Development of Gastrointestinal Mucosal Barrier. I. The Effect of Age on Intestinal Permeability to Macromolecules

Udall, John N.; Pang, K Y; Fritze, Linda M.S.; Kleinman, Ronald E.; Walker, W. Allan

doi:10.1203/00006450-198103000-00008

Cited by 185 publications

(91 citation statements)

References 3 publications

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“…Consequently, there are no direct quantitations possible from our data on the uptake of intact BSA or OVA. Since the occurrence of foreign food protein in breast milk has been reported both in the experimental animal and in man (23), and since the absorption of antigenically intact BSA is known to be increased in suckling animals (24), changes in the intestinal handling of food antigens in lactating animals could lead to considerable changes in the degree of exposure of immature animals to foreign macromolecular antigens. In that respect, and in the context of current knowledge about the enteromammary immune system (1 1, 25), the observed changes in antigen handling during lactation appear to be part of a system which protects maternal animals and their immature suckling offspring from excessive exposure to food protein antigens.…”

Section: Discussionmentioning

confidence: 99%

Food Proteins and Gut Mucosal Barrier. III. The Influence of Lactation and Prolactin on the in Vitro Binding and Uptake of Bovine Serum Albumin and Ovalbumin in the Rat Jejunum

et al. 1985

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Section: Discussionmentioning

confidence: 99%

Food Proteins and Gut Mucosal Barrier. III. The Influence of Lactation and Prolactin on the in Vitro Binding and Uptake of Bovine Serum Albumin and Ovalbumin in the Rat Jejunum

et al. 1985

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“…Intragastric feeding was performed as previously described. 18 In earlier studies of CM hypersensitivity, we found that oral feeding of milk to 2-to 3-week-old mice induced moderate levels of IgE and immediate skin reactions (itching) after oral feeding of sensitized mice. In some cases the scratching persisted for more than 10 minutes.…”

Section: Mice and Antigen Sensitizationmentioning

confidence: 99%

Murine model of atopic dermatitis associated with food hypersensitivity

Kleiner²,

Huang³

et al. 2001

Journal of Allergy and Clinical Immunology

111

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Background: Atopic dermatitis (AD) is an eczematous skin eruption that generally begins in early infancy and affects up to 12% of the population. The cause of this disorder is not fully understood, although it is frequently the first sign of atopic disease and is characterized by an elevated serum IgE level, eosinophilia, and histologic tissue changes characterized early by spongiosis and a CD4 + T H 2 cellular infiltrate. Hypersensitivity to foods has been implicated as one causative factor in up to 40% of children with moderate-to-severe AD. Objective: The purpose of this study was to establish a murine model of food-induced AD. Methods: Female C3H/HeJ mice were sensitized orally to cow's milk or peanut with a cholera toxin adjuvant and then subjected to low-grade allergen exposure. Histologic examination of skin lesions, allergen-specific serum Ig levels, and allergen-induced T-cell proliferation and cytokine production were examined. Atopic dermatitis (AD) is a form of eczema that generally begins in early infancy and is characterized by extreme pruritus, chronically relapsing course, and distinctive distribution. AD is often the first sign seen in a child who is prone to atopic disease, with 50% of all AD developing in the first year of life and 80% developing by 5 years of age. 1 In chronic skin lesions the epidermis has moderate-to-marked hyperplasia, with elongation of the rete ridges and prominent hyperkeratosis. Spongiosis is variable, and the numbers of mast cells and Langerhans cells are significantly increased. 2,3 Eosinophils are sparse, although eosinophil products (major basic protein) are prominent. 4 Although the pathogenic role of allergy in AD has been debated for over a century, clinical studies over the past 2 decades have supported the pathogenic role of food allergy in a subset of patients with AD. 5-7 A recent study found that approximately 40% of children with moderate-to-severe AD attending a university dermatology clinic had food hypersensitivity. 7 Other studies delineating the role of IgE-bearing antigen-presenting cells in establishing T H 2 lymphocytic responses 8 and demonstrating the predominant T H 2-lymphocytic infiltrate in acute eczematous lesions 9 have provided further support for the pathogenic role of allergy in AD.Animal models provide powerful tools for dissecting pathogenic mechanisms responsible for various disorders. A number of murine models of asthma have been developed in the past several years that have contributed greatly to our understanding of the immunopathogenesis of allergen-induced asthma. 10-12 Models of antigenassociated AD have been reported in cats and dogs, but because of difficulties with consistent induction of ADlike eruptions and high expense, they have not been widely studied. 13,14 A mouse strain, NC/Nga, has been reported that spontaneously has an AD-like skin lesion and IgE hyperproduction, presumably triggered by environmental factors. 15 More recently, a murine model of AD was described in which BALB/c mice were sensitized to ovalbumin (OVA...

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“…It is subject to modulations by multiple factors such as weaning and hormonal control (16). Studies in different experimental animal species have shown that intestinal maturation affects gut mucosal barrier functions such as macromolecular antigen handling in the everted gut sac model (34) and uptake of orally administered protein in the whole animal model (13,32,33). The increased absorption of intact BSA during early postnatal age has been shown directly in rabbits, and it was shown to decrease significantly after 1 wk of age (33).…”

mentioning

confidence: 99%

“…Studies in different experimental animal species have shown that intestinal maturation affects gut mucosal barrier functions such as macromolecular antigen handling in the everted gut sac model (34) and uptake of orally administered protein in the whole animal model (13,32,33). The increased absorption of intact BSA during early postnatal age has been shown directly in rabbits, and it was shown to decrease significantly after 1 wk of age (33). In the same postnatal period, the intestinal surface is undergoing structural and biochemical changes such as a decrease in fluidity of MVM and changes in binding capacity and kinetics of calcium, cholera toxin, and lectins (5,19,23).…”

mentioning

confidence: 99%

Food Proteins and Gut Mucosal Barrier. II. Differential Interaction of Cow's Milk Proteins with the Mucous Coat and the Surface Membrane of Adult and Immature Rat Jejunum

1984

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ABSTRACT. Two in vitro intestinal models were used to investigate postnatal maturational changes of the gut barrier functions. Microvillus membrane (MVM) preparations were studied for surface binding, and everted gut sacs were studied for mucous coat binding, breakdown and uptake of radioiodinated bovine serum albumin (BSA), and @-lactoglobulin (BLG). Surface binding of these proteins to MVM was weak and nonspecific. There was more binding of both proteins to immature MVM (BSA: newborns, 2.74 + 0.52%, adults, 1.08 f 0.17%,p < 0.001; @-LG: newborns, 6.30 2 0.54%; adults, 2.05 f 0.07, p < 0.001). In contrast to MVM binding characteristics, mu-

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Development of Gastrointestinal Mucosal Barrier. I. The Effect of Age on Intestinal Permeability to Macromolecules

Cited by 185 publications

References 3 publications

Food Proteins and Gut Mucosal Barrier. III. The Influence of Lactation and Prolactin on the in Vitro Binding and Uptake of Bovine Serum Albumin and Ovalbumin in the Rat Jejunum

Food Proteins and Gut Mucosal Barrier. III. The Influence of Lactation and Prolactin on the in Vitro Binding and Uptake of Bovine Serum Albumin and Ovalbumin in the Rat Jejunum

Murine model of atopic dermatitis associated with food hypersensitivity

Food Proteins and Gut Mucosal Barrier. II. Differential Interaction of Cow's Milk Proteins with the Mucous Coat and the Surface Membrane of Adult and Immature Rat Jejunum

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