Development of hepatorenal syndrome in bile duct ligated rats

Pereira, Regina Maria; Santos, Robson A.S.; Oliveira, Eduardo A.; Leite, Virgínia Hora Rios; Dias, Filipi L C; Rezende, Alysson S; Costa, Luciane Rezende; Barcelos, Lucíola da Silva; Teixeira, Mauro M.; Silva, Ana Cristina Simões e

doi:10.3748/wjg.14.4505

Cited by 36 publications

(41 citation statements)

References 28 publications

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“…This complication has been observed in the presence of hyperbilirubinemia and increased bile acids 10,20 . Functional changes of the kidney are linked to etiological agents or hemodynamic changes, characteristic of chronic liver disease, in which there is peripheral vasodilation, renal vasoconstriction and activation of the renin-angiotensin system [21][22][23][24] . There is also increased oxidative stress and increased production of free radicals 25,26 , as well as nephrotoxicity caused directly and indirectly by bile acids and bilirubin, especially in situations of renal ischemia 10,[27][28][29][30] .…”

Section: Results Results Results Resultsmentioning

confidence: 99%

Influence of distal ileum exclusion on hepatic and renal functions in presence of extrahepatic cholestasis

Costa

Petroianu²,

Júnior³

2014

Rev. Col. Bras. Cir.

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OBJECTIVE: To verify whether the ileal exclusion interferes with liver and kidney functional changes secondary to extrahepatic cholestasis.METHODS: We studied 24 rats, divided into three groups with eight individuals each: Group 1 (control), Group 2 (ligation of the hepatic duct combined with internal biliary drainage), and Group 3 (bile duct ligation combined with internal biliary drainage and exclusion of the terminal ileum). Animals in Group 1 (control) underwent sham laparotomy. The animals of groups 2 and 3 underwent ligation and section of the hepatic duct and were kept in cholestasis for four weeks. Next, they underwent an internal biliary bypass. In Group 3, besides the biliary-enteric bypass, we associated the exclusion of the last ten centimeters of the terminal ileum and carried out an ileocolic anastomosis. After four weeks of monitoring, blood was collected from all animals of the three groups for liver and kidney biochemical evaluation (albumin, ALT, AST, direct and indirect bilirubin, alkaline phosphatase, cGT, creatinine and urea).RESULTS: there were increased values of ALT, AST, direct bilirubin, cGT, creatinine and urea in rats from Group 3 (p < 0.05).CONCLUSION: ileal exclusion worsened liver and kidney functions in the murine model of extrahepatic cholestasis, being disadvantageous as therapeutic procedure for cholestatic disorders.

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Section: Results Results Results Resultsmentioning

confidence: 99%

Influence of distal ileum exclusion on hepatic and renal functions in presence of extrahepatic cholestasis

Costa

Petroianu²,

Júnior³

2014

Rev. Col. Bras. Cir.

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“…BDL rats after four weeks of biliary obstruction present an initial disturbance in renal function associated with ascites. Two weeks later, rats with obstructive cholestasis clearly developed hepatorenal syndrome with ascites [156]. In these chronic phases of macrosurgical obstructive cholestasis, high rates of bacterial translocation, with endotoxemia and consecutive systemic inflammatory response, also exist [157,158].…”

Section: Obstructive Cholestasis In the Rat: A Model Of Decompensatedmentioning

confidence: 99%

Portal hypertension-related inflammatory phenotypes: From a vitelline and amniotic point of view

Aller¹,

Arias²,

Prieto³

et al. 2012

ABB

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Prehepatic portal hypertension induces a splanchnic low-grade inflammatory response that could switch to high-grade inflammation with the development of severe and life-threatening complications when associated with chronic liver disease. The extraembryonic origin of the portal system maybe determines the regression to an extraembryonic phenotype, i.e., vitellogenic and amniotic, during the evolution of both types of portal hypertension. Thus, prehepatic portal hypertension, or compensated hypertension by portal vein ligation in the rat, is associated with molecular mechanisms related to vitellogenesis, where hepatic steatosis and splanchnic angiogenesis stand out. In turn, extrahepatic cholestasis in the rat induces intrahepatic portal hypertension, or decompensated hypertension, with ascites and hepatorenal syndrome. The splanchnic interstitium, the mesenteric lymphatic system, and the peritoneal mesothelium seem to create an inflammatory pathway that could have a key pathophysiological relevance in the production of ascites. The hypothetical comparison between the ascitic and the amniotic fluid also allows for translational investtigation. The induced regression of the splanchnic system to extraembryonic functions by portal hypertension highlights the great relevance of the extraembryonic structures even during post-natal life.

