Development of Glucose Intolerance in Wistar Rats Fed Low and Moderate Fat Diets Differing in Fatty Acid Profile

Abstract

A moderate increase in dietary fat (20%), within the recommended nutritional range, and an unfavourable omega6:omega3 ratio resulted in glucose intolerance in this Wistar rat model, which was exacerbated with a further increase in dietary fat (30%).

Importance of Dietary Fatty Acid Profile and Experimental Conditions in the Obese Insulin-Resistant Rodent Model of Metabolic Syndrome

“…This also supports earlier findings that a circadian rhythm for eicosanoid production exists, with low levels during the inactive phase and high levels present during the active phase [92]. In light of the fact the plasma FFA profile represents the dietary FFA profile [77], it is likely that the levels omega-6 HUFAs will be increased when a diet high in omega-6 fatty acids is followed. As arachidonic acid, via NFkB and eicosanoid conversions products, causes a suppression of adiponectin, this would exaggerate and amplify the pro-diabetic, insulin resistance and hyperglycemic propensity of the hormonal profile of the inactive phase.…”

“…A rodent high fat diet high in SFA content, in the presence of a low omega-6:omega-3 ratio would not give the same results as a diet similar in total fat and SFA content, but with a high omega-6:omega-3 ratio. Even with low to moderate fat percentages (20-30%) in the presence of the recommended SFA content (10%), high omega-6:omega-3 ratios can lead to glucose intolerance in rodents indicated by decreased insulin response to intravenous glucose tolerance tests [77].…”

Aspalathin-Enriched Green Rooibos Extract Reduces Hepatic Insulin Resistance by Modulating PI3K/AKT and AMPK Pathways

“…Wistar rats ( n = 60), 12 rats per group were fed a high-fat, high-sugar diet (40% fat as energy of which 18.3% were saturated fats) and 44% carbohydrates (2.06 Kcal/g). In addition, 30% sucrose and fructose (1:1 w / w ) was added in drinking water ad libitum for 12 weeks to induce obesity and insulin resistance (OBIR), according to an already published model [52]. After 12 weeks on the high-fat diet, the rats were maintained on the diet with daily administration of GRE at different doses (32, 97, and 195 mg/kg) for a further 12 weeks.…”

Assessment of the Antidiabetic Potential of an Aqueous Extract of Honeybush (Cyclopia intermedia) in Streptozotocin and Obese Insulin Resistant Wistar Rats

“…The high fat diet in combination with sucrose induces insulin resistance and obesity with slightly elevated fasting glucose concentrations (Hallfrisch et al, 1981;Krygsman et al, 2010). After 9 wks on the high fat and sucrose diet, blood was collected for baseline glucose and insulin determination.…”