walled CNTs in poly (dimethyl siloxane) and analyzed their electrical characteristics in various circuits, as well as under differing mechanical strain.…”

Section: Introductionmentioning

confidence: 99%

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Flexible Electrode Based on MWCNT Embedded in a Cross-Linked Acrylamide/Alginate Blend: Conductivity vs. Stretching

Thibodeau

Ignaszak

2020

Polymers

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A polyacrylamide-alginate hydrogel electrolyte, blended with Multi-Walled Carbon Nanotubes (MWCNT) as an electronically conductive fraction, allows for the creation of a flexible, durable, and resilient electrode. The MWCNT content is correlated with mechanical characteristics such as stretch modulus, tensile resistance, and electrical conductivity. The mechanical analysis demonstrates tensile strength that is comparable to similar hydrogels reported in the literature, with increasing strength for MWCNT-embedded hydrogels. The impedance spectroscopy reveals that the total resistance of electrodes decreases with increasing MWCNT content upon elongation and that bending and twisting do not obstruct their conductivity. The MWCNT-inserted hydrogels show mixed ionic and electronic conductivities, both within a range of 1-4 × 10 −2 S cm −1 in a steady state. In addition, the thermal stability of these materials increases with incrementing MWCNT content. This observation agrees with long-term charge-discharge cycling that shows enhanced electrochemical durability of the MWCNT-hydrogel hybrid when compared to pure hydrogel electrolyte. The hydrogel-carbon films demonstrate an increased interfacial double-layer current at a high MWCNT content (giving an area-specific capacitance of~30 mF cm −2 at 2.79 wt.% of MWCNT), which makes them promising candidates as printable and flexible electrodes for lightweight energy storage applications. The maximum content of MWCNT within the polymer electrolyte was estimated at 2.79 wt.%, giving a very elastic polymer electrode with good electrical characteristics.

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“…Similar CNT-dependent trends were reported for other nanocomposite materials. [23] We suggest that at higher CNT concentrations, CNTs were less well dispersed in the matrix [24] and formed bundled aggregates which might have even compromised self-assembly of DFA nanodomains, resulting in a less uniform network. SEM images (Figure 3e) of 6 wt% nanocomposite hydrogel indeed show the presence of CNT aggregates.…”

Section: Mechanical Properties and Toughnessmentioning

confidence: 99%

Carbon Nanotube Reinforced Supramolecular Hydrogels for Bioapplications

Mihajlovic

Dankers

et al. 2018

Macromolecular Bioscience

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Nanocomposite hydrogels based on carbon nanotubes (CNTs) are known to possess remarkable stiffness, electrical, and thermal conductivity. However, they often make use of CNTs as fillers in covalently cross-linked hydrogel networks or involve direct cross-linking between CNTs and polymer chains, limiting processability properties. Herein, nanocomposite hydrogels are developed, in which CNTs are fillers in a physically cross-linked hydrogel. Supramolecular nanocomposites are prepared at various CNT concentrations, ranging from 0.5 to 6 wt%. Incorporation of 3 wt% of CNTs leads to an increase of the material's toughness by over 80%, and it enhances electrical conductivity by 358%, compared to CNT-free hydrogel. Meanwhile, the nanocomposite hydrogels maintain thixotropy and processability, typical of the parent hydrogel. The study also demonstrates that these materials display remarkable cytocompatibility and support cell growth and proliferation, while preserving their functional activities. These supramolecular nanocomposite hydrogels are therefore promising candidates for biomedical applications, in which both toughness and electrical conductivity are important parameters.

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Development of functionalized multi-walled carbon-nanotube-based alginate hydrogels for enabling biomimetic technologies

Cited by 107 publications

References 65 publications

Attenuation of the in vitro neurotoxicity of 316L SS by graphene oxide surface coating

Attenuation of the in vitro neurotoxicity of 316L SS by graphene oxide surface coating

Flexible Electrode Based on MWCNT Embedded in a Cross-Linked Acrylamide/Alginate Blend: Conductivity vs. Stretching

Carbon Nanotube Reinforced Supramolecular Hydrogels for Bioapplications

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