2010
DOI: 10.1371/journal.pone.0010150
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Development of Hepatitis C Virus Genotyping by Real-Time PCR Based on the NS5B Region

Abstract: BackgroundHepatitis C virus (HCV) genotyping is the most significant predictor of the response to antiviral therapy. The aim of this study was to develop and evaluate a novel real-time PCR method for HCV genotyping based on the NS5B region.Methodology/Principal FindingsTwo triplex reaction sets were designed, one to detect genotypes 1a, 1b and 3a; and another to detect genotypes 2a, 2b, and 2c. This approach had an overall sensitivity of 97.0%, detecting 295 of the 304 tested samples. All samples genotyped by … Show more

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Cited by 20 publications
(28 citation statements)
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“…This is in contrast to the study by Nakatani et al [35] who reported the limit to be 125 -500 IU/ mL. The current genotyping method presented an overall detection rate of 93 out of 98 (94.89%) patients included in this study.…”
Section: Discussioncontrasting
confidence: 89%
“…This is in contrast to the study by Nakatani et al [35] who reported the limit to be 125 -500 IU/ mL. The current genotyping method presented an overall detection rate of 93 out of 98 (94.89%) patients included in this study.…”
Section: Discussioncontrasting
confidence: 89%
“…We also examined the method for identifying HCV genotypes by real-time PCR-based method in the HCV NS5B region [12] and analyzed the specific probe region for HCV subgenotypes 1a and 1b using ultra-deep sequencing.…”
Section: Introductionmentioning
confidence: 99%
“…However, like in other assay systems it is not possible to detect all subtypes by using 5'NCR [12]. Attempts have been made to include the conserved part of other subgenomic regions also both in PCR-RFLP as well as Real Time PCR [40]. Present study was one more attempt to develop assays for determining HCV sub-types using HCV core region for amplification followed by RFLP with different sets of restriction enzymes.…”
Section: Discussionmentioning
confidence: 99%