Development of genistein-loaded gold nanoparticles and their antitumor potential against prostate cancer cell lines

Vodnik, Vesna; Mojić, Marija; Stamenović, Una; Otoničar, Mojca; Ajdžanović, Vladimir; Maksimović‐Ivanić, Danijela; Mijatović, Sanja; Marković, Mirjana; Barudžija, Tanja; Filipović, Branka; Milošević, Verica; Šošić‐Jurjević, Branka

doi:10.1016/j.msec.2021.112078

Cited by 42 publications

(26 citation statements)

References 40 publications

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“…Our previous studies showed that genistein interacts electrostatically with gold nanoparticles [5] which turned out to be interesting carriers for active substances. These studies were continued by Vodnik et al [6], however the authors did not perform a purity study of conjugates in relation to the free GE and stability of new conjugates as well. It is known that electrostatic interactions are not sufficiently stable at extreme pH and in the presence of salt.…”

Section: Introductionmentioning

confidence: 99%

Anti-Cancer and Electrochemical Properties of Thiogenistein—New Biologically Active Compound

Stolarczyk

Strzempek

Łaszcz

et al. 2021

IJMS

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Pharmacological and nutraceutical effects of isoflavones, which include genistein (GE), are attributed to their antioxidant activity protecting cells against carcinogenesis. The knowledge of the oxidation mechanisms of an active substance is crucial to determine its pharmacological properties. The aim of the present work was to explain complex oxidation processes that have been simulated during voltammetric experiments for our new thiolated genistein analog (TGE) that formed the self-assembled monolayer (SAM) on the gold electrode. The thiol linker assured a strong interaction of sulfur nucleophiles with the gold surface. The research comprised of the study of TGE oxidative properties, IR-ATR, and MALDI-TOF measurements of SAM before and after electrochemical oxidation. TGE has been shown to be electrochemically active. It undergoes one irreversible oxidation reaction and one quasi-reversible oxidation reaction in PBS buffer at pH 7.4.. The oxidation of TGE results in electroactive products composed likely from TGE conjugates (e.g., trimers) as part of polymer. The electroactive centers of TGE and its oxidation mechanism were discussed using IR supported by quantum chemical and molecular mechanics calculations. Preliminary in-vitro studies indicate that TGE exhibits higher cytotoxic activity towards DU145 human prostate cancer cells and is safer for normal prostate epithelial cells (PNT2) than genistein itself.

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Section: Introductionmentioning

confidence: 99%

Anti-Cancer and Electrochemical Properties of Thiogenistein—New Biologically Active Compound

Stolarczyk

Strzempek

Łaszcz

et al. 2021

IJMS

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“…Gold NP-loaded genistein was assessed for its anti-cancer role in prostate cancer cells. Genistein selectivity for prostate cancer and bioavailability was significantly improved by conjugating it with gold NP [ 91 ]. However, the chemotherapeutic applications of genistein are influenced by its before mentioned unwanted properties.…”

Section: Introductionmentioning

confidence: 99%

Genistein as a regulator of signaling pathways and microRNAs in different types of cancers

et al. 2021

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Cancers are complex diseases orchestrated by a plethora of extrinsic and intrinsic factors. Research spanning over several decades has provided better understanding of complex molecular interactions responsible for the multifaceted nature of cancer. Recent advances in the field of next generation sequencing and functional genomics have brought us closer towards unravelling the complexities of tumor microenvironment (tumor heterogeneity) and deregulated signaling cascades responsible for proliferation and survival of tumor cells. Phytochemicals have begun to emerge as potent beneficial substances aimed to target deregulated signaling pathways. Isoflavonoid genistein is an essential phytochemical involved in regulation of key biological processes including those in different types of cancer. Emerging preclinical evidence have shown its anti-cancer, anti-inflammatory and anti-oxidant properties. Testing of this substance is in various phases of clinical trials. Comprehensive preclinical and clinical trials data is providing insight on genistein as a modulator of various signaling pathways both at transcription and translation levels. In this review we have explained the mechanistic regulation of several key cellular pathways by genistein. We have also addressed in detail various microRNAs regulated by genistein in different types of cancer. Moreover, application of nano-formulations to increase the efficiency of genistein is also discussed. Understanding the pleiotropic potential of genistein to regulate key cellular pathways and development of efficient drug delivery system will bring us a step towards designing better chemotherapeutics.

