Development of Growth Medium for Centella Asiatica Cell Culture Via Response Surface Methodology

Omar, Rozita; Abdullah, Monis Abdulmanan; Hasan, M. A.; Marziah, M.

doi:10.3844/ajassp.2004.215.219

Cited by 15 publications

(7 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In C. asiatica cell suspension cultures, RSM applied for the optimization of sucrose, IAA, and 6benzylaminopurine (BAP) and results revealed sucrose as a prime factor for cell growth. 30 Several studies found the significance of applying RSM. 22 RSM was used for the optimization of basal media components (sucrose, nitrogen, and phosphorus) for maximum production of β-carotene from D. carota cells.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Isoflavone Augmentation in Soybean Cell Cultures Is Optimized Using Response Surface Methodology

Devi

Giridhar

2014

J. Agric. Food Chem.

View full text Add to dashboard Cite

Glycine max contains potential therapeutic isoflavones, and its productivity in plants is considerably influenced worldwide by several biotic and abiotic factors. Optimization of soybean cell suspension cultures (Indian variety, JS 335) to maximize the cell growth and isoflavone production in the present study was performed using response surface methodology (RSM) with three independent variables of plant growth regulators, 2,4-dichlorophenoxyacetic acid (2,4-D), 1-naphthalene acetic acid (α-NAA), and kinetin (Kn). The maximum biomass achieved was 70.62 g/L dry weight (dw) using the optimized medium of 2.10 mg/L 2,4-D, 5.52 mg/L α-NAA, and 0.35 mg/L Kn supplemented in the Murashige and Skoog (MS) basal medium. The total isoflavone content of 38.59 mg/g of dw was obtained in the medium with optimized conditions of 1.33 mg/L 2,4-D, 1.76 mg/L α-NAA, and 0.15 mg/L Kn. In comparison to field-grown soybean seeds, the cell suspension cultures profoundly augmented isoflavone concentrations. The optimized conditions for both biomass and total isoflavone content were evaluated by superimposing the contour plots. The results suggested that the optimized medium of cell suspension cultures possibly be used for scale-up studies in bioreactors to offer a high content of bioactive isoflavones.

show abstract

Section: ■ Results and Discussionmentioning

confidence: 99%

“…max suspension cultures was achieved. In the same way, this RSM technique has been applied successfully nowadays in many biotechnological and biochemical studies, such as in vitro growth characteristics of Centella asiatica, β-carotene in D. carota, lycopene from suspension cultures of L.…”

Section: Resultsmentioning

confidence: 99%

Isoflavone Augmentation in Soybean Cell Cultures Is Optimized Using Response Surface Methodology

Devi

Giridhar

2014

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…Studies by George et al (2000) indicated that higher sucrose concentration with low NAA and higher BAP resulted in maximum shoot length. Similar studies have also been done where RSM-CCD was used for the development of cell culture medium of Centella asiatica where the effects of sucrose, IAA and BAP were studied where maximum dry cell weight was obtained at 6% sucrose and 1 mg.L -1 of BAP and IAA (Omar et al, 2004). Naveenchandra et al (2011) also observed that maximum sucrose concentration was one of the contributing factors for high shoot initiation.…”

Section: Regression and Statistical Analysismentioning

confidence: 94%

Optimization of Shoot Initiation Using Response Surface Methodology and Callus Media Standardization for Micropropagation of Centella Asiatica

Gururajan¹,

Hegde²,

Udupa³

et al. 2021

J microb biotech food sci

View full text Add to dashboard Cite

Centella asiatica, a medicinal herb consumed as a food supplement and used in traditional and folklore medicine is being overexploited for its therapeutic properties. Lack of adequate cultivation and replacement practices could engender the depletion of the wild variety. Application of plant tissue culture has helped in the regeneration and preservation of several plants in-vitro. The present study reports media standardization for micropropagation and callus induction for the conservation of Centella asiatica using field-grown plants. In media standardization for callus induction, appreciable results were obtained where full strength MS (Murashige and Skoog) media was supplemented with 2 mg.L-1 IAA (Indole acetic acid) and a combination of 0.5 mg.L-1 Kn (Kinetin) with 1 mg.L-1 IAA while MS media supplemented with a combination of 0.5 mg.L-1 Kn and 1.5 mg.L-1 IAA was used for callus proliferation. Combination of BAP (6- Benzyl amino purine) and IAA from preliminary experimentation on micropropagation showed high shooting frequency and were further optimized with parameters such as sucrose and incubation period using Response surface methodology (RSM) to study their individual and interactive effects. A maximum shoot height of 3.8 cm was obtained from the optimized values after 13 days with both BAP and IAA at 0.5 mg.L-1 and sucrose at 6% with the R2 value at 0.73.

show abstract

“…This method has been applied to optimize biochemical and physical process in many researches (Cansee et al, 2008;Omar et al, 2004;Zhu et al, 2010;Xu et al, 2008;Narkprasom et al, 2013) because, this excellent design spends minimal experiment to obtain the best outcome.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Total Phenolic from <i>Cleistocalyx nervosum</i> by Microwave-Assisted Extraction

Narkprasom

Upara

2015

American Journal of Engineering and Applied Sciences

View full text Add to dashboard Cite

Abstract:The objective of this research was to extract phenolic compounds from Makiang (Cleistocalyx nervosum) seeds waste obtained from the elaboration of makiang beverages using the microwave assisted extraction method. The optimal conditions of microwave assisted extraction of total phenolics from makiang seeds were determined using response surface method. The variables of microwave power, extraction time and ethanol proportion on effect of total phenolic were designed the experiment by Box Behnken design. The estimation of the mathematical model indicated that the second-order polynomial model was appropriate to determine optimal conditions of microwave assisted extraction of total phenolics from makiang seeds. The highest yield of total phenolic from makiang seed and antioxidant activity were obtained when the extraction process was set at a microwave power, 450 W, an extraction time of 213 second and an ethanol proportion of 51%(v/v). Under these optimal conditions, the predicted and experimental values of total phenolic from makiang seeds were the 75.659 mgGAE/gDW and 75.132±0.576 mgGAE/gDW, respectively. The research showed that total phenolics from makiang seeds by microwave assisted extraction have the high efficiency in terms of high yield and antioxidant activity within short time extraction which can apply to use in cosmetic product, health food and pharmaceutical industry.

show abstract

Development of Growth Medium for Centella Asiatica Cell Culture Via Response Surface Methodology

Cited by 15 publications

References 12 publications

Isoflavone Augmentation in Soybean Cell Cultures Is Optimized Using Response Surface Methodology

Isoflavone Augmentation in Soybean Cell Cultures Is Optimized Using Response Surface Methodology

Optimization of Shoot Initiation Using Response Surface Methodology and Callus Media Standardization for Micropropagation of Centella Asiatica

Optimization of Total Phenolic from <i>Cleistocalyx nervosum</i> by Microwave-Assisted Extraction

Contact Info

Product

Resources

About