Strut-and-tie modelling (STM) is an effective and widely used method to design disturbed regions (D-regions) of reinforced concrete structures. Among the various steps of STM, finding a suitable truss-analogy model is the most challenging part. Even for experienced engineers it is difficult to find representative models for complex D-regions, and this task is even harder for three-dimensional (3D) Dregions. To date, only a few 3D STM models have been proposed by researchers for several complex D-regions, which leaves practitioners with little guidance.In order to solve this problem, a method is proposed to automatically generate 3D optimization-based STM (3D-OPT-STM) models. The generation method comprises a compliance-based topology optimization process that generates optimized structural forms by maximizing stiffness, a topology extraction method, and a shape optimization method. In this study, three 3D-OPT-STM models are generated for typical 3D D-regions, and their performances are compared to manually created STM models. The generated 3D-OPT-STM models result in more economical designs. Moreover, geometrical and loading parameter studies demonstrate the applicability and robustness of the proposed method.This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.