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“… 50 PEG at metal surface of BNPs experiences probably increased in the oscillator strength and orientation of molecule. 51 It is demonstrated that controlled aggregation can produce a significant increase in the SERS response from silver nanoparticles. Dimer nanoparticles of BNPs and also presence of silver play a role in hot spot formation.…”

Section: Resultsmentioning

confidence: 99%

“…In a similar study, pharmaceutical excipient of the Raman spectrum of the AgNPs colloid vibrational modes characteristic to SDS showed 20cm −1 Raman shift. 50 PEG at metal surface of BNPs experiences probably increased in the oscillator strength and orientation of molecule. 51 It is demonstrated that controlled aggregation can produce a significant increase in the SERS response from silver nanoparticles.…”

Section: Raman Spectroscopy Of Peg 600 and Bnpsmentioning

confidence: 98%

One Pot Synthesis of PEGylated Bimetallic Gold–Silver Nanoparticles for Imaging and Radiosensitization of Oral Cancers

Ahmed¹,

Baijal

Somashekar³

et al. 2021

IJN

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Background:Radiotherapy is an important treatment modality for many types of head and neck squamous cell carcinomas. Nanomaterials comprised of high atomic number (Z) elements are novel radiosensitizers enhance radiation injury by production of free radicals and subsequent DNA damage. Gold nanoparticles are upcoming as promising radiosensitizers due to their high (Z) biocompatibility, and ease for surface engineering. Bimetallic nanoparticles have shown enhanced anticancer activity compared to monometallic nanoparticles. Materials and Methods: PEG-coated Au-Ag alloy nanoparticles (BNPs) were synthesized using facile one pot synthesis techniques. Size of ~50±5nm measured by dynamic light scattering. Morphology, structural composition and elemental mapping were analyzed by electron microscopy and SAXS (small-angle X-ray scattering). The radiosensitization effects on KB oral cancer cells were evaluated by irradiation with 6MV X-rays on linear accelerator. Nuclear damage was imaged using confocal microscopy staining cells with Hoechst stain. Computed tomography (CT) contrast enhancement of BNPs was compared to that of the clinically used agent, Omnipaque. Results: BNPs were synthesized using PEG 600 as reducing and stabilizing agent. The surface charge of well dispersed colloidal BNPs solution was −5mV. Electron microscopy reveals spherical morphology. HAADF-STEM and elemental mapping studies showed that the constituent metals were Au and Ag intermixed nanoalloy. Hydrodynamic diameter was ~50±5nm due to PEG layer and water molecules absorption. SAXS measurement confirmed BNPs size around 35nm. Raman shift of around 20 cm −1 was observed when BNPs were coated with PEG. 1 H NMR showed extended involvement of − OH in synthesis. BNPs efficiently enter cytoplasm of KB cells and demonstrated potent in vitro radiosensitization with enhancement ratio ~1.5-1.7. Imaging Hoechst-stained nuclei demonstrated apoptosis in a dose-dependent manner. BNPs exhibit better CT contrast enhancement ability compared to Omnipaque. Conclusion: This bimetallic intermix nanoparticles could serve a dual function as radiosensitizer and CT contrast agent against oral cancers, and by extension possibly other cancers as well.

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Development of genistein-loaded gold nanoparticles and their antitumor potential against prostate cancer cell lines

Cited by 42 publications

References 40 publications

Anti-Cancer and Electrochemical Properties of Thiogenistein—New Biologically Active Compound

Anti-Cancer and Electrochemical Properties of Thiogenistein—New Biologically Active Compound

Genistein as a regulator of signaling pathways and microRNAs in different types of cancers

One Pot Synthesis of PEGylated Bimetallic Gold–Silver Nanoparticles for Imaging and Radiosensitization of Oral Cancers

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