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“…Pereira et al 31 showed that rats with 2 wk of BDL increased free water clearance reduced urinary osmolality and serum creatinine in comparison to the sham group. In contrast, rats at 6 wk of BDL showed features of HRS, including significant increase in serum creatinine and reductions in creatinine clearance, water excretion and urinary sodium concentration.…”

Section: Renal Morphologymentioning

confidence: 99%

Effect of sirolimus on renal injury induced by bile duct ligation in rats

et al. 2010

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Purpose:To evaluate the effects of sirolimus (SRL) on renal injury in rats with bile duct ligation. Methods: A total of 21 male Sprague-Dawley rats weighing 220-260g were used. Group 1 (Sham-control, n=7) rats were undergone laparotomy alone and bile duct was just dissected from the surrounding tissue. Group 2 rats (BDL/Untreated, n=7) were subjected to bile duct ligation and no drug was applied. Group 3 rats (BDL/SRL, n =7) received a daily dose of sirolimus (0.5 mg·day -1 ×kg -1 dissolved 1 ml in saline) by orogastric tube for 14 days after BDL. At the end of the two-week period, biochemical and histological evaluation were processed. Results: AST, ALT, AP and TB levels values were decreased in group 3 when compared to group 2. There was no significant difference in serum levels of BUN and creatinine among all the experimental groups. Histological evaluation of the liver of BDL/Untreated group rats demonstrated marked portal fibrosis and signs of major bile duct obstruction with prominent portal and lobular inflammation. In BDL/SRL group, moderate damage was seen. Tubular injury scores were higher in the BDL subgroups; however, group 3 rats showed considerably fewer lesions in the tubules and interstitium compared to the group 2 rats. In group 2 animals, in the epithelial cells of proximal tubules presented vacuoles and hydropic changes, atrophy and inflammatory cell infiltrate in the medullar interstitium. Conclusions: Sirolimus decreased tubulointerstitial lesions in kidney induced by bile duct ligation in rats. The improve effects of sirolimus on renal morphology can be due to improved liver function or due to direct action on the kidney. Key words: Sirolimus. Common Bile Duct. Ligation. Liver. Kidney. Rats. RESUMO Objetivo:Investigar os efeitos do sirolimo (SRL) na lesão renal induzida pela ligadura do ducto biliar em ratos. Métodos: Foram utilizados 21 ratos Sprague-Dawley pesando entre 220-260 g. Grupo 1 (Sham-controle, n=7) submetidos a laparotomia e o ducto biliar dissecado do tecido circundante. Grupo 2 (BDL/Não tratado, n=7) foram submetidos a ligadura do ducto biliar e nenhuma droga foi aplicada. Grupo 3 (BDL/SRL, n=7) receberam dose diária de sirolimo (0,5 mg dia -1 ×kg -1 dissolvido em 1 ml em solução salina) por tubo orogástrico por 14 dias após BDL. Após duas semanas era realizada avaliação bioquímica e histológica. Resultados: Níveis de AST, ALT, AP e TB estavam diminuídos no grupo 3 comparado ao grupo 2. Não houve diferença significante nos níveis séricos de BUN e creatinina em todos os grupos. Observou-se na avaliação histológica evidente fibrose portal e sinais de obstrução do ducto biliar com evidente inflamação portal e lobular. No grupo BDL/SRL verificou-se dano moderado. Lesão tubular foi maior nos subgrupos BDL; entretanto, o grupo 3 mostrou considerável menos lesões nos túbulos e interstício comparados ao grupo 2. No grupo 2 as células epiteliais dos túbulos proximais apresentaram vacúolos e alterações hidrópicas, atrofia e infiltrado celular inflamatório no interstício medula...

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Development of hepatorenal syndrome in bile duct ligated rats

Cited by 36 publications

References 28 publications

Influence of distal ileum exclusion on hepatic and renal functions in presence of extrahepatic cholestasis

Influence of distal ileum exclusion on hepatic and renal functions in presence of extrahepatic cholestasis

Portal hypertension-related inflammatory phenotypes: From a vitelline and amniotic point of view

Effect of sirolimus on renal injury induced by bile duct ligation in rats

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