Development of gelatin/ascorbic acid cryogels for potential use in corneal stromal tissue engineering

Luo, Lan; Lai, Jui‐Yang; Chou, Shih-Feng; Hsueh, Yi‐Jen; Hui‐Kang, David

doi:10.1016/j.actbio.2017.11.018

Cited by 61 publications

(48 citation statements)

References 73 publications

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“…Various bioengineering approaches have been attempted to fabricate corneal equivalence based on natural (e.g., collagen 1,4,5 , gelatin 6 , chitosan 7 , silk 8 , etc) or synthetic (e.g., poly (ethylene glycol) (PEG) 9 , poly (ε-caprolactone) (PCL) 10 , poly(lactic-co-glycolic acid) (PLGA) 1,11 , poly-hydroxyethylmethacrylate (PHEMA) 12 , etc) materials or the combination of natural and synthetic materials 1,4,13 by using the techniques of casting 14 , hydrogel 15 , 3D printing 16 , electrospinning 17,18 , and the combination of two or more of these processes 17 . Although these therapies and constructs have demonstrated acceptable mechanical properties and optical transmittance and can support corneal cells adhesion, migration, proliferation, and differentiation well, they fail to mimic the natural microenvironment of the native complex corneal tissue, and the most complicated part among the corneal tissue is the stroma.…”

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confidence: 99%

Fiber reinforced GelMA hydrogel to induce the regeneration of corneal stroma

Kong

Chen²,

Li³

et al. 2020

Nat Commun

228

190

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Regeneration of corneal stroma has always been a challenge due to its sophisticated structure and keratocyte-fibroblast transformation. In this study, we fabricate grid poly (ε-caprolactone)-poly (ethylene glycol) microfibrous scaffold and infuse the scaffold with gelatin methacrylate (GelMA) hydrogel to obtain a 3 D fiber hydrogel construct; the fiber spacing is adjusted to fabricate optimal construct that simulates the stromal structure with properties most similar to the native cornea. The topological structure (3 D fiber hydrogel, 3 D GelMA hydrogel, and 2 D culture dish) and chemical factors (serum, ascorbic acid, insulin, and β-FGF) are examined to study their effects on the differentiation of limbal stromal stem cells to keratocytes or fibroblasts and the phenotype maintenance, in vitro and in vivo tissue regeneration. The results demonstrate that fiber hydrogel and serum-free media synergize to provide an optimal environment for the maintenance of keratocyte phenotype and the regeneration of damaged corneal stroma.

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confidence: 99%

Fiber reinforced GelMA hydrogel to induce the regeneration of corneal stroma

Kong

Chen²,

Li³

et al. 2020

Nat Commun

228

190

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“…Gelatin is a soluble substance easily obtained from various animals, including cows, pigs, and fish (29,32). This biomaterial is widely used in tissue engineering, such as endothelial cell sheet delivery and stromal tissue grafting, and is clinically proven to be safe (29,30,38,39). Collagen is a protein extracted from the skin and bones of various mammals and is categorized into types (I, II, III, V, and VI) according to type of protein.…”

Section: Discussionmentioning

confidence: 99%

In vivo evaluation of scaffolds compatible for colonoid engraftments onto injured mouse colon epithelium

et al. 2019

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Colon organoids (colonoids) are known to be similar to colon tissue in structure and function, which makes them useful in the treatment of intestinal de‐epithelialized disease. Matrigel, which is used as a transplantation scaffold for colonoids, cannot be used in clinical applications because of its undefined composition and tumorigenicity. This study identifies clinically available scaffolds that are effective for colonoid transplantation in damaged intestinal mucosa. The colon crypt was isolated and cultured from C57BL/6‐Tg[CAG enhanced green fluorescent protein (EGFP)1310sb/LeySopJ mice into EGFP + colonoids and subsequently transplanted into the EDTA colitis mouse model using gelatin, collagen, or fibrin glue scaffolds. To identify scaffolds suitable for colonoid engraftment in injured colon mucosa, the success rates of transplantation and secondary EGFP colonoid formation were measured, and the scaffolds' mediated toxicity in vitro and in vivo was observed in recipient mice. When colonoids were transplanted with gelatin, collagen, and fibrin glue into the EDTA colitis mouse model, all groups were found to be successfully engrafted. Fibrin glue, especially, showed significant increase in the engrafted area compared with Matrigel after 4 wk. The scaffolds used in the study did not induce colonic toxicity after transplantation into the recipients' colons and were thus deemed safe when locally administrated. This study suggests new methods for and provides evidence of the safety and utility of the clinical application of colonoid‐based therapeutics. Furthermore, the methods introduced in this study will be helpful in developing cell treatment using the esophagus or a stomach organoid for various digestive‐system diseases.—Jee, J., Jeong, S. Y., Kim, H. K., Choi, S. Y., Jeong, S., Lee, J., Ko, J. S., Kim, M. S., Kwon, M.‐S., Yoo, J. In vivo evaluation of scaffolds compatible for colonoid engraftments onto injured mouse colon epithelium. FASEB J. 33, 10116–10125 (2019). http://www.fasebj.org

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“…Gelatin, a single-stranded protein extracted from collagen, is widely used for various biomedical applications due to its excellent biological characteristics and tunable physical properties upon chemical functionalization. [1][2][3][4] To date, animal-derived gelatin has frequently been used to produce cell-interactive scaffolds for the regeneration of adipose tissue. [5][6][7] This type of tissue is often applied to restore volume loss in patients suffering from congenital defects, trauma or surgical deformities.…”

Section: Introductionmentioning

confidence: 99%